acnA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

acnA

Gene Synonym(s)

ECK1271, b1276, JW1268, acn[1], acn

Product Desc.

Aconitase A, stationary phase induced; iron-sulfur cluster; apo-enzyme binds mRNA for negative translational autoregulation; negatively regulated by ryhB RNA as part of indirect positive regulation by Fur[2]

Product Synonyms(s)

aconitate hydratase 1[1], B1276[3][1], Acn[3][1], AcnA[3][1] , acn, ECK1271, JW1268, b1276

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): acnA[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

acnA

Mnemonic

Aconitase

Synonyms

ECK1271, b1276, JW1268, acn[1], acn

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

BW2952

NC_012759: 1224704..1227379
<gbrowseImage> name=NC_012759:1224704..1227379 source=BW2952 preset=GeneLocation </gbrowseImage>

MG1655

28.75 minutes 

MG1655: 1333855..1336530
<gbrowseImage> name=NC_000913:1333855..1336530 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1334203..1336878
<gbrowseImage> name=NC_012967:1334203..1336878 source=REL606 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1337545..1340220
<gbrowseImage> name=NC_007779:1337545..1340220 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1423251..1425926
<gbrowseImage> name=NC_010473:1423251..1425926 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1333858

Edman degradation

PMID:1838390


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

S522G

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔacnA (Keio:JW1268)

deletion

deletion

PMID:16738554

Shigen
CGSC9141[4]

ΔacnA::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938

ΔacnA::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

ΔacnA::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

ΔacnA785::kan

PMID:16738554

CGSC:99894


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1268

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCGTCAACCCTACGAGAAGC

Primer 2:CCtTTCAACATATTACGAATGAC

13F9

Kohara Phage

Genobase

PMID:3038334

18B6

Kohara Phage

Genobase

PMID:3038334

trpC83::Tn10

Linked marker

CAG18455 = CGSC7371[4]

est. P1 cotransduction: 56% [5]
Synonyms:trpB83::Tn10

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[4]

est. P1 cotransduction: 24% [5]
Synonyms:zci-233::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11325

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11325

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001296

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946724

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1301

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004283

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AcnA

Synonyms

aconitate hydratase 1[1], B1276[3][1], Acn[3][1], AcnA[3][1] , acn, ECK1271, JW1268, b1276

Product description

Aconitase A, stationary phase induced; iron-sulfur cluster; apo-enzyme binds mRNA for negative translational autoregulation; negatively regulated by ryhB RNA as part of indirect positive regulation by Fur[2]

EC number (for enzymes)

4.2.1.3[1]

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003994

aconitate hydratase activity

PMID:1838390

IDA: Inferred from Direct Assay

F

complete

GO:0003994

aconitate hydratase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.1.3

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006099

tricarboxylic acid cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0816

P

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006249

F

Seeded from EcoCyc (v14.0)

complete

GO:0051539

4 iron, 4 sulfur cluster binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0004

F

Seeded from EcoCyc (v14.0)

