PMID:22270474
| Citation |
Saha, S and Basu, T (2012) Increase in UV mutagenesis by heat stress on UV-irradiated E. coli cells. Biochem. Genet. 50:484-93 |
|---|---|
| Abstract |
When leu- auxotrophs of Escherichia coli, after UV irradiation, were grown at temperatures between 30 and 47°C, the frequency of UV-induced mutation from leu- to leu+ revertant increased as the UV dose and the temperature increased. For cells exposed to a UV dose of 45 J/m2, the mutation frequency at 47°C was 1.9 times that at 30°C; for a dose of 90 J/m2, it was 3.25 times; and for 135 J/m2, it was 4.8 times. Similar enhancement of reversion frequency was observed when the irradiated cells were grown at 30°C in the presence of a heat shock inducer, ethanol (8% v/v). Heat shock-mediated enhancement of UV mutagenesis did not occur in an E. coli mutant sigma 32 (heat shock regulator protein), but sigma 32 overexpression in the mutant strain (transformed with a sigma 32-bearing plasmid) increased the UV-induced mutation frequency. These results suggest that heat stress alone has no mutagenic property, but when applied to UV-damaged cells, it enhances the UV-induced frequency of cell mutation. |
| Links |
PubMed Online version:10.1007/s10528-011-9493-x |
| Keywords |
DNA Repair; Escherichia coli/genetics; Escherichia coli/radiation effects; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Heat-Shock Response/genetics; Mutagenesis; Mutation; Sigma Factor/genetics; Sigma Factor/metabolism; Temperature; Ultraviolet Rays/adverse effects |
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