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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10317 ECOCYC:EG10317]
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10317 EcoGene:EG10317]
EcoGene:EG10317
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Escherichia coli str. K-12 substr. MG1655  
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RegulonDB:ECK120000311
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB0313
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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ASAP:ABE-0010703
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:29, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fis

Mnemonic

Factor for inversion stimulation

Synonyms

ECK3248, b3261, JW3229, nbp[1], nbp

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

73.48 minutes 

MG1655: 3409293..3409589
<gbrowseImage> name=NC_000913:3409293..3409589 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3339162..3339458
<gbrowseImage> name=NC_012967:3339162..3339458 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3296441..3296737
<gbrowseImage> name=NC_012759:3296441..3296737 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3411126..3411422
<gbrowseImage> name=AC_000091:3411126..3411422 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3507038..3507334
<gbrowseImage> name=NC_010473:3507038..3507334 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3409293

Edman degradation

PMID:2835774[2]
PMID:2837762[3]
PMID:8809757[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δfis (Keio:JW3229)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC10443[6]

Δfis::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938[7]

Δfis-779::kan

PMID:16738554[5]

CGSC:100880

fis(del)-767::khan

Growth Phenotype

Growth rate regulation of the amount of the pdxA-ksgA contranscript was abolished by mutation, figure 7.

PMID:11844765[8]

Strain: TX3333

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3229

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCTTCGAACAACGCGTAAATTC

Primer 2:CCGTTCATGCCGTATTTTTTCAA

6G3

Kohara Phage

Genobase

PMID:3038334[10]

6G9

Kohara Phage

Genobase

PMID:3038334[10]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[6]

est. P1 cotransduction: 13% [11]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[6]

est. P1 cotransduction: 83% [11]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10317

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10317

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947697

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000311

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0313

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010703

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Johnson, RC et al. (1988) Isolation of the gene encoding the Hin recombinational enhancer binding protein. Proc. Natl. Acad. Sci. U.S.A. 85 3484-8 PubMed
  3. ↑ Koch, C et al. (1988) Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene. Proc. Natl. Acad. Sci. U.S.A. 85 4237-41 PubMed
  4. ↑ Green, J et al. (1996) The ndh-binding protein (Nbp) regulates the ndh gene of Escherichia coli in response to growth phase and is identical to Fis. Mol. Microbiol. 20 1043-55 PubMed
  5. ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  8. ↑ Pease, AJ et al. (2002) Positive growth rate-dependent regulation of the pdxA, ksgA, and pdxB genes of Escherichia coli K-12. J. Bacteriol. 184 1359-69 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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