asnC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

asnC

Gene Synonym(s)

ECK3737, b3743, JW3721[1], JW3721

Product Desc.

AsnC transcriptional repressor[2]; AsnC transcriptional dual regulator[3]

Regulatory gene for asnA, asnC, gidA, Lrp family[4]

Product Synonyms(s)

DNA-binding transcriptional dual regulator[1], B3743[2][1], AsnC[2][1] , ECK3737, JW3721, b3743

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): asnC[2], OP00153, asnC-mioC-mnmG[3], asnC-mioC-gidA

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Feast/famine regulatory protein.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

asnC

Mnemonic

Asparagine

Synonyms

ECK3737, b3743, JW3721[1], JW3721

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

84.59 minutes 

MG1655: 3925026..3924568
<gbrowseImage> name=NC_000913:3924568..3925026 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3887364..3886906
<gbrowseImage> name=NC_012967:3886906..3887364 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3812901..3813359
<gbrowseImage> name=NC_012759:3812901..3813359 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3709678..3710136
<gbrowseImage> name=NC_007779:3709678..3710136 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4022610..4022152
<gbrowseImage> name=NC_010473:4022152..4022610 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔasnC (Keio:JW3721)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen CGSC10718[5]

asnC::Tn5KAN-I-SceI (FB21431)

Insertion at nt 207 in Minus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB21431

contains pKD46

asnC::Tn5KAN-I-SceI (FB21432)

Insertion at nt 207 in Minus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB21432

does not contain pKD46

ΔasnC772::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:100108


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3721

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGAAAATTATCTGATCGACAA

Primer 2:CCGGGCTTGATGGTACGCATGAT

2A1

Kohara Phage

Genobase

PMID:3038334

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 10% [6]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[5]

est. P1 cotransduction: 84% [6]
Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10093

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10093

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000089

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948259

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0091

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012237

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AsnC

Synonyms

DNA-binding transcriptional dual regulator[1], B3743[2][1], AsnC[2][1] , ECK3737, JW3721, b3743

Product description

AsnC transcriptional repressor[2]; AsnC transcriptional dual regulator[3]

Regulatory gene for asnA, asnC, gidA, Lrp family[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000485

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019885

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019887

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019888

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

PMID:2864330

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000485

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019885

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019887

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019888

C

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:2864330

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

F

Seeded from EcoCyc (v14.0)

complete

GO:0016566

specific transcriptional repressor activity

PMID:2864330

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016566

specific transcriptional repressor activity

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

F

Seeded from EcoCyc (v14.0)

complete

GO:0016597

amino acid binding

PMID:16528101

IPI: Inferred from Physical Interaction

KEGG_LIGAND:C00152

F

Seeded from EcoCyc (v14.0)

complete

GO:0032582

negative regulation of gene-specific transcription

PMID:2864330

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0032582

negative regulation of gene-specific transcription

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

P

Seeded from EcoCyc (v14.0)

complete

GO:0043193

positive regulation of gene-specific transcription

PMID:2864330

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0043193

positive regulation of gene-specific transcription

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

P

Seeded from EcoCyc (v14.0)

complete

GO:0043200

response to amino acid stimulus

PMID:2864330

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000485

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019885

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019887

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019888

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

PMID:2864330

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P03040
UniProtKB:P03034

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

hchA

PMID:16606699

Experiment(s):EBI-1146629

Protein

asnA

asnC is a transcriptional activator of asnA

PMID:2836709


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MENYLIDNLD RGILEALMGN ARTAYAELAK QFGVSPGTIH VRVEKMKQAG IITGARIDVS
PKQLGYDVGC FIGIILKSAK DYPSALAKLE SLDEVTEAYY TTGHYSIFIK VMCRSIDALQ
HVLINKIQTI DEIQSTETLI VLQNPIMRTI KP
Length

152

Mol. Wt

16.888 kDa

pI

6.7 (calculated)

Extinction coefficient

10,430 - 10,680 (calc based on 7 Y, 0 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

72..144

PF01037 AsnC family

PMID:19920124

Domain

11..67

PF08279 HTH domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=asnC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131611

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948259

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012237

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACI6

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10093

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10093

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948259

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000089

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0091

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

78.409+/-0.434

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.006550218

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

694

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

156

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

300

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

asnC

asnC-mioC-mnmG

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3925006..3925046 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3743 (EcoliWiki Page)

NCBI GEO profiles for asnC

microarray

GenExpDB:b3743 (EcoliWiki Page)

Summary of data for asnC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3924533..3924851) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ01; Well:D5[7]

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Notes

Accessions Related to asnC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10093

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0091

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3743

EcoGene

EcoGene:EG10093

Escherichia coli str. K-12 substr. MG1655

EcoCyc (EcoliWiki Page)

EcoCyc:TU0-14262

RegulonDB

RegulonDB:ECK120000089

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012237

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000000095 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ASNC

From SHIGELLACYC

E. coli O157

ASNC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01037 AsnC family

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:54909

Pfam (EcoliWiki Page)

PF08279 HTH domain

EcoCyc

EcoCyc:EG10093

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10093

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000089

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0091

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012237

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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