cra:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
cra |
|---|---|
| Mnemonic |
Fructose |
| Synonyms |
ECK0081, b0080, JW0078, cra, fruC, shl, serR?, fruS?, fruR |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
1.9 minutes |
MG1655: 88028..89032 |
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NC_012967: 90832..91836 |
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NC_012759: 88027..89031 |
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W3110 |
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W3110: 88028..89032 |
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DH10B: 62379..63136 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
fruR(del) (Keio:JW0078) |
deletion |
deletion |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire DL-a-Glycerol |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire DL-Lactate |
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|
fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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|
fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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|
fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire D-Serine |
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fruR(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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fruR11::Tn10 |
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fruR12::kan |
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fruR786(del)::kan |
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CAG45114 fruR786(del)::FRTKanFRT |
deletion |
SigmaE activity |
Decrease in sigmaE activity |
Parental Strain: CAG45114 Experimental Strain: SMR15253 |
See table S11 for full experimental data. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0078 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAACTGGATGAAATCGCTCG Primer 2:CCGCTACGGCTGAGCACGCCGCG | |
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Kohara Phage |
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leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 86% [9] | |
|
Linked marker |
est. P1 cotransduction: 4% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
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EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
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ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 2.0 2.1 2.2 CGSC: The Coli Genetics Stock Center
- ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Ramseier, TM et al. (1995) The global regulatory protein FruR modulates the direction of carbon flow in Escherichia coli. Mol. Microbiol. 16 1157-69 PubMed
- ↑ Crasnier-Mednansky, M et al. (1997) Cra-mediated regulation of Escherichia coli adenylate cyclase. Microbiology (Reading, Engl.) 143 ( Pt 3) 785-92 PubMed
- ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
