mutH:Gene: Difference between revisions

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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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Revision as of 22:53, 10 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mutH

Mnemonic

Mutator

Synonyms

ECK2827, b2831, JW2799, mutR, prv[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.96 minutes 

MG1655: 2967684..2968373
gbrowse_img.php?coord=2967684..2968373&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2864282..2864971
gbrowse_img.php?coord=2864282..2864971&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2854832..2855521
gbrowse_img.php?coord=2854832..2855521&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2968318..2969007
gbrowse_img.php?coord=2968318..2969007&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3061554..3062243
gbrowse_img.php?coord=3061554..3062243&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmutH (Keio:JW2799)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10186[3]

mutH::Tn5KAN

PMID:12519969[4]

ASAP

At position 200 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20953

mutH::Tn5KAN

PMID:12519969[4]

ASAP

At position 200 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20954

mutHQ135K

Q135K

(in strain: ECOR 51, ECOR 52, ECOR 57, ECOR 59, ECOR 62 and ECOR 64)

Strain variation; seeded from UniProt:P06722

mutHG64R

G64R

(in strain: ECOR 50)

Strain variation; seeded from UniProt:P06722

mutHD157N

D157N

In TS28; temperature-sensitive

seeded from UniProt:P06722

mutHQ153R

Q153R

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHR155C

R155C

(in strain: ECOR 35)

Strain variation; seeded from UniProt:P06722

mutHT28L

T28L

In TS7; temperature-sensitive

seeded from UniProt:P06722

mutHW58S

W58S

(in strain: ECOR 67)

Strain variation; seeded from UniProt:P06722

mutHG60C

G60C

(in strain: ECOR 13, ECOR 20, ECOR 26 and ECOR 70)

Strain variation; seeded from UniProt:P06722

mutH34

PMID:1092652[5]

CGSC:12133

mutH471::kan

CGSC:30821

mutH472::mini-Tn10

PMID:1649454[6]

CGSC:38814

ΔmutH756::kan

PMID:16738554[2]

CGSC:101972


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2799

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCTCCCAACCTCGCCCACTGCT

Primer 2:CCtTGGATCAGAAAATGACGGGC

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[3]

est. P1 cotransduction: 55% [8]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[3]

est. P1 cotransduction: % [8]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10624

EchoBASE (EcoliWiki Page)

EchoBASE:EB0619

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2831

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3426

EcoGene (EcoliWiki Page)

EcoGene:EG10624

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MUTH

CGSC (EcoliWiki Page)

CGSC:471

ASAP (EcoliWikiPage)

ASAP:ABE-0009287


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Hoess, RH & Herman, RK (1975) Isolation and characterization of mutator strains of Escherichia coli K-12. J. Bacteriol. 122 474-84 PubMed
  6. ↑ Michaels, ML et al. (1991) MutM, a protein that prevents G.C----T.A transversions, is formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 19 3629-32 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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