ompF:Gene: Difference between revisions

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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{| id="G46a9bbe11b43e"  class=" tableEdit Gene_nomenclature_table"   
{|   id="G46a9bbe11b43e"  class=" tableEdit Gene_nomenclature_table"   


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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK0920, b0929, JW0912, cmlB, coa, cry, tolF, nfxB?''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
''ECK0920, b0929, JW0912, cmlB, coa, cry, tolF, nfxB?''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>, nfxB?''


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*  
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****************************************************************************************** -->{{Gene_location_help}}
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{|   id="K46a9bbe19454c"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9bbe19454c"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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21.23 minutes&nbsp;
21.23 minutes&nbsp;
|
|
MG1655: 986205..985117<br />{{GBrowseFigureByCoord|985117|986205|ecoli|MG1655}}
MG1655: 986205..985117<br /><gbrowseImage>
name=NC_000913:985117..986205
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10671 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10671 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|984117|987205|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|984117|987205|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|985117|986205|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|985117|986205|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10671 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10671 EcoCyc Sequence Dump]  
|-  
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|
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NC_012967: 1004074..1002986<br />{{GBrowseFigureByCoord|1002986|1004074|REL606|NC_012967}}
NC_012967: 1004074..1002986<br /><gbrowseImage>
name=NC_012967:1002986..1004074
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
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{{GBrowseLink|REL606|NC_012967|1002986|1004074|GBrowse (REL606)}}
{{GBrowseLink|REL606|NC_012967|1002986|1004074|GBrowse (REL606)}}
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|
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NC_012759: 888085..889173<br />{{GBrowseFigureByCoord|888085|889173|BW2952|NC_012759}}
NC_012759: 888085..889173<br /><gbrowseImage>
name=NC_012759:888085..889173
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
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{{GBrowseLink|BW2952|012759|888085|NC_889173|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|888085|889173|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|012759|888085|NC_889173|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|888085|889173|FASTA Dump}}  
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&nbsp;
&nbsp;
|
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W3110: 987404..986316<br />{{GBrowseFigureByCoord|986316|987404|ecoli_W3110|W3110}}
W3110: 987404..986316<br /><gbrowseImage>
name=NC_007779:986316..987404
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|985316|988404|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|985316|988404|GBrowse(W3110)}}  
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|986316|987404|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|986316|987404|FASTA Dump}}  
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DH10B: 1040133..1039045<br />{{GBrowseFigureByCoord|1039045|1040133|ecoli_DH10B|DH10B}}
DH10B: 1040133..1039045<br /><gbrowseImage>
name=NC_010473:1039045..1040133
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
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{{GBrowseLink|ecoli_DH10B|NC_010473|1036869|1042309|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|1036869|1042309|GBrowse(DH10B)}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|1036869|1042309|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|1036869|1042309|FASTA Dump}}  


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{|   id="B46a9bbe23ea33"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46a9bbe23ea33"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| {{Prettytable}} id="W46a9bbe27f29e"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="W46a9bbe27f29e"  class=" tableEdit Gene_allele_table dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
|-  
|
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&Delta;ompF (Keio:JW0912)
''ompF(del)'' (Keio:JW0912)
|
|
deletion
deletion
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PMID:16738554
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107206 CGSC8925]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=107206 CGSC8925]<ref name='LIB:cgsc'/>  
|
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''&#916;ompF746::kan''
''ompF746(del)''::kan
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[[cgscmutation:102178|CGSC:102178]]
[[cgscmutation:102178|CGSC:102178]]
|
|-
|
ompFp9
|
|
|
protein expression
|
OmpF is constitutively expressed in ompFp9 mutants, there is less repression than in wild-type strain
|
PMID:7007334
|
|
|-
|
''ompF<sup>  -</sup>''
|
|
|
Resistant to
|
Resistance to Cloacin DF13
|
PMID:2013565
|
Strain: LG1702
|
Figure 3
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Polymyxin
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin A
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin E2
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin E3
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin E3
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin K
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin K
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin L
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Colicin L-JF246
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Chloramphenicol
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to tetracycline
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF'' in strain JF557
|
|
|
Resistant to
|
Resistance to Oxytetracycline
|
PMID:789358
|
Strain: JF557
|
TABLE 5
|-
|
''ompF1466''
|
|
|
Resistant to
|
Resistance to beta lactam ampicillin
|
PMID:6760806
|
Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11)
|
Mutant is characterized by lack of ompF in strain B1466. Please see table 3.
|-
|
''ompF1466''
|
|
|
Resistant to
|
Resistance to beta lactam cefoxitin
|
PMID:6760806
|
Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11)
|
Mutant is characterized by lack of ''ompF'' in strain B1466. Please see table 3.
|-
|
''tolF'' in mutant strain JF557
|
|
|
Resistant to
|
Resistant to Tetracycline
|
PMID:789358
|
Strain: JF557
|
Table 5
|-
|
''tolF'' in mutant strain JF557
|
|
|
Resistant to
|
Resistant to Chloramphenicol
|
PMID:789358
|
Strain: JF557
|
Table 5
|-
|
''ompF'' in strain JF703
|
|
|
Resistant to
|
Resistance to Colicin A
|
PMID:7047502
|
Strain: JF703
|
table 2
|-
|
''ompF<sup>-</sup>''
|
|
|
Resistant to
|
Introduction of ompF mutation caused a partial resistance to Microcin B17.
|
PMID:3543211
|
|
See table 4 for complete experimental results. 
|-
|
''ompF<sup>-</sup>'' ''recA<sup>-</sup>''
|
|
|
Resistant to
|
Introduction of double mutation caused an increase in resistance to Microcin B17.
|
PMID:3543211
|
|
See table 4 for complete experimental results. 
|-
|
''cmlB5''
|
|
|
Resistant to
|
Resistant to choramphenicol as measured by zones of growth inhibition.
|
PMID:374348
|
Parental Strain: RE1 [http://cgsc.biology.yale.edu/Strain.php?ID=14205 CGSC]
Experimental Strain: RE107 [http://cgsc.biology.yale.edu/Strain.php?ID=11643 CGSC]
|
Growth Inhibition experiments Figure 2
|-
|
''ompF'' in mutant strain CE1121
|
|
|
Resistant to
|
Resistant to TuIa phage.
|
PMID:375003
|
Experimental Strain: CE1121
Parental Strain: PC0479
|
|-
|
''ompF627'' ''fadL701''
|
|
|
Resistant to
|
Resistance to phage T2
|
PMID:3286621
|
Experimental Strain: E15
|
|
   