complete

GO:0003729

mRNA binding

PMID:10589714

IDA: Inferred from Direct Assay

F

complete

GO:0006099

tricarboxylic acid cycle

PMID:10589714

NAS: Non-traceable Author Statement

P

complete

GO:0006097

glyoxylate cycle

PMID:10589714

NAS: Non-traceable Author Statement

P

complete

GO:0005506

iron ion binding

PMID:12486059

IMP: Inferred from Mutant Phenotype

F

complete

GO:0046872

metal ion binding

PMID:12486059

IDA: Inferred from Direct Assay

F

complete

GO:0051539

4 iron, 4 sulfur cluster binding

PMID:12486059

IDA: Inferred from Direct Assay

F

complete

GO:0006979

response to oxidative stress

PMID:12486059

IDA: Inferred from Direct Assay

P

complete

GO:0009061

anaerobic respiration

PMID:12486059

IDA: Inferred from Direct Assay

P

complete

GO:0005737

cytoplasm

PMID:12486059

IDA: Inferred from Direct Assay

C

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

nadE

PMID:16606699

Experiment(s):EBI-1139437

Protein

gadB

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753

LCMS(ID Probability):99.6

Protein

glgP

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753

LCMS(ID Probability):99.6

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rho

PMID:19402753

LCMS(ID Probability):99.6

Protein

tig

PMID:19402753

LCMS(ID Probability):99.6

Protein

csrA

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE
EDIHALAGWL KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL
SPVDLVIDHS VTVDRFGDDE AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC
HQVNLEYLGK AVWSELQDGE WIAYPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ
PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV VGKFVEFYGD GLDSLPLADR
ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW RNPGDEPIFT
STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY
QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL
AKAKLTPYLD ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI
HPLVKTNWLA SPPLVVAYAL AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ
VSTEMFRKEY AEVFEGTAEW KGINVTRSDT YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH
GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER KDFNSYGSRR GNHEVMMRGT
FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK EYGSGSSRDW
AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN
LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K
Length

891

Mol. Wt

97.678 kDa

pI

5.8 (calculated)

Extinction coefficient

114,710 - 115,585 (calc based on 29 Y, 13 W, and 7 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P25516

Domain

57..562

PF00330 Aconitase family (aconitate hydratase)

PMID:19920124

Domain

689..819

PF00694 Aconitase C-terminal domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=acnA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129237

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946724

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004283

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P25516

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11325

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11325

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946724

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001296

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1301

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

238

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

862

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

186

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

acnA

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1333835..1333875 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1276 (EcoliWiki Page)

NCBI GEO profiles for acnA

microarray

GenExpDB:b1276 (EcoliWiki Page)

Summary of data for acnA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to acnA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11325

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1301

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1276

EcoGene

EcoGene:EG11325

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001296

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004283

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000015921 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000019219 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT2G05710 (score: 1.000; bootstrap: 100%)
  • AT4G35830 (score: 0.614)
  • AT4G26970 (score: 0.604)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000000731 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000003252 (score: 0.243)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00037008 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00000040 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000002619 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000002577 (score: 0.270)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000019418 (score: 1.000; bootstrap: -1%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-031118-76 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0229908 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0024957 (score: 1.000; bootstrap: 100%)
  • FBgn0024958 (score: 0.779)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19525-PA (score: 1.000; bootstrap: 100%)
  • GA18513-PA (score: 0.804)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000003381 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000005019 (score: 0.296)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000353159 (score: 1.000; bootstrap: 100%)
  • ENSP00000258886 (score: 0.152)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000010735 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000005500 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000014741 (score: 0.296)

From Inparanoid:20070104

Mus musculus

  • MGI:87879 (score: 1.000; bootstrap: 100%)
  • MGI:1928268 (score: 0.302)

From Inparanoid:20070104

Oryza gramene

  • Q6YZX6 (score: 1.000; bootstrap: 100%)
  • Q5Z4C3 (score: 0.188)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000035646 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000012550 (score: 0.152)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000008337 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000017900 (score: 0.298)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YLR304C (score: 1.000; bootstrap: 81%)
  • YJL200C (score: 0.458)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC24C96c (score: 1.000; bootstrap: 84%)
  • SPBP4H105 (score: 0.472)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000173787 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000164004 (score: 0.259)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00009462001 (score: 1.000; bootstrap: 100%)
  • GSTENP00016071001 (score: 0.098)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000020189 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ACNA

From SHIGELLACYC

E. coli O157

ACNA

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00694 Aconitase C-terminal domain

Pfam (EcoliWiki Page)

PF00330 Aconitase family (aconitate hydratase)

Superfamily (EcoliWiki Page)

SUPERFAMILY:52016

Panther (EcoliWiki Page)

PTHR11670:SF1

Superfamily (EcoliWiki Page)

SUPERFAMILY:53732

EcoCyc

EcoCyc:EG11325

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11325

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001296

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1301

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004283

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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