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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10241 ECOCYC:EG10241]
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10241 EcoGene:EG10241]
EcoGene:EG10241
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Escherichia coli str. K-12 substr. MG1655  
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RegulonDB:ECK120000235
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB0237
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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ASAP:ABE-0000052
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:18, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaK

Mnemonic

DNA

Synonyms

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.26 minutes 

MG1655: 12163..14079
<gbrowseImage> name=NC_000913:12163..14079 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 12161..14077
<gbrowseImage> name=NC_012967:12161..14077 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 12163..14079
<gbrowseImage> name=NC_012759:12163..14079 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 12163..14079
<gbrowseImage> name=AC_000091:12163..14079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 12163..14079
<gbrowseImage> name=NC_010473:12163..14079 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

12166

Edman degradation

PMID:1396676[3]
PMID:7783627[4]
PMID:9298646[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dnaK(del) (Keio:JW0013)

deletion

deletion

PMID:16738554[6]

Shigen

CGSC8342[7]

dnaKG32D

G32D

In SEG-1 and dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKV436I

V436I

In mutant SEG-2; confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG455D

G455D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG468D

G468D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaK756(ts)

temperature sensitive

CGSC:6707

dnaK(del)734::kan

deletion

deletion

PMID:16738554[6]

CGSC:100628

dnaK

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484[8]

fig 2

DnaKG384A

Glycine to Alanine at position 384.

There is a 13 fold decrease rate for substrate release in ATP bound state and increase in substrate affinity. with an overall 2.5 fold higher chaperon activity, Table 1.

PMID:21656889[9]

G384A/Cd35

Double mutant

The double mutant combines accelerated peptide binding in its ADP-bound state (like Cd35) and slower peptide release in its ATP-bound state (likeG384A), but without additivity in DnaK-assisted firefly luciferase refolding, table 1.

PMID:21656889[9]

Cd35

35-residue C-terminal truncation

Cd35 showed a 7-fold increase in initial refolding rate for denatured luciferase and resulted in a 5-fold increase in maximal luminescence as compared to wild type DnaK, Table 3.

PMID:20381501[10]

dnaK7

revertant

Mutant is Leu-. When Leu-auxotrophs of Escherichia coli after UV irradiation, were grown at temperatures between 30 and 47C the frequent of UV-induced mutation from Leu- to Leu+ increased as UV dose and temperature increased. Figure 2.

PMID:22270474[11]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0013

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCGGTAAAATAATTGGTATCGA

Primer 2:CCTTTTTTGTCTTTGACTTCTTC

9E4

Kohara Phage

Genobase

PMID:3038334[13]

6H3

Kohara Phage

Genobase

PMID:3038334[13]

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[7]

est. P1 cotransduction: 66% [14]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[7]

est. P1 cotransduction: 48% [14]
Synonyms:car-96::Tn10

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Notes

Sugimoto et al [15] constructed a strain carrying a dnaK deletion and the lambda DE3 prophage, which expresses T7 RNA polymerase under lac control.

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944750

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Schmid, D et al. (1992) Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat-shock protein DnaK. Eur. J. Biochem. 208 699-704 PubMed
  4. ↑ Freestone, P et al. (1995) Identification of phosphoproteins in Escherichia coli. Mol. Microbiol. 15 573-80 PubMed
  5. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. ↑ 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  9. ↑ 9.0 9.1 Schweizer, RS et al. (2011) Fine tuning of a biological machine: DnaK gains improved chaperone activity by altered allosteric communication and substrate binding. Chembiochem 12 1559-73 PubMed
  10. ↑ Aponte, RA et al. (2010) Directed evolution of the DnaK chaperone: mutations in the lid domain result in enhanced chaperone activity. J. Mol. Biol. 399 154-67 PubMed
  11. ↑ Saha, S & Basu, T (2012) Increase in UV mutagenesis by heat stress on UV-irradiated E. coli cells. Biochem. Genet. 50 484-93 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  15. ↑ Sugimoto, S et al. (2008) Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins. Protein Expr. Purif. 60 31-6 PubMed

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