acpT:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

acpT

Mnemonic

Acyl carrier protein

Synonyms

ECK3459, b3475, JW3440, yhhU[1], yhhU

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

77.83 minutes 

MG1655: 3610992..3611579
<gbrowseImage> name=NC_000913:3610992..3611579 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3543657..3544244
<gbrowseImage> name=NC_012967:3543657..3544244 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3499486..3500073
<gbrowseImage> name=NC_012759:3499486..3500073 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4027446..4026859
<gbrowseImage> name=NC_007779:4026859..4027446 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3708737..3709324
<gbrowseImage> name=NC_010473:3708737..3709324 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔacpT (Keio:JW3440)

deletion

deletion

PMID:16738554[2]

Shigen

acpT over-expression

Auxotrophies

acpT can functionally replace acpS only if over-expressed. Fig. 8, plate 2. Fig. 9, lane 3.

PMID:10625633[3]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3440

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTATCGGATAGTTCTGGGGAA

Primer 2:CCGTTAACTGAATCGATCCATTG

5F2

Kohara Phage

Genobase

PMID:3038334[5]

7H7

Kohara Phage

Genobase

PMID:3038334[5]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[6]

est. P1 cotransduction: 49% [7]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[6]

est. P1 cotransduction: % [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12221

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12221

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002107

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947979

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2135

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011351

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Flugel, RS et al. (2000) Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli. J. Biol. Chem. 275 959-68 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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