   
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===
Reduced levels of OmpF gives multiple antibiotic resistance. <ref name='PMID:11601842'/>
==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12417.Q4e04c07d3b9f7-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q4e04c07d3b9f7"  class=" tableEdit Genetic_interaction_table dataTable" 
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes


|- class="tableEdit_footer"
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===Notes===


==Genetic Resources==
==Genetic Resources==
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{|   id="F46a9bbe3299f0"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="F46a9bbe3299f0"  class=" tableEdit Gene_resource_table"   
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!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCGAACTGGTAAACGATACCCAC  
Primer 2:CCGAACTGGTAAACGATACCCAC  
|-
|
[[:Category:Kohara_Phage:E1D1|E1D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=218 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:11F5|11F5]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=219 Genobase]
|
PMID:3038334
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{|   id="A46a9bbe3b3bab"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="A46a9bbe3b3bab"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10671 EcoCyc:EG10671]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0665 EchoBASE:EB0665]
|
|-
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0929 EcoliGenExpDB:b0929]
EcoCyc:EG10671
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C1071 ECOL199310CYC:C1071]
EcoGene:EG10671
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10671 EcoGene:EG10671]
RegulonDB:ECK120000664
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=OMPF ECOO157CYC:OMPF]
[http://www.ncbi.nlm.nih.gov/gene?term=945554 GeneID:945554]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/435 CGSC:435]
EchoBASE:EB0665
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003153&formSubmitReturn=1 ASAP:ABE-0003153]
ASAP:ABE-0003153
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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!|Name!!URL!!Comments
!|Name!!URL!!Comments

Latest revision as of 20:32, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ompF

Mnemonic

Outer membrane protein

Synonyms

ECK0920, b0929, JW0912, cmlB, coa, cry, tolF, nfxB?[1][2], nfxB?

edit table


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.23 minutes 

MG1655: 986205..985117
<gbrowseImage> name=NC_000913:985117..986205 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1004074..1002986
<gbrowseImage> name=NC_012967:1002986..1004074 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 888085..889173
<gbrowseImage> name=NC_012759:888085..889173 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 987404..986316
<gbrowseImage> name=NC_007779:986316..987404 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1040133..1039045
<gbrowseImage> name=NC_010473:1039045..1040133 source=DH10B preset=GeneLocation </gbrowseImage>

edit table


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

985120

Edman degradation

PMID:7001461[3]
PMID:9298646[4]
PMID:9629924[5]


edit table


Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ompF(del) (Keio:JW0912)

deletion

deletion

PMID:16738554[6]

Shigen

CGSC8925[7]

ompF627(T2R)

T2 resistant

PMID:3286621[8]

CGSC:4722

ompF626

CGSC:6264

ompF254

CGSC:6819

ompF625

CGSC:11640

ompF746(del)::kan

PMID:16738554[6]

CGSC:102178

ompFp9

protein expression

OmpF is constitutively expressed in ompFp9 mutants, there is less repression than in wild-type strain

PMID:7007334[9]

ompF -

Resistant to

Resistance to Cloacin DF13

PMID:2013565[10]

Strain: LG1702

Figure 3

ompF in strain JF557

Resistant to

Resistance to Polymyxin

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin A

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin E2

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin E3

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin E3

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin K

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin K

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin L

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Colicin L-JF246

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Chloramphenicol

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to tetracycline

PMID:789358[11]

