trxA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

trxA

Mnemonic

Thioredoxin

Synonyms

ECK3773, b3781, JW5856, fipA, tsnC, dasC[1], dasC


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

85.43 minutes, 85.43 minutes 

MG1655: 3963784..3964113
<gbrowseImage> name=NC_000913:3963784..3964113 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3927561..3927890
<gbrowseImage> name=NC_012967:3927561..3927890 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3853453..3853782
<gbrowseImage> name=NC_012759:3853453..3853782 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3670920..3670591
<gbrowseImage> name=AC_000091:3670591..3670920 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4062704..4063033
<gbrowseImage> name=NC_010473:4062704..4063033 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3963787

Edman degradation

PMID:4883076[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtrxA (Keio:JW5856)

deletion

deletion

PMID:16738554[3]

Shigen

trxA1(Am)

PMID:337124[4] PMID:3881756[5]

CGSC:5031

amber (UAG) mutation

ΔtrxA307

CGSC:10957

trxA11(ts)

temperature sensitive

PMID:3881756[5]

CGSC:88597



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5856

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAGCGATAAAATTATTCACCT

Primer 2:CCtGCCAGGTTAGCGTCGAGGAA

7B1

Kohara Phage

Genobase

PMID:3038334[7]

2A8

Kohara Phage

Genobase

PMID:3038334[7]

ilvD500::Tn10

Linked marker

CAG18431 = CGSC7462[8]

est. P1 cotransduction: 69% [9]
Synonyms:ilv500::Tn10

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[8]

est. P1 cotransduction: 14% [9]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG11031

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG11031

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948289

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Holmgren, A (1968) Thioredoxin. 6. The amino acid sequence of the protein from escherichia coli B. Eur. J. Biochem. 6 475-84 PubMed
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Mark, DF et al. (1977) Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol. Gen. Genet. 155 145-52 PubMed
  5. ↑ 5.0 5.1 Russel, M & Model, P (1985) Thioredoxin is required for filamentous phage assembly. Proc. Natl. Acad. Sci. U.S.A. 82 29-33 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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