argP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

argP

Gene Synonym(s)

ECK2912, b2916, JW2883, iciA[1][2], can, canR, iciA

Product Desc.

ArgP transcriptional activator[3][4]

Arginine and lysine responsive transcriptional regulator; inhibitor of chromosome initiation; positive regulator of argK, negative autoregulator in presence of arginine; oriC iteron (13-mer) binding protein, competes with DnaA for iteron binding; activate[5]

Product Synonyms(s)

DNA-binding transcriptional activator, replication initiation inhibitor[1], B2916[3][1], ArgP[3][1], IciA[3][1][2] , can, canR, ECK2912, iciA, JW2883, b2916

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): argP[3], iciA

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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ArgP(IciA) is a LysR family transcriptional regulator. argP mutants are canavanine sensitive. argP is in the pho regulon. ArgP activates the dapB gene in te absense of lysine (Bouvier, 2008).[5]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argP

Mnemonic

Arginine

Synonyms

ECK2912, b2916, JW2883, iciA[1][2], can, canR, iciA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

65.9 minutes 

MG1655: 3057775..3058668
<gbrowseImage> name=NC_000913:3057775..3058668 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2944923..2945816
<gbrowseImage> name=NC_012759:2944923..2945816 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3058409..3059302
<gbrowseImage> name=NC_007779:3058409..3059302 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3151645..3152538
<gbrowseImage> name=NC_010473:3151645..3152538 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3057775

Edman degradation

PMID:2034653


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔargP (Keio:JW2883)

deletion

deletion

Auxotrophies

PMID:16738554

Shigen

CGSC10236[6]

iciA::Tn5KAN-I-SceI (FB20973)

Insertion at nt 408 in Plus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB20973

contains pKD46

iciA::Tn5KAN-I-SceI (FB20974)

Insertion at nt 408 in Plus orientation

Auxotrophies

PMID:15262929

E. coli Genome Project:FB20974

does not contain pKD46

argP55

Auxotrophies

CGSC:5170

ΔargP768::kan

deletion

deletion

Auxotrophies

PMID:16738554

CGSC:100052


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2883

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAACGCCCGGACTACAGAAC

Primer 2:CCATCCTGACGAAGGACTTTGTG

1A2

Kohara Phage

Genobase

PMID:3038334

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[6]

est. P1 cotransduction: % [7]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[6]

est. P1 cotransduction: 35% [7]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10490

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10490

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000483

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944867

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0485

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArgP

Synonyms

DNA-binding transcriptional activator, replication initiation inhibitor[1], B2916[3][1], ArgP[3][1], IciA[3][1][2] , can, canR, ECK2912, iciA, JW2883, b2916

Product description

ArgP transcriptional activator[3][4]

Arginine and lysine responsive transcriptional regulator; inhibitor of chromosome initiation; positive regulator of argK, negative autoregulator in presence of arginine; oriC iteron (13-mer) binding protein, competes with DnaA for iteron binding; activate[5]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000847

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

PMID:10600368

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

PMID:2034653

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P12529
UniProtKB:P0A2Q4
UniProtKB:P10183

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

PMID:10600368

IDA: Inferred from Direct Assay

F

complete

GO:0016563

transcription activator activity

PMID:10600368

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016563

transcription activator activity

PMID:2034653

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P12529
UniProtKB:P0A2Q4
UniProtKB:P10183

F

Seeded from EcoCyc (v14.0)

complete

GO:0016566

specific transcriptional repressor activity

PMID:10600368

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016566

specific transcriptional repressor activity

PMID:2034653

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P12529
UniProtKB:P0A2Q4
UniProtKB:P10183

F

Seeded from EcoCyc (v14.0)

complete

GO:0032297

negative regulation of DNA-dependent DNA replication initiation

PMID:1429656

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0032582

negative regulation of gene-specific transcription

PMID:10600368

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0032582

negative regulation of gene-specific transcription

PMID:2034653

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P12529
UniProtKB:P0A2Q4
UniProtKB:P10183

P

Seeded from EcoCyc (v14.0)

complete

GO:0043193

positive regulation of gene-specific transcription

PMID:10600368

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0043193

positive regulation of gene-specific transcription

PMID:2034653

ISS: Inferred from Sequence or Structural Similarity

UniProtKB:P12529
UniProtKB:P0A2Q4
UniProtKB:P10183

P

Seeded from EcoCyc (v14.0)

complete

GO:0003681

bent DNA binding

PMID:10551881

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

nanR

PMID:16606699

Experiment(s):EBI-1144289

Protein

glgB

PMID:16606699

Experiment(s):EBI-1144289

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1144289

Protein

groL

PMID:15690043

Experiment(s):EBI-888615

Protein

tufA

PMID:15690043

Experiment(s):EBI-888615

Protein

groL

PMID:19402753

MALDI(Z-score):17.053160

Protein

groS

PMID:19402753

MALDI(Z-score):23.054402

Protein

tufB

PMID:19402753

MALDI(Z-score):25.460787

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKRPDYRTLQ ALDAVIRERG FERAAQKLCI TQSAVSQRIK QLENMFGQPL LVRTVPPRPT
EQGQKLLALL RQVELLEEEW LGDEQTGSTP LLLSLAVNAD SLATWLLPAL APVLADSPIR
LNLQVEDETR TQERLRRGEV VGAVSIQHQA LPSCLVDKLG ALDYLFVSSK PFAEKYFPNG
VTRSALLKAP VVAFDHLDDM HQAFLQQNFD LPPGSVPCHI VNSSEAFVQL ARQGTTCCMI
PHLQIEKELA SGELIDLTPG LFQRRMLYWH RFAPESRMMR KVTDALLDYG HKVLRQD
Length

297

Mol. Wt

33.471 kDa

pI

6.9 (calculated)

Extinction coefficient

23,950 - 24,575 (calc based on 5 Y, 3 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

88..290

PF03466 LysR substrate binding domain

PMID:19920124

Domain

6..64

PF00126 Bacterial regulatory helix-turn-helix protein, lysR family

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=argP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purification protocol

PMID: 10600368

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130817

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944867

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009574

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8S1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10490

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10490

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944867

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000483

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0485

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

33.368+/-0.652

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

Protein

E. coli K-12 MG1655

789

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

624

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

672

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

argP

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3057755..3057795 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2916 (EcoliWiki Page)

NCBI GEO profiles for argP

microarray

GenExpDB:b2916 (EcoliWiki Page)

Summary of data for argP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3057534..3057807) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:G4[8]

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Notes

Accessions Related to argP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10490

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0485

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2916

EcoGene

EcoGene:EG10490

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000483

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009574

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

ICIA

From SHIGELLACYC

E. coli O157

ICIA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03466 LysR substrate binding domain

Pfam (EcoliWiki Page)

PF00126 Bacterial regulatory helix-turn-helix protein, lysR family

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:53850

EcoCyc

EcoCyc:EG10490

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10490

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000483

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0485

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 Celis, RT (1999) Repression and activation of arginine transport genes in Escherichia coli K 12 by the ArgP protein. J. Mol. Biol. 294 1087-95 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  5. ↑ 5.0 5.1 5.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  6. ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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