Strain: JF557

TABLE 5

ompF in strain JF557

Resistant to

Resistance to Oxytetracycline

PMID:789358[11]

Strain: JF557

TABLE 5

ompF1466

Resistant to

Resistance to beta lactam ampicillin

PMID:6760806[12]

Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11)

Mutant is characterized by lack of ompF in strain B1466. Please see table 3.

ompF1466

Resistant to

Resistance to beta lactam cefoxitin

PMID:6760806[12]

Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11)

Mutant is characterized by lack of ompF in strain B1466. Please see table 3.

tolF in mutant strain JF557

Resistant to

Resistant to Tetracycline

PMID:789358[11]

Strain: JF557

Table 5

tolF in mutant strain JF557

Resistant to

Resistant to Chloramphenicol

PMID:789358[11]

Strain: JF557

Table 5

ompF in strain JF703

Resistant to

Resistance to Colicin A

PMID:7047502[13]

Strain: JF703

table 2

ompF-

Resistant to

Introduction of ompF mutation caused a partial resistance to Microcin B17.

PMID:3543211[14]

See table 4 for complete experimental results.

ompF- recA-

Resistant to

Introduction of double mutation caused an increase in resistance to Microcin B17.

PMID:3543211[14]

See table 4 for complete experimental results.

cmlB5

Resistant to

Resistant to choramphenicol as measured by zones of growth inhibition.

PMID:374348[15]

Parental Strain: RE1 CGSC


Experimental Strain: RE107 CGSC

Growth Inhibition experiments Figure 2

ompF in mutant strain CE1121

Resistant to

Resistant to TuIa phage.

PMID:375003[16]

Experimental Strain: CE1121

Parental Strain: PC0479

ompF627 fadL701

Resistant to

Resistance to phage T2

PMID:3286621[8]

Experimental Strain: E15



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Notes

Reduced levels of OmpF gives multiple antibiotic resistance. [17]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0912

Plasmid clone

Shigen

PMID:16769691[18]

Status:Clone OK

Primer 1:GCCATGAAGCGCAATATTCTGGC

Primer 2:CCGAACTGGTAAACGATACCCAC

E1D1

Kohara Phage

Genobase

PMID:3038334[19]

11F5

Kohara Phage

Genobase

PMID:3038334[19]

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[7]

est. P1 cotransduction: 20% [20]
Synonyms:zbj-1230::Tn10

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[7]

est. P1 cotransduction: 54% [20]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10671

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10671

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000664

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945554

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0665

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003153

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Chen, R et al. (1980) Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12. Proc. Natl. Acad. Sci. U.S.A. 77 4592-6 PubMed
  4. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  5. ↑ Molloy, MP et al. (1998) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 837-44 PubMed
  6. ↑ 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 Black, PN (1988) The fadL gene product of Escherichia coli is an outer membrane protein required for uptake of long-chain fatty acids and involved in sensitivity to bacteriophage T2. J. Bacteriol. 170 2850-4 PubMed
  9. ↑ Sato, T & Yura, T (1981) Regulatory mutations conferring constitutive synthesis of major outer membrane proteins (OmpC and OmpF) in Escherichia coli. J. Bacteriol. 145 88-96 PubMed
  10. ↑ Wooldridge, KG & Williams, PH (1991) Sensitivity of Escherichia coli to cloacin DF13 involves the major outer membrane protein OmpF. J. Bacteriol. 173 2420-4 PubMed
  11. ↑ 11.00 11.01 11.02 11.03 11.04 11.05 11.06 11.07 11.08 11.09 11.10 11.11 11.12 11.13 Foulds, J (1976) TolF locus in Escherichia coli: chromosomal location and relationship to loci cmlB and tolD. J. Bacteriol. 128 604-8 PubMed
  12. ↑ 12.0 12.1 Jaffe, A et al. (1982) Role of porin proteins OmpF and OmpC in the permeation of beta-lactams. Antimicrob. Agents Chemother. 22 942-8 PubMed
  13. ↑ Chai, T et al. (1982) Colicin A receptor: role of two Escherichia coli outer membrane proteins (OmpF protein and btuB gene product) and lipopolysaccharide. J. Bacteriol. 151 983-8 PubMed
  14. ↑ 14.0 14.1 Laviña, M et al. (1986) Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. J. Gen. Microbiol. 132 1685-93 PubMed
  15. ↑ Baughman, GA & Fahnestock, SR (1979) Chloramphenicol resistance mutation in Escherichia coli which maps in the major ribosomal protein gene cluster. J. Bacteriol. 137 1315-23 PubMed
  16. ↑ Verhoef, C et al. (1979) Genetics and biochemistry of the peptidoglycan-associated proteins b and c of Escherichia coli K12. Mol. Gen. Genet. 169 137-46 PubMed
  17. ↑ Delihas, N & Forst, S (2001) MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J. Mol. Biol. 313 1-12 PubMed
  18. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  19. ↑ 19.0 19.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  20. ↑ 20.0 20.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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