trxA:Gene: Difference between revisions
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=555 Genobase] | [http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=555 Genobase] | ||
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PMID:3038334 | |||
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[[:Category:Kohara_Phage:2A8|2A8]] | |||
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Kohara Phage | |||
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=556 Genobase] | |||
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PMID:3038334 | PMID:3038334 | ||
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est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/> <br /> | est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype. | Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype. | ||
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Revision as of 21:42, 21 September 2011
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
trxA |
|---|---|
| Mnemonic |
Thioredoxin |
| Synonyms |
ECK3773, b3781, JW5856, fipA, tsnC, dasC[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
85.43 minutes, 85.43 minutes |
MG1655: 3963784..3964113 |
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NC_012967: 3927561..3927890 |
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NC_012759: 3853453..3853782 |
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W3110 |
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W3110: 3670920..3670591 |
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DH10B: 4062704..4063033 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3963787 |
Edman degradation |
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| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔtrxA (Keio:JW5856) |
deletion |
deletion |
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trxA1(Am) |
amber (UAG) mutation | ||||||
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ΔtrxA307 |
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|
trxA11(ts) |
temperature sensitive |
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| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5856 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGCGATAAAATTATTCACCT Primer 2:CCtGCCAGGTTAGCGTCGAGGAA | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 69% [9] | ||
|
metEo-3079::Tn10 |
Linked marker |
est. P1 cotransduction: 14% [9] | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Holmgren, A (1968) Thioredoxin. 6. The amino acid sequence of the protein from escherichia coli B. Eur. J. Biochem. 6 475-84 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Mark, DF et al. (1977) Genetic mapping of trxA, a gene affecting thioredoxin in Escherichia coli K12. Mol. Gen. Genet. 155 145-52 PubMed
- ↑ 5.0 5.1 Russel, M & Model, P (1985) Thioredoxin is required for filamentous phage assembly. Proc. Natl. Acad. Sci. U.S.A. 82 29-33 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).