PMID:3881756
| Citation |
Russel, M and Model, P (1985) Thioredoxin is required for filamentous phage assembly. Proc. Natl. Acad. Sci. U.S.A. 82:29-33 |
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| Abstract |
Sequence comparisons show that the fip gene product of Escherichia coli, which is required for filamentous phage assembly, is thioredoxin. Thioredoxin serves as a cofactor for reductive processes in many cell types and is a constituent of phage T7 DNA polymerase. The fip-1 mutation makes filamentous phage and T7 growth temperature sensitive in cells that carry it. The lesion lies within a highly conserved thioredoxin active site. Thioredoxin reductase (NADPH), as well as thioredoxin, is required for efficient filamentous phage production. Mutant phages defective in phage gene I are particularly sensitive to perturbations in the fip-thioredoxin system. A speculative model is presented in which thioredoxin reductase, thioredoxin, and the gene I protein interact to drive an engine for filamentous phage assembly. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/physiology; Base Sequence; Coliphages/growth & development; Escherichia coli/genetics; Genes, Bacterial; Morphogenesis; Mutation; Thioredoxins/genetics; Thioredoxins/physiology; Virus Replication |
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