lexA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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*  
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{| id="F46aa058a7e25a"  class=" tableEdit Gene_nomenclature_table"   
{|   id="F46aa058a7e25a"  class=" tableEdit Gene_nomenclature_table"   


|-
|-
!align=left {{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
 
''lexA''
|-
|-
!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
Lambda excision
|-
|-
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
 
''ECK4035, b4043, JW4003, exrA, tsl, umuA''<ref name='LIB:Riley_2006'/>, spr, umuA''


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*  
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{|   id="Q46aa058b0e011"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q46aa058b0e011"  class=" tableEdit Gene_location_table"   
|-
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-  
|-  
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91.71 minutes&nbsp;
91.71 minutes&nbsp;
|
|
MG1655: 4255138..4255746<br />{{GBrowseFigureByCoord|4255138|4255746|ecoli|MG1655}}
MG1655: 4255138..4255746<br /><gbrowseImage>
name=NC_000913:4255138..4255746
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10533 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10533 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|4254138|4256746|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|4254138|4256746|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|4255138|4255746|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|4255138|4255746|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10533 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10533 EcoCyc Sequence Dump]  
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NC_012967: 4236047..4236655<br />{{GBrowseFigureByCoord|4236047|4236655|REL606|NC_012967}}
NC_012967: 4236047..4236655<br /><gbrowseImage>
name=NC_012967:4236047..4236655
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|REL606|NC_012967|4236047|4236655|GBrowse (REL606)}}
{{GBrowseLink|REL606|NC_012967|4236047|4236655|GBrowse (REL606)}}
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{{GBrowseFastaLink|REL606|NC_012967|4236047|4236655|FASTA Dump}}  
{{GBrowseFastaLink|REL606|NC_012967|4236047|4236655|FASTA Dump}}  
|-
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[[BW2952]]
|
|
NC_012759: 4193983..4194591<br /><gbrowseImage>
name=NC_012759:4193983..4194591
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|NC_012759|4193983|4194591|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|NC_012759|4193983|4194591|FASTA Dump}}
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&nbsp;
&nbsp;
|
|
W3110: 4260705..4261313<br />{{GBrowseFigureByCoord|4260705|4261313|ecoli_W3110|W3110}}
W3110: 4260705..4261313<br /><gbrowseImage>
name=NC_007779:4260705..4261313
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|4259705|4262313|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|4259705|4262313|GBrowse(W3110)}}  
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|4260705|4261313|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|4260705|4261313|FASTA Dump}}  
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|
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DH10B: 4354834..4355442<br />{{GBrowseFigureByCoord|4354834|4355442|ecoli_DH10B|DH10B}}
DH10B: 4354834..4355442<br /><gbrowseImage>
name=NC_010473:4354834..4355442
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_DH10B|NC_010473|4353618|4356658|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|4353618|4356658|GBrowse(DH10B)}}
|
|
{{GBrowseFastaLink|ecoli_DH10B|NC_010473|4353618|4356658|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|4353618|4356658|FASTA Dump}}  


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{|   id="L46aa058bc99aa"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="L46aa058bc99aa"  class=" tableEdit Gene_sequence_features_table"   
|-
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18645.L46aa058bc99aa&page=18645&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table]</span> || || || || ||
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<protect><!--Sequence_notes--></protect>
<protect><!--Sequence_notes--></protect>
====Notes====
====Notes====
 
A description of the ''lexA'' gene may be found in Markham ''et al.'' (1981)<ref name='PMID:6272195'/>.


==Alleles and Phenotypes==
==Alleles and Phenotypes==
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*  
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****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{| {{Prettytable}} id="D46aa058c206f8"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="D46aa058c206f8"  class=" tableEdit Gene_allele_table dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
|-  
|
|
spr
lexAG85D
|
|


|
G85D
|
|


|
|
(in lexA3, resistant to cleavage)
|
|


|
PMID:2834329
PMID: 7038393
|
|


|
|
uninducible, not cleaved during SOS response
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0A7C2 UniProt:P0A7C2]
|-  
|-  
|
|
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|-  
|-  
|
|
lexAG85D
''lexA2''
|
C461T
|
T154I
|
Sensitivity to
|
hypocleavable repressor leads to a sensitivity to DNA damage
|
PMID:3127383
|
 
