gpsA:On One Page
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | |
Quickview
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| Standard Name |
gpsA |
|---|---|
| Gene Synonym(s) |
ECK3598, b3608, JW3583[1], JW3583 |
| Product Desc. |
Component of [[:Category:Complex:glycerol-3-phosphate-dehydrogenase-[NAD+]|glycerol-3-phosphate-dehydrogenase-[NAD+]]][2][3] |
| Product Synonyms(s) |
glycerol-3-phosphate dehydrogenase (NAD+)[1], B3608[2][1], GpsA[2][1] , ECK3598, JW3583, b3608 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links | |
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
gpsA |
|---|---|
| Mnemonic |
Glycerol phosphate |
| Synonyms |
ECK3598, b3608, JW3583[1], JW3583 |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
81.49 minutes |
MG1655: 3781684..3780665 |
||
|
NC_012967: 3724427..3723408 |
||||
|
NC_012759: 3668991..3670010 |
||||
|
W3110 |
|
W3110: 3856754..3857773 |
||
|
DH10B: 3879261..3878242 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
gpsA::Tn5KAN-I-SceI (FB21340) |
Insertion at nt 476 in Plus orientation |
PMID:15262929 |
contains pKD46 | ||||
|
gpsA20 |
| ||||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3583 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAACCAACGTAATGCTTCAAT Primer 2:CCGTGGCTGCTGCGCTCGTCCTT | |
|
Kohara Phage |
PMID:3038334 | ||
|
zhg-50::Tn10 |
Linked marker |
est. P1 cotransduction: % [5] | |
|
zic-4901::Tn10 |
Linked marker |
est. P1 cotransduction: 12% [5] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002442 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EchoBASE:EB4142 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011795 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
GpsA |
|---|---|
| Synonyms |
glycerol-3-phosphate dehydrogenase (NAD+)[1], B3608[2][1], GpsA[2][1] , ECK3598, JW3583, b3608 |
| Product description |
Component of [[:Category:Complex:glycerol-3-phosphate-dehydrogenase-[NAD+]|glycerol-3-phosphate-dehydrogenase-[NAD+]]][2][3] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003824 |
catalytic activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004367 |
glycerol-3-phosphate dehydrogenase (NAD+) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005488 |
binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005975 |
carbohydrate metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006072 |
glycerol-3-phosphate metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008152 |
metabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008654 |
phospholipid biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008654 |
phospholipid biosynthetic process |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009331 |
glycerol-3-phosphate dehydrogenase complex |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016614 |
oxidoreductase activity, acting on CH-OH group of donors |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016616 |
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046474 |
glycerophospholipid biosynthetic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0046167 |
glycerol-3-phosphate biosynthetic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0047952 |
glycerol-3-phosphate dehydrogenase (NAD(P)+) activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0046168 |
glycerol-3-phosphate catabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0047952 |
glycerol-3-phosphate dehydrogenase (NAD(P)+) activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0047952 |
glycerol-3-phosphate dehydrogenase (NAD(P)+) activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0050662 |
coenzyme binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051287 |
NAD or NADH binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055114 |
oxidation reduction |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0055114 |
oxidation reduction |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of [[:Category:Complex:glycerol-3-phosphate-dehydrogenase-[NAD+]|glycerol-3-phosphate-dehydrogenase-[NAD+]]] |
could be indirect |
||
|
Protein |
cadA |
Experiment(s):EBI-1146237 | ||
|
Protein |
ahpC |
Experiment(s):EBI-1146237 | ||
|
Protein |
rho |
Experiment(s):EBI-1146237 | ||
|
Protein |
speG |
Experiment(s):EBI-1146237 | ||
|
Protein |
nadE |
Experiment(s):EBI-1146237 | ||
|
Protein |
rpsA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplW |
LCMS(ID Probability):99.6 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MNQRNASMTV IGAGSYGTAL AITLARNGHE VVLWGHDPEH IATLERDRCN AAFLPDVPFP DTLHLESDLA TALAASRNIL VVVPSHVFGE VLRQIKPLMR PDARLVWATK GLEAETGRLL QDVAREALGD QIPLAVISGP TFAKELAAGL PTAISLASTD QTFADDLQQL LHCGKSFRVY SNPDFIGVQL GGAVKNVIAI GAGMSDGIGF GANARTALIT RGLAEMSRLG AALGADPATF MGMAGLGDLV LTCTDNQSRN RRFGMMLGQG MDVQSAQEKI GQVVEGYRNT KEVRELAHRF GVEMPITEEI YQVLYCGKNA REAALTLLGR ARKDERSSH |
| Length |
339 |
| Mol. Wt |
36.361 kDa |
| pI |
6.5 (calculated) |
| Extinction coefficient |
18,450 - 18,950 (calc based on 5 Y, 2 W, and 4 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
