acnA:Gene Product(s)
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
AcnA |
---|---|
Synonyms |
aconitate hydratase 1[1], B1276[2][1], Acn[2][1], AcnA[2][1] , acn, ECK1271, JW1268, b1276 |
Product description |
Aconitase A, stationary phase induced; iron-sulfur cluster; apo-enzyme binds mRNA for negative translational autoregulation; negatively regulated by ryhB RNA as part of indirect positive regulation by Fur[3] |
EC number (for enzymes) | |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
GO:0003994 |
aconitate hydratase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
GO:0003994 |
aconitate hydratase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005506 |
iron ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006099 |
tricarboxylic acid cycle |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0051539 |
4 iron, 4 sulfur cluster binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0051539 |
4 iron, 4 sulfur cluster binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003729 |
mRNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
GO:0006099 |
tricarboxylic acid cycle |
NAS: Non-traceable Author Statement |
P |
complete | ||||
GO:0006097 |
glyoxylate cycle |
NAS: Non-traceable Author Statement |
P |
complete | ||||
GO:0005506 |
iron ion binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
GO:0046872 |
metal ion binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
GO:0051539 |
4 iron, 4 sulfur cluster binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
GO:0006979 |
response to oxidative stress |
IDA: Inferred from Direct Assay |
P |
complete | ||||
GO:0009061 |
anaerobic respiration |
IDA: Inferred from Direct Assay |
P |
complete | ||||
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
complete | ||||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
nadE |
Experiment(s):EBI-1139437 | ||
Protein |
gadB |
LCMS(ID Probability):99.6 | ||
Protein |
rplS |
LCMS(ID Probability):99.6 | ||
Protein |
rplX |
LCMS(ID Probability):99.6 | ||
Protein |
glgP |
LCMS(ID Probability):99.6 | ||
Protein |
rpsA |
LCMS(ID Probability):99.6 | ||
Protein |
yhbY |
LCMS(ID Probability):99.6 | ||
Protein |
rpmF |
LCMS(ID Probability):99.6 | ||
Protein |
rho |
LCMS(ID Probability):99.6 | ||
Protein |
tig |
LCMS(ID Probability):99.6 | ||
Protein |
csrA |
LCMS(ID Probability):99.6 | ||
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
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Sequence |
MSSTLREASK DTLQAKDKTY HYYSLPLAAK SLGDITRLPK SLKVLLENLL RWQDGNSVTE EDIHALAGWL KNAHADREIA YRPARVLMQD FTGVPAVVDL AAMREAVKRL GGDTAKVNPL SPVDLVIDHS VTVDRFGDDE AFEENVRLEM ERNHERYVFL KWGKQAFSRF SVVPPGTGIC HQVNLEYLGK AVWSELQDGE WIAYPDTLVG TDSHTTMING LGVLGWGVGG IEAEAAMLGQ PVSMLIPDVV GFKLTGKLRE GITATDLVLT VTQMLRKHGV VGKFVEFYGD GLDSLPLADR ATIANMSPEY GATCGFFPID AVTLDYMRLS GRSEDQVELV EKYAKAQGMW RNPGDEPIFT STLELDMNDV EASLAGPKRP QDRVALPDVP KAFAASNELE VNATHKDRQP VDYVMNGHQY QLPDGAVVIA AITSCTNTSN PSVLMAAGLL AKKAVTLGLK RQPWVKASLA PGSKVVSDYL AKAKLTPYLD ELGFNLVGYG CTTCIGNSGP LPDPIETAIK KSDLTVGAVL SGNRNFEGRI HPLVKTNWLA SPPLVVAYAL AGNMNINLAS EPIGHDRKGD PVYLKDIWPS AQEIARAVEQ VSTEMFRKEY AEVFEGTAEW KGINVTRSDT YGWQEDSTYI RLSPFFDEMQ ATPAPVEDIH GARILAMLGD SVTTDHISPA GSIKPDSPAG RYLQGRGVER KDFNSYGSRR GNHEVMMRGT FANIRIRNEM VPGVEGGMTR HLPDSDVVSI YDAAMRYKQE QTPLAVIAGK EYGSGSSRDW AAKGPRLLGI RVVIAESFER IHRSNLIGMG ILPLEFPQGV TRKTLGLTGE EKIDIGDLQN LQPGATVPVT LTRADGSQEV VPCRCRIDTA TELTYYQNDG ILHYVIRNML K |
Length |
891 |
Mol. Wt |
97.678 kDa |
pI |
5.8 (calculated) |
Extinction coefficient |
114,710 - 115,585 (calc based on 29 Y, 13 W, and 7 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
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Resource type | Source | Notes/Reference |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Prodromou, C et al. (1991) The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn). J. Gen. Microbiol. 137 2505-15 PubMed
- ↑ 5.0 5.1 5.2 Tang, Y & Guest, JR (1999) Direct evidence for mRNA binding and post-transcriptional regulation by Escherichia coli aconitases. Microbiology (Reading, Engl.) 145 ( Pt 11) 3069-79 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 Varghese, S et al. (2003) Contrasting sensitivities of Escherichia coli aconitases A and B to oxidation and iron depletion. J. Bacteriol. 185 221-30 PubMed
- ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 8.0 8.1 8.2 8.3 8.4 8.5 8.6 8.7 8.8 8.9 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005737 ! cytoplasm
- GO:0003994 ! aconitate hydratase activity
- GO:0005506 ! iron ion binding
- GO:0006099 ! tricarboxylic acid cycle
- GO:0051539 ! 4 iron, 4 sulfur cluster binding
- GO:0003729 ! mRNA binding
- GO:0006097 ! glyoxylate cycle
- GO:0046872 ! metal ion binding
- GO:0006979 ! response to oxidative stress
- GO:0009061 ! anaerobic respiration
- Proteins
- RefGenome Annotated Gene