moaA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

moaA

Mnemonic

Molybdenate

Synonyms

ECK0770, b0781, JW0764, bisA, chlA, chlA1, narA[1], narA

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.59 minutes 

MG1655: 816267..817256
<gbrowseImage> name=NC_000913:816267..817256 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 809670..810659
<gbrowseImage> name=NC_012967:809670..810659 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 719235..720224
<gbrowseImage> name=NC_012759:719235..720224 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 817466..818455
<gbrowseImage> name=NC_007779:817466..818455 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 870195..871184
<gbrowseImage> name=NC_010473:870195..871184 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

816267

Edman degradation

PMID:8361352[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

moaA(del) (Keio:JW0764)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8821[4]

moaA(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[5]

moaA(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938[5]

moaA18

CGSC:4965

moaA15

CGSC:10257

moaA1

CGSC:11070

moaA29

CGSC:12219

moaA31

CGSC:74600

moaA35

CGSC:74714

moaA753(del)::kan

PMID:16738554[3]

CGSC:101898

chlA

Resistant to

Resistant to Chlorate

PMID:4558444[6]

Strain: PK 27

Table 7

chlA

Enzyme Activity

Lack of formate nitrate reductase activity

PMID:4558444[6]

Strain: PK 27

Table 7

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0764

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGCTTCACAACTGACTGATGC

Primer 2:CCGCCGCCAATGTACGATAAGTT

4H7

Kohara Phage

Genobase

PMID:3038334[8]

3D4

Kohara Phage

Genobase

PMID:3038334[8]

24F9

Kohara Phage

Genobase

PMID:3038334[8]

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 22% [9]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 85% [9]
Synonyms:zbi-29::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11595

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11595

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001543

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945392

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1552

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002669

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Rivers, SL et al. (1993) Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol. Microbiol. 8 1071-81 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  6. 6.0 6.1 Glaser, JH & DeMoss, JA (1972) Comparison of nitrate reductase mutants of Escherichia coli selected by alternative procedures. Mol. Gen. Genet. 116 1-10 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 8.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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