{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	acpT
Gene Synonym(s)	ECK3459, b3475, JW3440, yhhU^[1], yhhU
Product Desc.	holo-[acyl carrier protein] synthase 2^[2]^[3] CoA-phosphopantetheinyltransferase; native apo-protein substrate unknown, possibly a back-up gene for acpS; multicopy suppressor of acpS1, can modify ACP when overexpressed^[4]
Product Synonyms(s)	holo-(acyl carrier protein) synthase 2^[1], B3475^[2]^[1], YhhU^[2]^[1], AcpT^[2]^[1] , ECK3459, JW3440, yhhU, b3475
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): acpT^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

A nonsense mutation making a truncated YejM causes increased outer membrane permeability,TS growth, and reduced of lipid A synthesis; these phenotypes, and the lethality of a null mutation, are suppressible by a multicopy acpT clone (Hirvas, 1997; De Lay, 2008).^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	acpT
Mnemonic	Acyl carrier protein
Synonyms	ECK3459, b3475, JW3440, yhhU^[1], yhhU
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	77.83 minutes	MG1655: 3610992..3611579 <gbrowseImage> name=NC_000913:3610992..3611579 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3543657..3544244 <gbrowseImage> name=NC_012967:3543657..3544244 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3499486..3500073 <gbrowseImage> name=NC_012759:3499486..3500073 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4027446..4026859 <gbrowseImage> name=NC_007779:4026859..4027446 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3708737..3709324 <gbrowseImage> name=NC_010473:3708737..3709324 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔacpT (Keio:JW3440)	deletion	deletion			PMID:16738554	Shigen
acpT over-expression			Auxotrophies	acpT can functionally replace acpS only if over-expressed. Fig. 8, plate 2. Fig. 9, lane 3.	PMID:10625633
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3440	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTATCGGATAGTTCTGGGGAA Primer 2:CCGTTAACTGAATCGATCCATTG
5F2	Kohara Phage	Genobase	PMID:3038334
7H7	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: 49% ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: % ^[6]
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12221	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12221	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002107	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947979	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2135	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011351	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AcpT
Synonyms	holo-(acyl carrier protein) synthase 2^[1], B3475^[2]^[1], YhhU^[2]^[1], AcpT^[2]^[1] , ECK3459, JW3440, yhhU, b3475
Product description	holo-[acyl carrier protein] synthase 2^[2]^[3] CoA-phosphopantetheinyltransferase; native apo-protein substrate unknown, possibly a back-up gene for acpS; multicopy suppressor of acpS1, can modify ACP when overexpressed^[4]
EC number (for enzymes)	2.7.8.7^[1]
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009059	macromolecule biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008278	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	PMID:16824108	IDA: Inferred from Direct Assay		F		complete
	GO:0005625	soluble fraction	PMID:16824108	IDA: Inferred from Direct Assay		C		complete
	GO:0018070	peptidyl-serine phosphopantetheinylation	PMID:8939709	NAS: Non-traceable Author Statement		P		complete
	GO:0008897	holo-(acyl-carrier-protein) synthase activity	PMID:16824108	IMP: Inferred from Mutant Phenotype		F	Substrate is Z4853 from e. coli 0157:H7, Figure 3.	complete
	GO:0006633	fatty acid biosynthetic process	PMID:10625633	IGI: Inferred from Genetic Interaction	EcoliWiki:acpS	P	Fig. 1	complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYRIVLGKVS TLSAAPLPPG LREQAPQGPR RERWLAGRAL LSHTLSPLPE IIYGEQGKPA FAPEMPLWFN LSHSGDDIAL LLSDEGEVGC DIEVIRPRAN WRWLANAVFS LGEHAEMDAV HPDQQLEMFW RIWTRKEAIV KQRGGSAWQI VSVDSTYHSS LSVSHCQLEN LSLAICTPTP FTLTADSVQW IDSVN
Length	195
Mol. Wt	21.767 kDa
pI	5.7 (calculated)
Extinction coefficient	48,470 - 48,845 (calc based on 3 Y, 8 W, and 3 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	88..151	PF01648 4'-phosphopantetheinyl transferase superfamily		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=acpT taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131347	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947979	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011351	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37623	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12221	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12221	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947979	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002107	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2135	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	474	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	125	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	199	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
acpT
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:3610972..3611012 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3475 (EcoliWiki Page)	NCBI GEO profiles for acpT
microarray	GenExpDB:b3475 (EcoliWiki Page)	Summary of data for acpT from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
edit table

Notes

Accessions Related to acpT Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12221	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2135	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3475
EcoGene	EcoGene:EG12221	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002107	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011351	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YHHU	From SHIGELLACYC
E. coli O157	Z4867	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01648 4'-phosphopantetheinyl transferase superfamily
Panther (EcoliWiki Page)	PTHR12215:SF2
Superfamily (EcoliWiki Page)	SUPERFAMILY:56214
Superfamily (EcoliWiki Page)	SUPERFAMILY:56214
EcoCyc	EcoCyc:EG12221	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12221	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002107	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2135	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011351	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

acpT:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to acpT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search