clpA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

clpA

Gene Synonym(s)

ECK0873, b0882, JW0866, lopD, cipA[1][2]

Product Desc.

ATP-binding component of serine protease[2][3];

Component of ClpA ATP-dependent protease specificity component and chaperone[3]; ClpAXP[3]; ClpAP[3]

ATPase subunit of ClpAP ATP-dependent protease; protease Ti; molecular chaperone[4]

Product Synonyms(s)

ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity[1], B0882[2][1], LopD[2][1], CipA[2][1], ClpA[2][1] , ECK0873, JW0866, lopD, b0882

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): clpA[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

clpA

Mnemonic

Caseinolytic protease

Synonyms

ECK0873, b0882, JW0866, lopD, cipA[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

19.88 minutes 

MG1655: 922487..924763
<gbrowseImage> name=NC_000913:922487..924763 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 940113..942245
<gbrowseImage> name=NC_012967:940113..942245 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 825455..827731
<gbrowseImage> name=NC_012759:825455..827731 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 923686..925962
<gbrowseImage> name=NC_007779:923686..925962 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 976415..978691
<gbrowseImage> name=NC_010473:976415..978691 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

922487

Edman degradation

PMID:3049606


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔclpA (Keio:JW0866)

deletion

deletion

PMID:16738554

Shigen
CGSC8898[5]

clpA319::mini-kan

PMID:3049606

CGSC:90909

ΔclpA783::kan

PMID:16738554

CGSC:100338


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0866

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCTCAATCAAGAACTGGAACT

Primer 2:CCATGCGCTGCTTCCGCCTTGT

1E5

Kohara Phage

Genobase

PMID:3038334

1H1

Kohara Phage

Genobase

PMID:3038334

1F10

Kohara Phage

Genobase

PMID:3038334

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[5]

est. P1 cotransduction: % [6]
Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[5]

est. P1 cotransduction: 41% [6]
Synonyms:zbj-1230::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10156

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10156

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000152

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945764

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0154

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003001

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ClpA

Synonyms

ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity[1], B0882[2][1], LopD[2][1], CipA[2][1], ClpA[2][1] , ECK0873, JW0866, lopD, b0882

Product description

ATP-binding component of serine protease[2][3];

Component of ClpA ATP-dependent protease specificity component and chaperone[3]; ClpAXP[3]; ClpAP[3]

ATPase subunit of ClpAP ATP-dependent protease; protease Ti; molecular chaperone[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004176

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001270

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003959

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013093

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018368

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0019538

protein metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004176

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of ClpA ATP-dependent protease specificity component and chaperone

