cheB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

cheB

Gene Synonym(s)

ECK1884, b1883, JW1872[1], JW1872

Product Desc.

protein-glutamate methylesterase in two-component regulatory system with CheA[1] Chemotaxis MCP protein-glutamate methylesterase; reverses CheR methylation at specific MCP glutamates[2]

Product Synonyms(s)

B1883[3][1], ECK1884, JW1872, b1883

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): tar-tap-cheRBYZ[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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otherPDB (S. typhimurium): 1CHD, 1A2O. N-terminal response regulator domain inhibits C-terminal methylesterase unless phosphorylated at D56 by CheA. Flagellar regulon.[2]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cheB

Mnemonic

Chemotaxis

Synonyms

ECK1884, b1883, JW1872[1], JW1872

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

42.36 minutes 

MG1655: 1966525..1965476
<gbrowseImage> name=NC_000913:1965476..1966525 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1947271..1946222
<gbrowseImage> name=NC_012967:1946222..1947271 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1857535..1858584
<gbrowseImage> name=NC_012759:1857535..1858584 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1970215..1969166
<gbrowseImage> name=NC_007779:1969166..1970215 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2057096..2056047
<gbrowseImage> name=NC_010473:2056047..2057096 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcheB (Keio:JW1872)

deletion

deletion

PMID:16738554

Shigen
CGSC9558[4]

cheB::Tn5KAN-2 (FB20487)

Insertion at nt 407 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20487

contains pKD46

ΔcheB736::kan

PMID:16738554

CGSC:100298


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1872

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCAAAATCAGGGTGTTATC

Primer 2:CCAATACGTATCGCCTGTCCGGC

12C7

Kohara Phage

Genobase

PMID:3038334

7F11

Kohara Phage

Genobase

PMID:3038334

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[4]

est. P1 cotransduction: 24% [5]

uvrC279::Tn10

Linked marker

CAG12156 = CGSC7394[4]

est. P1 cotransduction: 39% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10147

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10147

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000143

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946394

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0145

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006284

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CheB

Synonyms

B1883[3][1], ECK1884, JW1872, b1883

Product description

protein-glutamate methylesterase in two-component regulatory system with CheA[1] Chemotaxis MCP protein-glutamate methylesterase; reverses CheR methylation at specific MCP glutamates[2]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000156

two-component response regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000673

F

Seeded from EcoCyc (v14.0)

complete

GO:0000156

two-component response regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

F

Seeded from EcoCyc (v14.0)

complete

GO:0000156

two-component response regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008248

F

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000673

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

P

Seeded from EcoCyc (v14.0)

complete

GO:0000160

two-component signal transduction system (phosphorelay)

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008248

P

Seeded from EcoCyc (v14.0)

complete

GO:0004871

signal transducer activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000673

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008248

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001789

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000673

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008248

P

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0145

P

Seeded from EcoCyc (v14.0)

complete

GO:0007606

sensory perception of chemical stimulus

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

P

Seeded from EcoCyc (v14.0)

complete

GO:0008984

protein-glutamate methylesterase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00099

F

Seeded from EcoCyc (v14.0)

complete

GO:0008984

protein-glutamate methylesterase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000673

F

Seeded from EcoCyc (v14.0)

complete

GO:0008984

protein-glutamate methylesterase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008248

F

Seeded from EcoCyc (v14.0)

complete

GO:0008984

protein-glutamate methylesterase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.1.61

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0006935

chemotaxis

PMID:4895809

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

fumB

PMID:16606699

Experiment(s):EBI-1141171

Protein

mngB

PMID:16606699

Experiment(s):EBI-1141171

Protein

nadE

PMID:16606699

Experiment(s):EBI-1141171

Protein

mutS

PMID:16606699

Experiment(s):EBI-1141171

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1141171

Protein

uvrY

PMID:16606699

Experiment(s):EBI-1141171

Protein

dnaK

PMID:16606699

Experiment(s):EBI-1141171

Protein

ptsI

PMID:16606699

Experiment(s):EBI-1141171
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[6]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSKIRVLSVD DSALMRQIMT EIINSHSDME MVATAPDPLV ARDLIKKFNP DVLTLDVEMP
RMDGLDFLEK LMRLRPMPVV MVSSLTGKGS EVTLRALELG AIDFVTKPQL GIREGMLAYN
EMIAEKVRTA AKASLAAHKP LSAPTTLKAG PLLSSEKLIA IGASTGGTEA IRHVLQPLPL
SSPALLITQH MPPGFTRSFA DRLNKLCQIG VKEAEDGERV LPGHAYIAPG DRHMELSRSG
ANYQIKIHDG PAVNRHRPSV DVLFHSVAKQ AGRNAVGVIL TGMGNDGAAG MLAMRQAGAW
TLAQNEASCV VFGMPREAIN MGGVCEVVDL SQVSQQMLAK ISAGQAIRI
Length

349

Mol. Wt

37.467 kDa

pI

8.4 (calculated)

Extinction coefficient

9,970 - 10,345 (calc based on 3 Y, 1 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

158..340

PF01339 CheB methylesterase

PMID:19920124

Domain

6..118

PF00072 Response regulator receiver domain

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cheB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129835

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946394

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006284

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P07330

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10147

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10147

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946394

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000143

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0145

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

1a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

1a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

tar-tap-cheRBYZ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1966505..1966545 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1883 (EcoliWiki Page)

NCBI GEO profiles for cheB

microarray

GenExpDB:b1883 (EcoliWiki Page)

Summary of data for cheB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to cheB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10147

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0145

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1883

EcoGene

EcoGene:EG10147

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000143

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006284

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000028734 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

CHEB

From SHIGELLACYC

E. coli O157

CHEB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01339 CheB methylesterase

Superfamily (EcoliWiki Page)

SUPERFAMILY:52172

Superfamily (EcoliWiki Page)

SUPERFAMILY:52738

Pfam (EcoliWiki Page)

PF00072 Response regulator receiver domain

EcoCyc

EcoCyc:EG10147

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10147

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000143

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0145

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006284

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

Categories

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