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Standard Name	betI
Gene Synonym(s)	ECK0311, b0313, JW0305^[1], JW0305
Product Desc.	BetI transcriptional repressor^[2]^[3] Repressor for the betIBA betT divergon, choline-sensing; bet genes confer protection against osmotic stress by making the osmoprotectant glycine betaine from choline^[4]
Product Synonyms(s)	DNA-binding transcriptional repressor^[1], B0313^[2]^[1], BetI^[2]^[1] , ECK0311, JW0305, b0313
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): betIBA^[2], OP00155
Regulation/Activity
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Notes

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TetR family. ArcA regulon. BetI is bound to its operator in both repressed and induced cells. A divergon is a regulon comprised of two adjacent divergent operons or genes. The bet genes are induced by choline, oxygen, and osmotic stress.^[4]

Gene

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Nomenclature

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Standard name	betI
Mnemonic	Betaine
Synonyms	ECK0311, b0313, JW0305^[1], JW0305
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.07 minutes	MG1655: 328558..327971 <gbrowseImage> name=NC_000913:327971..328558 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 301983..301396 <gbrowseImage> name=NC_012967:301396..301983 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 328558..327971 <gbrowseImage> name=NC_007779:327971..328558 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 304108..303521 <gbrowseImage> name=NC_010473:303521..304108 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
betI(del) (Keio:JW0305)	deletion	deletion	PMID:16738554	Shigen CGSC8505^[5]
betI::Tn5KAN-2 (FB20139)	Insertion at nt 246 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20139	does not contain pKD46
betI755(del)::kan			PMID:16738554	CGSC:100178
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0305	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCCAAATTGGGGATGCAGTC Primer 2:CCATCGGTGGGTAGATGCTGAGT
6E6	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: 2% ^[6]
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[5]	est. P1 cotransduction: 20% ^[6] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10111	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10111	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000107	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944981	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0109	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001076	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	BetI
Synonyms	DNA-binding transcriptional repressor^[1], B0313^[2]^[1], BetI^[2]^[1] , ECK0311, JW0305, b0313
Product description	BetI transcriptional repressor^[2]^[3] Repressor for the betIBA betT divergon, choline-sensing; bet genes confer protection against osmotic stress by making the osmoprotectant glycine betaine from choline^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00768	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001647	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001647	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019285	glycine betaine biosynthetic process from choline	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00768	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012287	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045449	regulation of transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0805	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045892	negative regulation of transcription, DNA-dependent	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00768	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	hchA	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	zwf	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	glpD	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	prs	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	nadE	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	betB	PMID:16606699^[7]	Experiment(s):EBI-1136185
Protein	sapA	PMID:16606699^[7]	Experiment(s):EBI-1136185
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPKLGMQSIR RRQLIDATLE AINEVGMHDA TIAQIARRAG VSTGIISHYF RDKNGLLEAT MRDITSQLRD AVLNRLHALP QGSAEQRLQA IVGGNFDETQ VSSAAMKAWL AFWASSMHQP MLYRLQQVSS RRLLSNLVSE FRRELPREQA QEAGYGLAAL IDGLWLRAAL SGKPLDKTRA NSLTRHFITQ HLPTD
Length	195
Mol. Wt	21.815 kDa
pI	10.6 (calculated)
Extinction coefficient	20,970 (calc based on 3 Y, 3 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	14..60	PF00440 Bacterial regulatory proteins, tetR family		PMID:19920124^[8]
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<motif_map/>

Structure
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Structures

<beststructure> gene=betI taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176011	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944981	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001076	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P17446	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10111	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10111	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944981	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000107	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0109	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	59	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	39	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	59^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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Transcription unit(s)
betIBA
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Notes

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Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:328538..328578 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

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Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0313 (EcoliWiki Page)	NCBI GEO profiles for betI
microarray	GenExpDB:b0313 (EcoliWiki Page)	Summary of data for betI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to betI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10111	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0109	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0313
EcoGene	EcoGene:EG10111	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000107	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001076	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Notes

Families

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
E. coli O157	BETI	From ECOO157CYC
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Notes

Families

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See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00440 Bacterial regulatory proteins, tetR family
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 ^7.5 ^7.6 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

betI:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to betI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Families

Notes

Links

References

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