accB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

accB

Mnemonic

Acetyl-CoA carboxylase

Synonyms

ECK3242, b3255, JW3223, fabE[1], fabE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

73.36 minutes 

MG1655: 3403458..3403928
<gbrowseImage> name=NC_000913:3403458..3403928 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3333327..3333797
<gbrowseImage> name=NC_012967:3333327..3333797 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3290606..3291076
<gbrowseImage> name=NC_012759:3290606..3291076 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3405291..3405761
<gbrowseImage> name=NC_007779:3405291..3405761 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3501203..3501673
<gbrowseImage> name=NC_010473:3501203..3501673 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3403458

Edman degradation

PMID:1370469[2]
PMID:9740056[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

accB22(ts)

temperature sensitive

CGSC:9109

accB ++

Overexpression

Increase Acetyle COA Carboxylase activity upon over expression.

PMID:10893421[4]

See Table 1.


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3223

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGATATTCGTAAGATTAAAAA

Primer 2:CCtTCGATGACGACCAGCGGCTC

21D3

Kohara Phage

Genobase

PMID:3038334[6]

6G3

Kohara Phage

Genobase

PMID:3038334[6]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[7]

est. P1 cotransduction: 19% [8]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[7]

est. P1 cotransduction: 68% [8]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10275

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10275

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000269

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947758

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0271

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010675

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Li, SJ & Cronan, JE Jr (1992) The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase. J. Biol. Chem. 267 855-63 PubMed
  3. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  4. Davis, MS et al. (2000) Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. J. Biol. Chem. 275 28593-8 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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