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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:55, 24 June 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

proC

Mnemonic

Proline

Synonyms

ECK0381, b0386, JW0377, pro2, pro3, pro(3)[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

8.71 minutes 

MG1655: 404868..404059
<gbrowseImage> name=NC_000913:404059..404868 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 373290..372481
<gbrowseImage> name=NC_012967:372481..373290 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 306818..307627
<gbrowseImage> name=NC_012759:306818..307627 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 404868..404059
<gbrowseImage> name=AC_000091:404059..404868 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 344199..343390
<gbrowseImage> name=NC_010473:343390..344199 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

404059

Edman degradation

PMID:6089111[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔproC (Keio:JW0377)

deletion

deletion

Auxotrophies

Requires proline for growth

PMID:16738554[3]

Shigen

CGSC8554[4]

proC14

Auxotrophies

Requires proline for growth

CGSC:4929

proC32

Auxotrophies

Requires proline for growth

CGSC:5085

proC47

Auxotrophies

Requires proline for growth

CGSC:5514

proC34

Auxotrophies

Requires proline for growth

CGSC:5702

proC24

Auxotrophies

Requires proline for growth

CGSC:5825

proC55

Auxotrophies

Requires proline for growth

CGSC:6485

proC83

Auxotrophies

Requires proline for growth

CGSC:10340

proC43

Auxotrophies

Requires proline for growth

CGSC:11027

proC29

Auxotrophies

Requires proline for growth

CGSC:11502

proC9999

Auxotrophies

Requires proline for growth

CGSC:39363

proC677(tetR)::Tn5-132

Auxotrophies

Requires proline for growth

CGSC:75247

proC471::Tn5

Auxotrophies

Requires proline for growth

CGSC:90706

ΔproC751::kan

deletion

deletion

Auxotrophies

Requires proline for growth

PMID:16738554[3]

CGSC:102470


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0377

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGAAAAGAAAATCGGTTTTAT

Primer 2:CCGGATTTGCTGAGTTTTTCTGA

zai-3053::Tn10

Linked marker

CAG18091 = CGSC7336[4]

est. P1 cotransduction: 72% [6]
Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).

tsx-247::Tn10

Linked marker

CAG12148 = CGSC7337[4]

est. P1 cotransduction: 35% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10769

EchoBASE (EcoliWiki Page)

EchoBASE:EB0762

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0386

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0493

EcoGene (EcoliWiki Page)

EcoGene:EG10769

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PROC

CGSC (EcoliWiki Page)

CGSC:362

ASAP (EcoliWikiPage)

ASAP:ABE-0001338


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Deutch, AH et al. (1984) Analysis of the Escherichia coli proBA locus by DNA and protein sequencing. Nucleic Acids Res. 12 6337-55 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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