could be indirect

Protein

mukB

PMID:15690043

Experiment(s):EBI-884758

Protein

rho

PMID:15690043

Experiment(s):EBI-884758, EBI-891593

Protein

rplE

PMID:15690043

Experiment(s):EBI-884758

Protein

rplJ

PMID:15690043

Experiment(s):EBI-884758

Protein

rpoB

PMID:15690043

Experiment(s):EBI-884758, EBI-879304

Protein

rpoC

PMID:15690043

Experiment(s):EBI-884758

Protein

rpsA

PMID:15690043

Experiment(s):EBI-884758, EBI-891593

Protein

rpsB

PMID:15690043

Experiment(s):EBI-884758, EBI-891593

Protein

rpsD

PMID:15690043

Experiment(s):EBI-884758

Protein

rpsG

PMID:15690043

Experiment(s):EBI-884758, EBI-891593

Protein

secA

PMID:15690043

Experiment(s):EBI-884758

Protein

spoT

PMID:15690043

Experiment(s):EBI-884758

Protein

tufA

PMID:15690043

Experiment(s):EBI-884758

Protein

rpsF

PMID:15690043

Experiment(s):EBI-891593

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-891593

Protein

rpsN

PMID:15690043

Experiment(s):EBI-891593

Protein

rpsP

PMID:15690043

Experiment(s):EBI-891593

Protein

rpsU

PMID:15690043

Experiment(s):EBI-891593

Protein

sbcC

PMID:15690043

Experiment(s):EBI-891593

Protein

ybjE

PMID:15690043

Experiment(s):EBI-891593

Protein

ygiF

PMID:15690043

Experiment(s):EBI-891593

Protein

yhbY

PMID:15690043

Experiment(s):EBI-891593

Protein

clpS

PMID:15690043

Experiment(s):EBI-891593

Protein

barA

PMID:15690043

Experiment(s):EBI-891593

Protein

dnaK

PMID:15690043

Experiment(s):EBI-891593

Protein

proV

PMID:15690043

Experiment(s):EBI-891593

Protein

purL

PMID:15690043

Experiment(s):EBI-891593

Protein

rhsC

PMID:15690043

Experiment(s):EBI-891593

Protein

rplC

PMID:15690043

Experiment(s):EBI-891593

Protein

rplI

PMID:15690043

Experiment(s):EBI-891593

Protein

rplL

PMID:15690043

Experiment(s):EBI-891593

Protein

rplM

PMID:15690043

Experiment(s):EBI-891593

Protein

rplR

PMID:15690043

Experiment(s):EBI-891593

Protein

rplU

PMID:15690043

Experiment(s):EBI-891593

Protein

rpmA

PMID:15690043

Experiment(s):EBI-891593

Protein

rpmC

PMID:15690043

Experiment(s):EBI-891593

Protein

rpmG

PMID:15690043

Experiment(s):EBI-891593

Protein

ybaA

PMID:16606699

Experiment(s):EBI-1138254

Protein

fdnH

PMID:16606699

Experiment(s):EBI-1138254

Protein

ppk

PMID:16606699

Experiment(s):EBI-1138254

Protein

rplB

PMID:16606699

Experiment(s):EBI-1138254

Protein

fhuA

PMID:16606699

Experiment(s):EBI-1138254

Protein

yciH

PMID:16606699

Experiment(s):EBI-1138254

Protein

cadA

PMID:16606699

Experiment(s):EBI-1138254

Protein

cheY

PMID:16606699

Experiment(s):EBI-1138254

Protein

nadE

PMID:16606699

Experiment(s):EBI-1138254

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1138254

Protein

rpmG

PMID:19402753

LCMS(ID Probability):99.6

Protein

purL

PMID:19402753

LCMS(ID Probability):99.6

Protein

ygiF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsU

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplU

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplI

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplR

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753

LCMS(ID Probability):99.6

Protein

rhsC

PMID:19402753

LCMS(ID Probability):99.6

Protein

sbcC

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmA

PMID:19402753

LCMS(ID Probability):99.2

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

tufB

PMID:19402753

MALDI(Z-score):22.764725

Protein

rho

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.6

Protein

proV

PMID:19402753

LCMS(ID Probability):99.6

Protein

clpS

PMID:19402753

LCMS(ID Probability):99.6

Protein

ybjE

PMID:19402753

LCMS(ID Probability):99.6

Protein

barA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of ClpAXP

could be indirect

Protein

Subunits of ClpAP

could be indirect


</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MLNQELELSL NMAFARAREH RHEFMTVEHL LLALLSNPSA REALEACSVD LVALRQELEA
FIEQTTPVLP ASEEERDTQP TLSFQRVLQR AVFHVQSSGR NEVTGANVLV AIFSEQESQA
AYLLRKHEVS RLDVVNFISH GTRKDEPTQS SDPGSQPNSE EQAGGEERME NFTTNLNQLA
RVGGIDPLIG REKELERAIQ VLCRRRKNNP LLVGESGVGK TAIAEGLAWR IVQGDVPEVM
ADCTIYSLDI GSLLAGTKYR GDFEKRFKAL LKQLEQDTNS ILFIDEIHTI IGAGAASGGQ
VDAANLIKPL LSSGKIRVIG STTYQEFSNI FEKDRALARR FQKIDITEPS IEETVQIING
LKPKYEAHHD VRYTAKAVRA AVELAVKYIN DRHLPDKAID VIDEAGARAR LMPVSKRKKT
VNVADIESVV ARIARIPEKS VSQSDRDTLK NLGDRLKMLV FGQDKAIEAL TEAIKMARAG
LGHEHKPVGS FLFAGPTGVG KTEVTVQLSK ALGIELLRFD MSEYMERHTV SRLIGAPPGY
VGFDQGGLLT DAVIKHPHAV LLLDEIEKAH PDVFNILLQV MDNGTLTDNN GRKADFRNVV
LVMTTNAGVR ETERKSIGLI HQDNSTDAME EIKKIFTPEF RNRLDNIIWF DHLSTDVIHQ
VVDKFIVELQ VQLDQKGVSL EVSQEARNWL AEKGYDRAMG ARPMARVIQD NLKKPLANEL
LFGSLVDGGQ VTVALDKEKN ELTYGFQSAQ KHKAEAAH
Length

758

Mol. Wt

84.207 kDa

pI

6.3 (calculated)

Extinction coefficient

32,890 - 33,265 (calc based on 11 Y, 3 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

210..347

PF00004 ATPase family associated with various cellular activities (AAA)

PMID:19920124

Domain

13..63

PF02861 Clp amino terminal domain

PMID:19920124

Domain

486..647

PF07724 AAA domain (Cdc48 subfamily)

PMID:19920124

Domain

653..742

PF10431 C-terminal, D2-small domain, of ClpB protein

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=clpA taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128850

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945764

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003001

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABH9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10156

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10156

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945764

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000152

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0154

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

2.87E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

197.673+/-0.798

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.11955+/-0.00889

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.322056239

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

989

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2420

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

928

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

clpA

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:922467..922507 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0882 (EcoliWiki Page)

NCBI GEO profiles for clpA

microarray

GenExpDB:b0882 (EcoliWiki Page)

Summary of data for clpA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to clpA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10156

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0154

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0882

EcoGene

EcoGene:EG10156

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000152

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003001

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Tetraodon nigroviridis

  • GSTENP00038721001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CLPA

From SHIGELLACYC

E. coli O157

CLPA

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00004 ATPase family associated with various cellular activities (AAA)

Pfam (EcoliWiki Page)

PF02861 Clp amino terminal domain

Panther (EcoliWiki Page)

PTHR11638:SF14

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Superfamily (EcoliWiki Page)

SUPERFAMILY:81923

Pfam (EcoliWiki Page)

PF07724 AAA domain (Cdc48 subfamily)

Pfam (EcoliWiki Page)

PF10431 C-terminal, D2-small domain, of ClpB protein

PFAM (EcoliWiki Page)

PFAM:PF02861

EcoCyc

EcoCyc:EG10156

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10156

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000152

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0154

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003001

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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