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Standard Name	accB
Gene Synonym(s)	ECK3242, b3255, JW3223, fabE^[1], fabE
Product Desc.	biotinylated biotin-carboxyl carrier protein^[2]^[3], carboxybiotin-carboxyl-carrier protein^[2]^[3]; Component of AccB transcriptional repressor; biotin carboxyl carrier protein (dimer)^[2]^[3]; acetyl CoA carboxylase^[2]^[3] Acetyl-CoA carboxylase, biotin carboxyl carrier protein; BCCP; homodimeric^[4]
Product Synonyms(s)	acetyl CoA carboxylase, BCCP subunit^[1], B3255^[2]^[1], FabE^[2]^[1], [acetyl-CoA:carbon-dioxide ligase (ADP forming)]^[2]^[1], BCCP-biotin (monomer)^[2]^[1], BCCP-biotin-CO2^[2], biotin-BCCP (monomer)^[2]^[1] , ECK3242, fabE, JW3223, b3255
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): accBC^[2]
Regulation/Activity
Quick Links
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Notes

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AccB is biotinylated on K122 by BirA.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	accB
Mnemonic	Acetyl-CoA carboxylase
Synonyms	ECK3242, b3255, JW3223, fabE^[1], fabE
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.36 minutes	MG1655: 3403458..3403928 <gbrowseImage> name=NC_000913:3403458..3403928 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3333327..3333797 <gbrowseImage> name=NC_012967:3333327..3333797 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3290606..3291076 <gbrowseImage> name=NC_012759:3290606..3291076 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3405291..3405761 <gbrowseImage> name=NC_007779:3405291..3405761 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3501203..3501673 <gbrowseImage> name=NC_010473:3501203..3501673 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3403458	Edman degradation	PMID:1370469 PMID:9740056
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Phenotype: Type	Phenotype: Description	Reference	Availability
accB22(ts)		temperature sensitive		CGSC:9109
accB⁺⁺	Overexpression	Increase Acetyle COA Carboxylase activity upon over expression.	PMID:10893421	See Table 1.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3223	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATATTCGTAAGATTAAAAA Primer 2:CCtTCGATGACGACCAGCGGCTC
21D3	Kohara Phage	Genobase	PMID:3038334
6G3	Kohara Phage	Genobase	PMID:3038334
zhc-6::Tn10	Linked marker	CAG12153 = CGSC7442^[5]	est. P1 cotransduction: 19% ^[6] Synonyms:zha-6::Tn10
acrF3083::Tn10	Linked marker	CAG12075 = CGSC7447^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:zhd-3083::Tn10, zhe-3083::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10275	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10275	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000269	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947758	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0271	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010675	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	AccB
Synonyms	acetyl CoA carboxylase, BCCP subunit^[1], B3255^[2]^[1], FabE^[2]^[1], [acetyl-CoA:carbon-dioxide ligase (ADP forming)]^[2]^[1], BCCP-biotin (monomer)^[2]^[1], BCCP-biotin-CO2^[2], biotin-BCCP (monomer)^[2]^[1] , ECK3242, fabE, JW3223, b3255
Product description	biotinylated biotin-carboxyl carrier protein^[2]^[3], carboxybiotin-carboxyl-carrier protein^[2]^[3]; Component of AccB transcriptional repressor; biotin carboxyl carrier protein (dimer)^[2]^[3]; acetyl CoA carboxylase^[2]^[3] Acetyl-CoA carboxylase, biotin carboxyl carrier protein; BCCP; homodimeric^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003989	acetyl-CoA carboxylase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001249	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001249	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0275	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	PMID:10893421	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0009317	acetyl-CoA carboxylase complex	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001249	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009374	biotin binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001882	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006633	fatty acid biosynthetic process	PMID:10893421	IGI: Inferred from Genetic Interaction	EcoliWiki:accA EcoliWiki:accC EcoliWiki:accD	P		complete
	GO:0003989	acetyl-CoA carboxylase activity	PMID:10893421	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0005737	cytoplasm	PMID:10893421	IC: Inferred by Curator	GO:0003989	C		complete
	GO:0006633	fatty acid biosynthetic process	PMID:10893421	IMP: Inferred from Mutant Phenotype		P	Fig 3: TLC showing excess FFA production in mutants over-expressing ACC. Table II shows relative FFA production higher in mutants over-expressing ACC than controls.	complete
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Interactions

<protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	gltX		PMID:16606699	Experiment(s):EBI-1145305
Protein	rpsC		PMID:16606699	Experiment(s):EBI-1145305
Protein	accC		PMID:16606699	Experiment(s):EBI-1145305, EBI-1145313
Protein	ykgF		PMID:16606699	Experiment(s):EBI-1145305
Protein	pmbA		PMID:16606699	Experiment(s):EBI-1145305
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1145305
Protein	Subunits of AccB transcriptional repressor; biotin carboxyl carrier protein (dimer)	could be indirect
Protein	Subunits of acetyl CoA carboxylase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDIRKIKKLI ELVEESGISE LEISEGEESV RISRAAPAAS FPVMQQAYAA PMMQQPAQSN AAAPATVPSM EAPAAAEISG HIVRSPMVGT FYRTPSPDAK AFIEVGQKVN VGDTLCIVEA MKMMNQIEAD KSGTVKAILV ESGQPVEFDE PLVVIE
Length	156
Mol. Wt	16.686 kDa
pI	4.5 (calculated)
Extinction coefficient	2,980 - 3,105 (calc based on 2 Y, 0 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	81..155	PF00364 Biotin-requiring enzyme		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=accB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131143	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947758	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010675	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABD8	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10275	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10275	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947758	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000269	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0271	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.32E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	34495	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	5637	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	13718	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
accBC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3403438..3403478 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3255 (EcoliWiki Page)	NCBI GEO profiles for accB
microarray	GenExpDB:b3255 (EcoliWiki Page)	Summary of data for accB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3402156..3403121) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:B8^[7]
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Notes

Accessions Related to accB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10275	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0271	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3255
EcoGene	EcoGene:EG10275	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000269	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010675	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT5G16390 (score: 1.000; bootstrap: 99%) AT5G15530 (score: 0.241)	From Inparanoid:20070104
Shigella flexneri	ACCB	From SHIGELLACYC
E. coli O157	ACCB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00364 Biotin-requiring enzyme
Superfamily (EcoliWiki Page)	SUPERFAMILY:51230
Panther (EcoliWiki Page)	PTHR18866:SF59
EcoCyc	EcoCyc:EG10275	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10275	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000269	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0271	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010675	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 ^2.19 ^2.20 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

accB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to accB Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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