uspF:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]

Revision as of 23:53, 10 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uspF

Mnemonic

Universal stress protein

Synonyms

ECK1371, b1376, JW1370, ynaF, yzzL, up03[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

30.89 minutes 

MG1655: 1433643..1433209
gbrowse_img.php?coord=1433209..1433643&genome=ecoli&name=MG1655&trackset=0&width=300&.png

BW2952

NC_012759: 1325268..1325702
gbrowse_img.php?coord=1325268..1325702&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 1437333..1436899
gbrowse_img.php?coord=1436899..1437333&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1524238..1523804
gbrowse_img.php?coord=1523804..1524238&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔuspF (Keio:JW1370)

deletion

deletion

PMID:16738554[2]

Shigen

ΔyzzL::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938[3]



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1370

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAACAGAACGATTCTTGTCCC

Primer 2:CCGCGCACAACCAGCACGGAGCA

recT3061::Tn10

Linked marker

CAG12081 = CGSC7375[5]

est. P1 cotransduction: 43% [6]
Synonyms:zcj-3061::Tn10, zda-3061::Tn10

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[5]

est. P1 cotransduction: 99% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6699

EchoBASE (EcoliWiki Page)

EchoBASE:EB2539

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1376

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1821

EcoGene (EcoliWiki Page)

EcoGene:EG12674

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YNAF

ASAP (EcoliWikiPage)

ASAP:ABE-0004605



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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