|
|-
|
spr
|
 
|
 
|
 
|
 
|
PMID:2834329
PMID:7038393
|
 
|
uninducible, not cleaved during SOS response
|-
|
''lexA264'' (Silent SalI site )
|
GGT->GTT
|
G80V
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as the mutagen
|-
|
''lexA261'' (Silent SalI site )
|
GGT->GAT
|
G80D
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Hydroxylamine was used as the mutagen
|-
|
''lexA266'' (Silent SalI site )
|
GTG->GAG
|
V82E
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as the mutagen
|-
|
''lexA265'' (Silent SalI site )
|
GTG->ATG
|
V82M
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Both formic acid and hydroxylamine were used as to mutagenize the strain.
|-
|
''lexA262'' (Silent SalI site )
|
GCT->GTT
|
A83V
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Hydroxylamine were used as to mutagenize the strain.
|-
|
''lexA267'' (Silent ''SalI'' site )
|
GCT->ACT
|
A83T
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Both hydroxylamine and hydrazine were used as to mutagenize the strain.
|-
|
''lexA252'' (Silent ''SalI'' site )
|
GCT->--
|
A83&Delta;
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Spontaneous mutation
|-
|
''lexA260'' (Silent ''SalI'' site )
|
GCC->ACC
|
A84T
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Hydroxylamine was used as to mutagenize the strain.
|-
|
''lexA268'' (Silent ''SalI'' site )
|
GCC->GAC
|
A84D
|
 
|
Ind- phenotype
|
PMID:2834329
|
|


|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA3'' (Silent ''SalI'' site )
|
GGT->GAT
|
|
G85D
G85D
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|
|
(in lexA3, resistant to cleavage)
Ind- phenotype
|
PMID:2834329
|
 
|
Hydrazine was used as to mutagenize the strain.
|-
|
''lexA252'' (Silent ''SalI'' site )
|
GAA->AAA
|
A95K
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Spontaneous mutation
|-
|
''lexA274'' (Silent ''SalI'' site )
|
GGC->CCC
|
R114P
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA251'' (Silent SalI site )
|
TCG->TTG
|
S119L
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
This allele was produced spontaneously and using hydroxylamine.
|-
|
''lexA269'' (Silent ''SalI'' site )
|
GTC->TTC
|
V115F
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA280'' (Silent ''SalI'' site )
|
ATG->TTG
|
M120L
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA263'' (Silent SalI site )
|
GGG->GAG
|
G117E
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Both formic acid and hydroxylamine were used as to mutagenize the strain.
|-
|
''lexA259'' (Silent ''SalI'' site )
|
ACC->ATC
|
T154I
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Both hydrazine and hydroxylamine were used as to mutagenize the strain.
|-
|
''lexA270'' (Silent ''SalI'' site )
|
AAG->AGG
|
K156R
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA272'' (Silent ''SalI'' site )
|
AAG->ATG
|
K156M
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA271-281''
|
 
|
K156N/Q184H
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA271'' (Silent ''SalI'' and ''BglI'' site)
|
AAG->AAT
|
K156N
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
|-
|
''lexA281'' (Silent ''SalI'' and ''BstI'' site)
|
CAG->CAT
|
Q184H
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
|-
|
''lexA273-282''
|
 
|
K156T/E184V
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
Formic acid was used as to mutagenize the strain.
|-
|
''lexA273'' (Silent ''SalI and BglI'' sites)
|
AAG->ACG
|
K156T
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
|-
|
''lexA282'' (Silent ''SalI and BglI'' sites)
|
GAA->GTA
|
E170V
|
 
|
Ind- phenotype
|
PMID:2834329
|
 
|
|-
|
lexA3 in [[MDS42]]
|
deletion
|
|


|
Sensitivity to
|
increases senstitivity to [[Bicyclomycin]]
|
PMID:21183718
|
|


|
|
Strain variation; seeded from [http://www.uniprot.org/uniprot/P0A7C2 UniProt:P0A7C2]
fig. 2
|-
|
SMR6039 ''lexA3(Ind<sup>-</sup>)MalB''::Tn9
|
insertion
|
 
|
SOS response
|
Decrease in spontaneous SOS-induction
|
PMID:23224554
|
Parent Strain: SMR6039
Experimental Strain: SMR6178
|
The mutation caused a defect in spontaneous SOS-induction. See table S7 for a summary of experimental results.


