PMID:4597451
| Citation |
Cronan, JE Jr and Bell, RM (1974) Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of the structural gene for L-glycerol 3-phosphate dehydrogenase. J. Bacteriol. 118:598-605 |
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| Abstract |
The structural gene for the biosynthetic l-glycerol 3-phosphate dehydrogenase has been mapped at min 71.5 on the Escherichia coli chromosome. This gene (gpsA) is co-transduced with the xyl, mtl, and pyrE loci. Three-factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosone is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature-sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase-deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful. |
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| Keywords |
Cell Membrane/metabolism; Chromosome Mapping; Conjugation, Genetic; Crosses, Genetic; Escherichia coli/enzymology; Escherichia coli/metabolism; Genes; Genetic Complementation Test; Glycerolphosphate Dehydrogenase/biosynthesis; Glycerophosphates/metabolism; Mutation; Phospholipids/biosynthesis; Recombination, Genetic; Spectrophotometry; Stereoisomerism; Temperature; Transduction, Genetic |
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