moaA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="W46a9b88f315fe"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''moaA''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''moaA''
Molybdenate
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK0770, b0781, JW0764, bisA, chlA, chlA1, narA''<ref name='LIB:Riley_2006'/>, narA''


|-
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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.12117.W46a9b88f315fe&page=12117&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK0770, b0781, JW0764, bisA, chlA, chlA1, narA''<ref name='LIB:Riley_2006'/>
|-
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===Notes===
===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9b88fa9e2a"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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|
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MG1655
MG1655
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17.59 minutes&nbsp;
17.59 minutes&nbsp;
|
|
MG1655: 816267..817256<br />{{GBrowseFigureByCoord|815267|818256|ecoli|MG1655}}
MG1655: 816267..817256<br /><gbrowseImage>
name=NC_000913:816267..817256
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11595 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11595 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:815267..818256 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|815267|818256|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:816267..817256&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|816267|817256|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11595 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11595 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 809670..810659<br /><gbrowseImage>
name=NC_012967:809670..810659
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|809670|810659|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|809670|810659|FASTA Dump}}
|-
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[[BW2952]]
|
 
|
NC_012759: 719235..720224<br /><gbrowseImage>
name=NC_012759:719235..720224
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|719235|720224|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|719235|720224|FASTA Dump}}
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 817466..818455<br />{{GBrowseFigureByCoord|816466|819455|ecoli_W3110|W3110}}
W3110: 817466..818455<br /><gbrowseImage>
name=NC_007779:817466..818455
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:816466..819455 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|816466|819455|GBrowse(W3110)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:817466..818455&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|817466|818455|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 870195..871184<br /><gbrowseImage>
name=NC_010473:870195..871184
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|868217|873162|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|868217|873162|FASTA Dump}}
 
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
816267
|
Edman degradation
|
PMID:8361352<br />
|
 
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====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="P46a9b890928e8"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|-
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''moaA(del)'' (Keio:JW0764)
|
deletion
|
deletion
|
 
|
 
|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=107102 CGSC8821]<ref name='LIB:cgsc'/>
|
|-  
|
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&Delta;moaA (Keio:JW0764)
''moaA(del)''::kan
|
|
deletion
deletion
|
|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|Acetate]]
|
PMID:16095938
|
|
|-
|
''moaA(del)''::kan
|
|
deletion
deletion
|
|


|
[[Biolog]]:respiration
|
unable to respire [[Carbon sources|L-Serine]]
|
PMID:16095938
|
|


|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
|-
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
''moaA18''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:4965|CGSC:4965]]
|
|
   
   
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''moaA15''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:10257|CGSC:10257]]
|
|-
|
''moaA1''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:11070|CGSC:11070]]
|
|-
|
''moaA29''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:12219|CGSC:12219]]
|
|-
|
''moaA31''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:74600|CGSC:74600]]
|
|-
|
''moaA35''
|
 
|
 
|
 
|
 
|
 
|
[[cgscmutation:74714|CGSC:74714]]
|
|-
|
''moaA753(del)''::kan
|
 
|
 
|
 
|
 
|
PMID:16738554
|
[[cgscmutation:101898|CGSC:101898]]
|
|-
|
chlA
|
 
|
 
|
Resistant to
|
Resistant to Chlorate
|
PMID:4558444
|
Strain: PK 27
|
Table 7
|-
|
chlA
|
 
|
 
|
Enzyme Activity
|
Lack of formate nitrate reductase activity
|
PMID:4558444
|
Strain: PK 27
|
Table 7
 
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12117.P4e04bf6ea0015-->
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW0764
JW0764
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 186: Line 473:


Primer 2:CCGCCGCCAATGTACGATAAGTT  
Primer 2:CCGCCGCCAATGTACGATAAGTT  
|-
|-
|
[[:Category:Kohara_Phage:4H7|4H7]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=201 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3D4|3D4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=202 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:24F9|24F9]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=203 Genobase]
|
PMID:3038334
|-  
|
|
[[nadA:Gene|nadA57::Tn10]]
[[nadA:Gene|nadA57::Tn10]]
Line 195: Line 509:
|
|
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 22% <ref name='LIB:Nichols_linked_Tn'/>  
|-
|-  
|
|
zbh-29::Tn10
zbh-29::Tn10
Line 203: Line 517:
CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/>
CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 85% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zbi-29::Tn10


Synonyms:zbi-29::Tn10
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===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11595 EcoCyc:EG11595] 
|
|-
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1552 EchoBASE:EB1552] 
EcoCyc:EG11595
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0781 EcoliGenExpDB:b0781] 
EcoGene:EG11595
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C0862 ECOL199310CYC:C0862] 
RegulonDB:ECK120001543
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG11595 EcoGene:EG11595]
[http://www.ncbi.nlm.nih.gov/gene?term=945392 GeneID:945392]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=MOAA ECOO157CYC:MOAA] 
EchoBASE:EB1552
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/922 CGSC:922] 
ASAP:ABE-0002669
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:moa genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:33, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

moaA

Mnemonic

Molybdenate

Synonyms

ECK0770, b0781, JW0764, bisA, chlA, chlA1, narA[1], narA


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.59 minutes 

MG1655: 816267..817256
<gbrowseImage> name=NC_000913:816267..817256 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 809670..810659
<gbrowseImage> name=NC_012967:809670..810659 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 719235..720224
<gbrowseImage> name=NC_012759:719235..720224 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 817466..818455
<gbrowseImage> name=NC_007779:817466..818455 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 870195..871184
<gbrowseImage> name=NC_010473:870195..871184 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

816267

Edman degradation

PMID:8361352[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

moaA(del) (Keio:JW0764)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8821[4]

moaA(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

moaA(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[5]

moaA18

CGSC:4965

moaA15

CGSC:10257

moaA1

CGSC:11070

moaA29

CGSC:12219

moaA31

CGSC:74600

moaA35

CGSC:74714

moaA753(del)::kan

PMID:16738554[3]

CGSC:101898

chlA

Resistant to

Resistant to Chlorate

PMID:4558444[6]

Strain: PK 27

Table 7

chlA

Enzyme Activity

Lack of formate nitrate reductase activity

PMID:4558444[6]

Strain: PK 27

Table 7


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0764

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGCTTCACAACTGACTGATGC

Primer 2:CCGCCGCCAATGTACGATAAGTT

4H7

Kohara Phage

Genobase

PMID:3038334[8]

3D4

Kohara Phage

Genobase

PMID:3038334[8]

24F9

Kohara Phage

Genobase

PMID:3038334[8]

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 22% [9]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 85% [9]
Synonyms:zbi-29::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11595

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11595

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001543

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945392

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1552

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002669

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Rivers, SL et al. (1993) Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol. Microbiol. 8 1071-81 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. ↑ 6.0 6.1 Glaser, JH & DeMoss, JA (1972) Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol. Gen. Genet. 116 1-10 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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