proA:Gene: Difference between revisions

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Revision as of 23:45, 8 November 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

proA

Mnemonic
Synonyms

ECK0244, b0243, JW0233, pro1, pro(1)[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.62 minutes 

MG1655: 260727..261980
gbrowse_img.php?coord=259727..262980&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 260727..261980
gbrowse_img.php?coord=259727..262980&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔproA (Keio:JW0233)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8468[3]

proA81::Tn10

Insertion at 261,286 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG18447 = CGSC7330[3]

Synonyms:

proA761(del)::kan

insertion

insertion

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW0233

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCTGGAACAAATGGGCATTGC

Primer 2:CCCGCACGAATGGTGTAATCACC

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[3]

est. P1 cotransduction: 33% [4]

Synonyms:zae-502::Tn10

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[3]

est. P1 cotransduction: % [4]

Synonyms:lacI42::Tn10


CAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10767

EchoBASE (EcoliWiki Page)

EchoBASE:EB0760

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0243

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0390

EcoGene (EcoliWiki Page)

EcoGene:EG10767

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PROA

CGSC (EcoliWiki Page)

CGSC:364

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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed


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