gpsA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GpsA

Synonyms

glycerol-3-phosphate dehydrogenase (NAD+)[1], B3608[2][1], GpsA[2][1] , ECK3598, JW3583, b3608

Product description

GpsA[2][3];

Component of [[:Category:Complex:glycerol-3-phosphate-dehydrogenase-[NAD+]|glycerol-3-phosphate-dehydrogenase-[NAD+]]][2][3]

EC number (for enzymes)

<protect></protect>

Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0004367

glycerol-3-phosphate dehydrogenase (NAD+) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006168

F

Seeded from EcoCyc (v14.0)

complete

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00394

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011128

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006109

P

Seeded from EcoCyc (v14.0)

complete

GO:0006072

glycerol-3-phosphate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006168

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc (v14.0)

complete

GO:0008654

phospholipid biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00394

P

Seeded from EcoCyc (v14.0)

complete

GO:0008654

phospholipid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0594

P

Seeded from EcoCyc (v14.0)

complete

GO:0009331

glycerol-3-phosphate dehydrogenase complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006168

C

Seeded from EcoCyc (v14.0)

complete

GO:0016614

oxidoreductase activity, acting on CH-OH group of donors

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006109

F

Seeded from EcoCyc (v14.0)

complete

GO:0016616

oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011128

F

Seeded from EcoCyc (v14.0)

complete

GO:0046474

glycerophospholipid biosynthetic process

PMID:4597451[4]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0046167

glycerol-3-phosphate biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00394

P

Seeded from EcoCyc (v14.0)

complete

GO:0047952

glycerol-3-phosphate dehydrogenase (NAD(P)+) activity

PMID:4597451[4]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0046168

glycerol-3-phosphate catabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011128

P

Seeded from EcoCyc (v14.0)

complete

GO:0047952

glycerol-3-phosphate dehydrogenase (NAD(P)+) activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00394

F

Seeded from EcoCyc (v14.0)

complete

GO:0047952

glycerol-3-phosphate dehydrogenase (NAD(P)+) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.94

F

Seeded from EcoCyc (v14.0)

complete

GO:0050662

coenzyme binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013328

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011128

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006109

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006168

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011128

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of [[:Category:Complex:glycerol-3-phosphate-dehydrogenase-[NAD+]|glycerol-3-phosphate-dehydrogenase-[NAD+]]]

could be indirect

Protein

cadA

PMID:16606699[5]

Experiment(s):EBI-1146237

Protein

ahpC

PMID:16606699[5]

Experiment(s):EBI-1146237

Protein

rho

PMID:16606699[5]

Experiment(s):EBI-1146237

Protein

speG

PMID:16606699[5]

Experiment(s):EBI-1146237

Protein

nadE

PMID:16606699[5]

Experiment(s):EBI-1146237

Protein

rpsA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rplW

PMID:19402753[6]

LCMS(ID Probability):99.6

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MNQRNASMTV IGAGSYGTAL AITLARNGHE VVLWGHDPEH IATLERDRCN AAFLPDVPFP
DTLHLESDLA TALAASRNIL VVVPSHVFGE VLRQIKPLMR PDARLVWATK GLEAETGRLL
QDVAREALGD QIPLAVISGP TFAKELAAGL PTAISLASTD QTFADDLQQL LHCGKSFRVY
SNPDFIGVQL GGAVKNVIAI GAGMSDGIGF GANARTALIT RGLAEMSRLG AALGADPATF
MGMAGLGDLV LTCTDNQSRN RRFGMMLGQG MDVQSAQEKI GQVVEGYRNT KEVRELAHRF
GVEMPITEEI YQVLYCGKNA REAALTLLGR ARKDERSSH
Length

339

Mol. Wt

36.361 kDa

pI

6.5 (calculated)

Extinction coefficient

18,450 - 18,950 (calc based on 5 Y, 2 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..165

PF01210 NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus

PMID:19920124[7]

Domain

183..327

PF07479 NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus

PMID:19920124[7]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gpsA taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131479

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948125

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011795

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6S7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG20091

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20091

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948125

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002442

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4142

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 Cronan, JE Jr & Bell, RM (1974) Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J. Bacteriol. 118 598-605 PubMed
  5. 5.0 5.1 5.2 5.3 5.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. 6.0 6.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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