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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.18645.E4e04be5d4dcbe-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="E4e04be5d4dcbe"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18645.E4e04be5d4dcbe&page=18645&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
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===Notes===


==Genetic Resources==
==Genetic Resources==
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{|   id="K46aa058cdba1d"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aa058cdba1d"  class=" tableEdit Gene_resource_table"   
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|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Line 469: Line 1,007:


Primer 2:CCaAGCCAGTCGCCGTTGCGAAT  
Primer 2:CCaAGCCAGTCGCCGTTGCGAAT  
|-
|
[[:Category:Kohara_Phage:1F8|1F8]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=634 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:12B4|12B4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=635 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:1E4|1E4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=636 Genobase]
|
PMID:3038334
|-  
|-  
|
|
Line 487: Line 1,052:
|
|
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 9% <ref name='LIB:Nichols_linked_Tn'/>  
|-
|
DM Strain List
|
Strain
|
There are several strains with single ''lexA'' mutations listed in Table 1.
|
PMID:4343824


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|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10533 EcoCyc:EG10533]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0528 EchoBASE:EB0528]
EcoCyc:EG10533
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b4043 EcoliGenExpDB:b4043]
EcoGene:EG10533
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C5014 ECOL199310CYC:C5014]
RegulonDB:ECK120000526
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10533 EcoGene:EG10533]
[http://www.ncbi.nlm.nih.gov/gene?term=948544 GeneID:948544]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=LEXA ECOO157CYC:LEXA]
EchoBASE:EB0528
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/558 CGSC:558]
ASAP:ABE-0013241
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0013241&formSubmitReturn=1 ASAP:ABE-0013241]
|


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[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:lex genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:lex genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:51, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lexA

Mnemonic

Lambda excision

Synonyms

ECK4035, b4043, JW4003, exrA, tsl, umuA[1], spr, umuA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.71 minutes 

MG1655: 4255138..4255746
<gbrowseImage> name=NC_000913:4255138..4255746 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4236047..4236655
<gbrowseImage> name=NC_012967:4236047..4236655 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4193983..4194591
<gbrowseImage> name=NC_012759:4193983..4194591 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4260705..4261313
<gbrowseImage> name=NC_007779:4260705..4261313 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4354834..4355442
<gbrowseImage> name=NC_010473:4354834..4355442 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

A description of the lexA gene may be found in Markham et al. (1981)[2].

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lexAG85D

G85D

(in lexA3, resistant to cleavage)

Strain variation; seeded from UniProt:P0A7C2

lexA107

CGSC:6267

lexA1(Ind-)

non-inducible SOS response

CGSC:9237

lexA41(ts)

temperature sensitive

CGSC:9601

lexA3(Ind-)

non-inducible SOS response

CGSC:9602

lexA51

CGSC:10280

lexA101

CGSC:11964

lexA102

CGSC:11979

lexA109

CGSC:11985

lexA103

CGSC:14425

lexA104

CGSC:14427

lexA105

CGSC:14429

lexA106

CGSC:14431

lexA108

CGSC:14439

lexA71::Tn5

CGSC:30813

lexA2

C461T

T154I

Sensitivity to

hypocleavable repressor leads to a sensitivity to DNA damage

PMID:3127383[3]

spr

PMID:2834329[4] PMID:7038393[5]

uninducible, not cleaved during SOS response

lexA264 (Silent SalI site )

GGT->GTT

G80V

Ind- phenotype

PMID:2834329[4]

Formic acid was used as the mutagen

lexA261 (Silent SalI site )

GGT->GAT

G80D

Ind- phenotype

PMID:2834329[4]

Hydroxylamine was used as the mutagen

lexA266 (Silent SalI site )

GTG->GAG

V82E

Ind- phenotype

PMID:2834329[4]

Formic acid was used as the mutagen

lexA265 (Silent SalI site )

GTG->ATG

V82M

Ind- phenotype

PMID:2834329[4]

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA262 (Silent SalI site )

GCT->GTT

A83V

Ind- phenotype

PMID:2834329[4]

Hydroxylamine were used as to mutagenize the strain.

lexA267 (Silent SalI site )

GCT->ACT

A83T

Ind- phenotype

PMID:2834329[4]

Both hydroxylamine and hydrazine were used as to mutagenize the strain.

lexA252 (Silent SalI site )

