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Expression|

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Standard Name	aldB
Gene Synonym(s)	ECK3577, b3588, JW3561, yiaX^[1], yiaX
Product Desc.	ALDDEHYDROGB^[2]^[3]; Component of acetaldehyde dehydrogenase^[3] Aldehyde dehydrogenase, NADP-dependent; tetrameric^[4]
Product Synonyms(s)	aldehyde dehydrogenase B^[1], B3588^[2]^[1], YiaX^[2]^[1], AldB^[2]^[1] , ECK3577, JW3561, yiaX, b3588
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): aldB^[2], OP00186, aldb
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	aldB
Mnemonic	Aldehyde dehydrogenase
Synonyms	ECK3577, b3588, JW3561, yiaX^[1], yiaX
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	80.89 minutes	MG1655: 3754534..3752996 <gbrowseImage> name=NC_000913:3752996..3754534 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3691204..3689666 <gbrowseImage> name=NC_012967:3689666..3691204 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3641322..3642860 <gbrowseImage> name=NC_012759:3641322..3642860 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3883904..3885442 <gbrowseImage> name=NC_007779:3883904..3885442 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3852111..3850573 <gbrowseImage> name=NC_010473:3850573..3852111 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3752996	Edman degradation	PMID:15659684
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
aldBR197E		R197E	Less than 10% of wild-type acetaldehyde dehydrogenase activity			seeded from UniProt:P37685
ΔaldB (Keio:JW3561)	deletion	deletion		PMID:16738554	Shigen CGSC10626^[5]
aldB::Tn5KAN-I-SceI (FB21320)	Insertion at nt 603 in Plus orientation			PMID:15262929	E. coli Genome Project:FB21320	does not contain pKD46
aldB::Tn5KAN-I-SceI (FB21321)	Insertion at nt 603 in Plus orientation			PMID:15262929	E. coli Genome Project:FB21321	contains pKD46
ΔaldB772::kan				PMID:16738554	CGSC:99964
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
2C10	Kohara Phage	Genobase	PMID:3038334
9B3	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 1% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12292	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002173	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948104	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2200	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011718	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AldB
Synonyms	aldehyde dehydrogenase B^[1], B3588^[2]^[1], YiaX^[2]^[1], AldB^[2]^[1] , ECK3577, JW3561, yiaX, b3588
Product description	ALDDEHYDROGB^[2]^[3]; Component of acetaldehyde dehydrogenase^[3] Aldehyde dehydrogenase, NADP-dependent; tetrameric^[4]
EC number (for enzymes)	1.2.1.22^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0009438	methylglyoxal metabolic process				P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015590	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016160	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016161	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016162	F	Seeded from EcoCyc (v14.0)	complete
	GO:0045471	response to ethanol	PMID:15659684	IDA: Inferred from Direct Assay		P		complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004029	aldehyde dehydrogenase (NAD) activity	PMID:15659684	IDA: Inferred from Direct Assay		F		complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015590	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	PMID:15659684	IDA: Inferred from Direct Assay		F		complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016160	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016161	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016162	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of acetaldehyde dehydrogenase	could be indirect
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1146177
Protein	ycfD		PMID:19402753	LCMS(ID Probability):99.6
Protein	glcB		PMID:19402753	LCMS(ID Probability):99.6
Protein	aspC		PMID:19402753	LCMS(ID Probability):99.3
Protein	gatA		PMID:19402753	LCMS(ID Probability):99.6
Protein	fur		PMID:19402753	LCMS(ID Probability):99.6
Protein	acnB		PMID:19402753	LCMS(ID Probability):99.6
Protein	gabD		PMID:19402753	LCMS(ID Probability):99.6
Protein	gcvP		PMID:19402753	LCMS(ID Probability):99.6
Protein	deoA		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIAVVKLLPT GEDFASHVYP CYTSHRKETI MTNNPPSAQI KPGEYGFPLK LKARYDNFIG GEWVAPADGE YYQNLTPVTG QLLCEVASSG KRDIDLALDA AHKVKDKWAH TSVQDRAAIL FKIADRMEQN LELLATAETW DNGKPIRETS AADVPLAIDH FRYFASCIRA QEGGISEVDS ETVAYHFHEP LGVVGQIIPW NFPLLMASWK MAPALAAGNC VVLKPARLTP LSVLLLMEIV GDLLPPGVVN VVNGAGGVIG EYLATSKRIA KVAFTGSTEV GQQIMQYATQ NIIPVTLELG GKSPNIFFAD VMDEEDAFFD KALEGFALFA FNQGEVCTCP SRALVQESIY ERFMERAIRR VESIRSGNPL DSVTQMGAQV SHGQLETILN YIDIGKKEGA DVLTGGRRKL LEGELKDGYY LEPTILFGQN NMRVFQEEIF GPVLAVTTFK TMEEALELAN DTQYGLGAGV WSRNGNLAYK MGRGIQAGRV WTNCYHAYPA HAAFGGYKQS GIGRETHKMM LEHYQQTKCL LVSYSDKPLG LF
Length	542
Mol. Wt	59.695 kDa
pI	5.7 (calculated)
Extinction coefficient	69,790 - 70,790 (calc based on 21 Y, 7 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	33..500	PF00171 Aldehyde dehydrogenase family		PMID:19920124
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<motif_map/>

Structure
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Structures

<beststructure> gene=aldB taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Purification and kinetic properties of AldB described in ^[7]

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111619	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948104	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011718	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37685	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12292	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12292	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948104	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002173	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2200	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	11.944+/-0.127	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.029908973	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	76	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
aldB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3754514..3754554 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3588 (EcoliWiki Page)	NCBI GEO profiles for aldB
microarray	GenExpDB:b3588 (EcoliWiki Page)	Summary of data for aldB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3754148..3754379) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ12; Well:B12^[8]
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Notes

Accessions Related to aldB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12292	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2200	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3588
EcoGene	EcoGene:EG12292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002173	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011718	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Schizosaccharomyces pombe	SPAC9E99c (score: 1.000; bootstrap: 95%)	From Inparanoid:20070104
Shigella flexneri	ALDB	From SHIGELLACYC
E. coli O157	ALDB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00171 Aldehyde dehydrogenase family
Superfamily (EcoliWiki Page)	SUPERFAMILY:53720
EcoCyc	EcoCyc:EG12292	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12292	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002173	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2200	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011718	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Ho, KK & Weiner, H (2005) Isolation and characterization of an aldehyde dehydrogenase encoded by the aldB gene of Escherichia coli. J. Bacteriol. 187 1067-73 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

aldB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to aldB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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