clpA:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
clpA |
---|---|
Gene Synonym(s) | |
Product Desc. |
ATP-binding component of serine protease[2][3]; Component of ClpA ATP-dependent protease specificity component and chaperone[3]; ClpAXP[3]; ClpAP[3] ATPase subunit of ClpAP ATP-dependent protease; protease Ti; molecular chaperone[4] |
Product Synonyms(s) |
ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity[1], B0882[2][1], LopD[2][1], CipA[2][1], ClpA[2][1] , ECK0873, JW0866, lopD, b0882 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
clpA |
---|---|
Mnemonic |
Caseinolytic protease |
Synonyms | |
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Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
19.88 minutes |
MG1655: 922487..924763 |
||
NC_012967: 940113..942245 |
||||
NC_012759: 825455..827731 |
||||
W3110 |
|
W3110: 923686..925962 |
||
DH10B: 976415..978691 |
||||
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
922487 |
Edman degradation |
PMID:3049606 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ΔclpA (Keio:JW0866) |
deletion |
deletion |
PMID:16738554 |
||||
clpA319::mini-kan |
PMID:3049606 |
||||||
ΔclpA783::kan |
PMID:16738554 |
| |||||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0866 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCCTCAATCAAGAACTGGAACT Primer 2:CCATGCGCTGCTTCCGCCTTGT | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: % [6] | |
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 41% [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000152 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0154 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003001 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
ClpA |
---|---|
Synonyms |
ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity[1], B0882[2][1], LopD[2][1], CipA[2][1], ClpA[2][1] , ECK0873, JW0866, lopD, b0882 |
Product description |
ATP-binding component of serine protease[2][3]; Component of ClpA ATP-dependent protease specificity component and chaperone[3]; ClpAXP[3]; ClpAP[3] ATPase subunit of ClpAP ATP-dependent protease; protease Ti; molecular chaperone[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005515 |
protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004176 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001270 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003959 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013093 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR018368 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0017111 |
nucleoside-triphosphatase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR003593 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0019538 |
protein metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004176 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of ClpA ATP-dependent protease specificity component and chaperone |
could be indirect |
||
Protein |
mukB |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rho |
PMID:15690043 |
Experiment(s):EBI-884758, EBI-891593 | |
Protein |
rplE |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rplJ |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rpoB |
PMID:15690043 |
Experiment(s):EBI-884758, EBI-879304 | |
Protein |
rpoC |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rpsA |
PMID:15690043 |
Experiment(s):EBI-884758, EBI-891593 | |
Protein |
rpsB |
PMID:15690043 |
Experiment(s):EBI-884758, EBI-891593 | |
Protein |
rpsD |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rpsG |
PMID:15690043 |
Experiment(s):EBI-884758, EBI-891593 | |
Protein |
secA |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
spoT |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
tufA |
PMID:15690043 |
Experiment(s):EBI-884758 | |
Protein |
rpsF |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpsJ |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpsN |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpsP |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpsU |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
sbcC |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
ybjE |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
ygiF |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
yhbY |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
clpS |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
barA |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
dnaK |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
proV |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
purL |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rhsC |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplC |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplI |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplL |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplM |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplR |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rplU |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpmA |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpmC |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
rpmG |
PMID:15690043 |
Experiment(s):EBI-891593 | |
Protein |
ybaA |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
fdnH |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
ppk |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
rplB |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
fhuA |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
yciH |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
cadA |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
cheY |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
nadE |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
rplQ |
PMID:16606699 |
Experiment(s):EBI-1138254 | |
Protein |
rpmG |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
purL |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
ygiF |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsU |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rplU |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsN |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsF |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rplI |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rplR |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rplM |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rhsC |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
sbcC |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpmA |
PMID:19402753 |
LCMS(ID Probability):99.2 | |
Protein |
rpsJ |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rpsA |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | |
Protein |
tufB |
PMID:19402753 |
MALDI(Z-score):22.764725 | |
Protein |
rho |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | |
Protein |
rplD |
PMID:19402753 |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | |
Protein |
yhbY |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
proV |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
clpS |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
ybjE |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
barA |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
rplL |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
Subunits of ClpAXP |
could be indirect |
||
Protein |
Subunits of ClpAP |
could be indirect |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
cytoplasm |
From EcoCyc[3] |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MLNQELELSL NMAFARAREH RHEFMTVEHL LLALLSNPSA REALEACSVD LVALRQELEA FIEQTTPVLP ASEEERDTQP TLSFQRVLQR AVFHVQSSGR NEVTGANVLV AIFSEQESQA AYLLRKHEVS RLDVVNFISH GTRKDEPTQS SDPGSQPNSE EQAGGEERME NFTTNLNQLA RVGGIDPLIG REKELERAIQ VLCRRRKNNP LLVGESGVGK TAIAEGLAWR IVQGDVPEVM ADCTIYSLDI GSLLAGTKYR GDFEKRFKAL LKQLEQDTNS ILFIDEIHTI IGAGAASGGQ VDAANLIKPL LSSGKIRVIG STTYQEFSNI FEKDRALARR FQKIDITEPS IEETVQIING LKPKYEAHHD VRYTAKAVRA AVELAVKYIN DRHLPDKAID VIDEAGARAR LMPVSKRKKT VNVADIESVV ARIARIPEKS VSQSDRDTLK NLGDRLKMLV FGQDKAIEAL TEAIKMARAG LGHEHKPVGS FLFAGPTGVG KTEVTVQLSK ALGIELLRFD MSEYMERHTV SRLIGAPPGY VGFDQGGLLT DAVIKHPHAV LLLDEIEKAH PDVFNILLQV MDNGTLTDNN GRKADFRNVV LVMTTNAGVR ETERKSIGLI HQDNSTDAME EIKKIFTPEF RNRLDNIIWF DHLSTDVIHQ VVDKFIVELQ VQLDQKGVSL EVSQEARNWL AEKGYDRAMG ARPMARVIQD NLKKPLANEL LFGSLVDGGQ VTVALDKEKN ELTYGFQSAQ KHKAEAAH |
Length |
758 |
Mol. Wt |
84.207 kDa |
pI |
6.3 (calculated) |
Extinction coefficient |
32,890 - 33,265 (calc based on 11 Y, 3 W, and 3 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0003001 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000152 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0154 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
2.87E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
197.673+/-0.798 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.11955+/-0.00889 |
Molecules/cell |
|
by FISH |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.322056239 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
989 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
2420 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
928 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
</protect>
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:922467..922507
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
<protect>
Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for clpA | |
microarray |
Summary of data for clpA from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to clpA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0154 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000152 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003001 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
Shigella flexneri |
CLPA |
From SHIGELLACYC |
E. coli O157 |
CLPA |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
PF00004 ATPase family associated with various cellular activities (AAA) |
||
EcoCyc:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10156 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000152 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0154 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003001 |
Escherichia coli str. K-12 substr. MG1655 | |
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<protect></protect>
Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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