ASAP:ABE-0011795 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EcoCyc:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB:ECK120002442 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB4142 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
E. coli K-12 MC4100 |
1.86E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
|
Protein |
Ecoli K-12 |
26.034+/-0.229 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
|
mRNA |
Ecoli K-12 |
0.158488714 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
|
Protein |
E. coli K-12 MG1655 |
1471 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
355 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
602 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
| edit table |
Notes
Transcription and Transcriptional Regulation
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<gbrowseImage> name=NC_000913:3781664..3781704 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for gpsA | |
|
microarray |
Summary of data for gpsA from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
| edit table |
<protect>
Notes
Accessions Related to gpsA Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB4142 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002442 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011795 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Anopheles gambiae |
|
From Inparanoid:20070104 |
|
Apis mellifera |
|
From Inparanoid:20070104 |
|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
|
Bos taurus |
|
From Inparanoid:20070104 |
|
Caenorhabditis briggsae |
|
From Inparanoid:20070104 |
|
Caenorhabditis elegans |
|
From Inparanoid:20070104 |
|
Canis familiaris |
|
From Inparanoid:20070104 |
|
Ciona intestinalis |
|
From Inparanoid:20070104 |
|
Danio rerio |
|
From Inparanoid:20070104 |
|
Drosophila melanogaster |
|
From Inparanoid:20070104 |
|
Drosophila pseudoobscura |
|
From Inparanoid:20070104 |
|
Gallus gallus |
|
From Inparanoid:20070104 |
|
Homo sapiens |
|
From Inparanoid:20070104 |
|
Macaca mulatta |
|
From Inparanoid:20070104 |
|
Monodelphis domestica |
|
From Inparanoid:20070104 |
|
Mus musculus |
|
From Inparanoid:20070104 |
|
Pan troglodytes |
|
From Inparanoid:20070104 |
|
Rattus norvegicus |
|
From Inparanoid:20070104 |
|
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
|
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
|
Takifugu rubripes |
|
From Inparanoid:20070104 |
|
Tetraodon nigroviridis |
|
From Inparanoid:20070104 |
|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
|
Shigella flexneri |
GPSA |
From SHIGELLACYC |
|
E. coli O157 |
GPSA |
From ECOO157CYC |
| edit table |
Do-It-Yourself Web Tools
Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
PF01210 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus |
||
|
PF07479 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus |
||
|
EcoCyc:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG20091 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120002442 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB4142 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0011795 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 6.0 6.1 Cronan, JE Jr & Bell, RM (1974) Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J. Bacteriol. 118 598-605 PubMed
- ↑ 7.0 7.1 7.2 7.3 7.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 8.0 8.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003824 ! catalytic activity
- GO:0004367 ! glycerol-3-phosphate dehydrogenase (NAD+) activity
- GO:0005488 ! binding
- GO:0005737 ! cytoplasm
- GO:0005975 ! carbohydrate metabolic process
- GO:0006072 ! glycerol-3-phosphate metabolic process
- GO:0008152 ! metabolic process
- GO:0008654 ! phospholipid biosynthetic process
- GO:0009331 ! glycerol-3-phosphate dehydrogenase complex
- GO:0016614 ! oxidoreductase activity, acting on CH-OH group of donors
- GO:0016616 ! oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
- GO:0046474 ! glycerophospholipid biosynthetic process
- GO:0046167 ! glycerol-3-phosphate biosynthetic process
- GO:0047952 ! glycerol-3-phosphate dehydrogenase (NAD(P)+) activity
- GO:0046168 ! glycerol-3-phosphate catabolic process
- GO:0050662 ! coenzyme binding
- GO:0051287 ! NAD binding
- GO:0055114 ! oxidation-reduction process
- Genes with homologs in Anopheles gambiae
- Genes with homologs in Apis mellifera
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Bos taurus
- Genes with homologs in Caenorhabditis briggsae
- Genes with homologs in Caenorhabditis elegans
- Genes with homologs in Canis familiaris
- Genes with homologs in Ciona intestinalis
- Genes with homologs in Danio rerio
- Genes with homologs in Drosophila melanogaster
- Genes with homologs in Drosophila pseudoobscura
- Genes with homologs in Gallus gallus
- Genes with homologs in Homo sapiens
- Genes with homologs in Macaca mulatta
- Genes with homologs in Monodelphis domestica
- Genes with homologs in Mus musculus
- Genes with homologs in Pan troglodytes
- Genes with homologs in Rattus norvegicus
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Takifugu rubripes
- Genes with homologs in Tetraodon nigroviridis
- Genes with homologs in Xenopus tropicalis
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157