GCT->--

A83Δ

Ind- phenotype

PMID:2834329[4]

Spontaneous mutation

lexA260 (Silent SalI site )

GCC->ACC

A84T

Ind- phenotype

PMID:2834329[4]

Hydroxylamine was used as to mutagenize the strain.

lexA268 (Silent SalI site )

GCC->GAC

A84D

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA3 (Silent SalI site )

GGT->GAT

G85D

Ind- phenotype

PMID:2834329[4]

Hydrazine was used as to mutagenize the strain.

lexA252 (Silent SalI site )

GAA->AAA

A95K

Ind- phenotype

PMID:2834329[4]

Spontaneous mutation

lexA274 (Silent SalI site )

GGC->CCC

R114P

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA251 (Silent SalI site )

TCG->TTG

S119L

Ind- phenotype

PMID:2834329[4]

This allele was produced spontaneously and using hydroxylamine.

lexA269 (Silent SalI site )

GTC->TTC

V115F

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA280 (Silent SalI site )

ATG->TTG

M120L

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA263 (Silent SalI site )

GGG->GAG

G117E

Ind- phenotype

PMID:2834329[4]

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA259 (Silent SalI site )

ACC->ATC

T154I

Ind- phenotype

PMID:2834329[4]

Both hydrazine and hydroxylamine were used as to mutagenize the strain.

lexA270 (Silent SalI site )

AAG->AGG

K156R

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA272 (Silent SalI site )

AAG->ATG

K156M

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA271-281

K156N/Q184H

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA271 (Silent SalI and BglI site)

AAG->AAT

K156N

Ind- phenotype

PMID:2834329[4]

lexA281 (Silent SalI and BstI site)

CAG->CAT

Q184H

Ind- phenotype

PMID:2834329[4]

lexA273-282

K156T/E184V

Ind- phenotype

PMID:2834329[4]

Formic acid was used as to mutagenize the strain.

lexA273 (Silent SalI and BglI sites)

AAG->ACG

K156T

Ind- phenotype

PMID:2834329[4]

lexA282 (Silent SalI and BglI sites)

GAA->GTA

E170V

Ind- phenotype

PMID:2834329[4]

lexA3 in MDS42

deletion

Sensitivity to

increases senstitivity to Bicyclomycin

PMID:21183718[6]

fig. 2

SMR6039 lexA3(Ind-)MalB::Tn9

insertion

SOS response

Decrease in spontaneous SOS-induction

PMID:23224554[7]

Parent Strain: SMR6039 Experimental Strain: SMR6178

The mutation caused a defect in spontaneous SOS-induction. See table S7 for a summary of experimental results.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4003

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCAAAGCGTTAACGGCCAGGCA

Primer 2:CCaAGCCAGTCGCCGTTGCGAAT

1F8

Kohara Phage

Genobase

PMID:3038334[9]

12B4

Kohara Phage

Genobase

PMID:3038334[9]

1E4

Kohara Phage

Genobase

PMID:3038334[9]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[10]

est. P1 cotransduction: 60% [11]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[10]

est. P1 cotransduction: 9% [11]

DM Strain List

Strain

There are several strains with single lexA mutations listed in Table 1.

PMID:4343824[12]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10533

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10533

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000526

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948544

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0528

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013241

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Markham, BE et al. (1981) Nucleotide sequence of the lexA gene of Escherichia coli K-12. Nucleic Acids Res. 9 4149-61 PubMed
  3. ↑ Peterson, KR et al. (1988) The Escherichia coli K-12 lexA2 gene encodes a hypocleavable repressor. J. Bacteriol. 170 1975-7 PubMed
  4. ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 4.18 4.19 4.20 4.21 4.22 4.23 4.24 4.25 Lin, LL & Little, JW (1988) Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12. J. Bacteriol. 170 2163-73 PubMed
  5. ↑ Froehlich, B (1981) Weigle reactivation of the single-stranded DNA phage f1. Mol. Gen. Genet. 184 416-20 PubMed
  6. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
  7. ↑ Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 9.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. ↑ 10.0 10.1 CGSC: The Coli Genetics Stock Center
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  12. ↑ Mount, DW et al. (1972) Dominant mutations (lex) in Escherichia coli K-12 which affect radiation sensitivity and frequency of ultraviolet lght-induced mutations. J. Bacteriol. 112 886-93 PubMed

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