PMID:2837762
| Citation |
Koch, C, Vandekerckhove, J and Kahmann, R (1988) Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene. Proc. Natl. Acad. Sci. U.S.A. 85:4237-41 |
|---|---|
| Abstract |
The Escherichia coli (Es. coli) protein Fis (factor for inversion stimulation) stimulates site-specific DNA inversion of the G segment in phage Mu by binding to a recombinational enhancer. By using synthetic oligonucleotides deduced from the amino-terminal amino acid sequence, we have cloned the gene (termed fis) encoding this specific DNA-binding protein. The DNA sequence shows that the Fis protein is basic and contains 98 amino acids. A helix-turn-helix sequence motif characteristic of many DNA-binding proteins is located at the carboxyl-terminal end of the protein. By marker exchange, we have constructed an insertion mutation of fis. Fis is nonessential for Es. coli growth; however, inversion of the G segment of a Mu prophage was not detected in the fis mutant. The fis gene is located between 71 and 72 min on the Es. coli genetic map. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Carrier Proteins/genetics; Chromosome Inversion; Cloning, Molecular; DNA Restriction Enzymes; DNA, Bacterial/genetics; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli Proteins; Factor For Inversion Stimulation Protein; Genes; Genes, Bacterial; Integration Host Factors; Molecular Sequence Data |
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