dnaK:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
|}
[[Category:Gene List:MG1655]][[Category:Gene List:W3110]][[Category:Genes in the Keio knockout collection]][[Category:Genes in the ASKA clone collection]]
</noinclude><protect>
</noinclude><protect>
==Nomenclature==
==Nomenclature==
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|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
|
||
''dnaK''  
''dnaK''
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
|
||
 
DNA
|-
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
''ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
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===Notes===
===Notes===
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="A46a9a67157a4a"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
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MG1655
MG1655
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0.26 minutes&nbsp;
0.26 minutes&nbsp;
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MG1655: 12163..14079<br />{{GBrowseFigureByCoord|11163|15079|ecoli|MG1655}}
MG1655: 12163..14079<br /><gbrowseImage>
name=NC_000913:12163..14079
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10241 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10241 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:11163..15079 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|11163|15079|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:12163..14079&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|12163|14079|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10241 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10241 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 12161..14077<br /><gbrowseImage>
name=NC_012967:12161..14077
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|12161|14077|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|12161|14077|FASTA Dump}}
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[[BW2952]]
|
 
|
NC_012759: 12163..14079<br /><gbrowseImage>
name=NC_012759:12163..14079
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|12163|14079|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|12163|14079|FASTA Dump}}
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W3110
W3110
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&nbsp;
&nbsp;
|
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W3110: 12163..14079<br />{{GBrowseFigureByCoord|11163|15079|ecoli_W3110|W3110}}
W3110: 12163..14079<br /><gbrowseImage>
name=NC_007779:12163..14079
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:11163..15079 GBrowse(W3110)]
{{GBrowseLink|W3110|NC_007779|11163|15079|GBrowse(W3110)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:12163..14079&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|W3110|NC_007779|12163|14079|FASTA Dump}}
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[[DH10B]]
|
 
|
DH10B: 12163..14079<br /><gbrowseImage>
name=NC_010473:12163..14079
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|8331|17911|GBrowse(DH10B)}}
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{{GBrowseFastaLink|DH10B|NC_010473|8331|17911|FASTA Dump}}
 
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-class='sortbottom'
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Coding Start (SO:0000323)
|
[[MG1655]]
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12166
|
Edman degradation
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PMID:1396676<br />PMID:7783627<br />PMID:9298646<br />
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9a6723f287"  class=" tableEdit Gene_allele_table dataTable" 
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|-
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''dnaK(del)'' (Keio:JW0013)
|
deletion
|
deletion
|
 
|
 
|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=106623 CGSC8342]<ref name='LIB:cgsc'/>
|
|-
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dnaKG32D
|
 
|
G32D
|
 
|
In SEG-1 and dnaK756(TS); confers temperature sensitivity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]
|-
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dnaKV436I
|
 
|
V436I
|
 
|
In mutant SEG-2; confers temperature sensitivity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]
|-
|
dnaKG455D
|
 
|
G455D
|
 
|
In mutant dnaK756(TS); confers temperature sensitivity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]
|-
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dnaKG468D
|
 
|
G468D
|
 
|
In mutant dnaK756(TS); confers temperature sensitivity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]
|-
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''dnaK756''(ts)
|
 
|
 
|
 
|
temperature sensitive
|
 
|
[[cgscmutation:6707|CGSC:6707]]
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&Delta;dnaK (Keio:JW0013)
''dnaK(del)734::kan''
|
|
deletion
deletion
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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
[[cgscmutation:100628|CGSC:100628]]
|
|
   
   
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dnaK
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deletion
|
 
|
Sensitivity to
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increases sensitivity to bicyclomycin
|
PMID:21357484
|
 
|
fig 2
|-
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DnaKG384A
|
 
|
Glycine to Alanine at position 384.
|
 
|
There is a 13 fold decrease rate for substrate release in ATP bound state and increase in substrate affinity. with an overall 2.5 fold higher chaperon activity, Table 1.
|
PMID:21656889
|
 
|
|-
|
G384A/Cd35
|
 
|
Double mutant
|
 
|
The double mutant combines accelerated peptide binding in its ADP-bound state (like Cd35) and slower peptide release in its ATP-bound state (likeG384A), but without additivity in DnaK-assisted firefly luciferase refolding, table 1. 
|
PMID:21656889
|
 
|
|-
|
Cd35
|
 
|
35-residue C-terminal truncation
|
 
|
Cd35 showed a 7-fold increase in initial
refolding rate for denatured luciferase and resulted in a 5-fold increase in
maximal luminescence as compared to wild type DnaK, Table 3.
|
PMID:20381501
|
 
|
|-
|
''dnaK7''
|
 
|
 
|
revertant
|
Mutant is Leu<sup>-</sup>. When Leu<sup>-</sup>auxotrophs of Escherichia coli after UV irradiation, were grown at temperatures between 30 and 47C the frequent of UV-induced mutation from Leu<sup>-</sup> to Leu<sup>+</sup> increased as UV dose and temperature increased. Figure 2.
|
PMID:22270474
|
 
|
 
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Q4e04ba002c7bc"  class=" tableEdit Genetic_interaction_table dataTable" 
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW0013
JW0013
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 162: Line 477:


Primer 2:CCTTTTTTGTCTTTGACTTCTTC  
Primer 2:CCTTTTTTGTCTTTGACTTCTTC  
|-
|-
|
[[:Category:Kohara_Phage:9E4|9E4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=101 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:6H3|6H3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=102 Genobase]
|
PMID:3038334
|-  
|
|
[[thrA:Gene|thrA34::Tn10]]
[[thrA:Gene|thrA34::Tn10]]
Line 170: Line 503:
CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/>
CAG18442 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38985 CGSC7320]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 66% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 66% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:thr-34::Tn10  
Synonyms:thr-34::Tn10  
|-
|-  
|
|
[[carB:Gene|carB96::Tn10]]
[[carB:Gene|carB96::Tn10]]
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CAG12093 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38008 CGSC7322]<ref name='LIB:cgsc'/>
CAG12093 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38008 CGSC7322]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 48% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:car-96::Tn10


Synonyms:car-96::Tn10
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===Notes===
===Notes===
 
Sugimoto et al <ref name='PMID:18434193'/> constructed a strain carrying a ''dnaK'' deletion and the [[lambda DE3]] prophage, which expresses T7 RNA polymerase under lac control.


==Accessions in Other Databases==
==Accessions in Other Databases==
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10241 EcoCyc:EG10241]
[[EcoCyc]]
|
|
EcoCyc:EG10241
|-
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0237 EchoBASE:EB0237]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0014 EcoliGenExpDB:b0014] 
EcoGene:EG10241
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
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[http://BioCyc.org/ECOL199310/new-image?object=C0019 ECOL199310CYC:C0019] 
RegulonDB:ECK120000235
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10241 EcoGene:EG10241]
[http://www.ncbi.nlm.nih.gov/gene?term=944750 GeneID:944750]
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Escherichia coli str. K-12 substr. MG1655
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
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[http://BioCyc.org/ECOO157/new-image?object=DNAK ECOO157CYC:DNAK] 
EchoBASE:EB0237
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/844 CGSC:844] 
ASAP:ABE-0000052
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:dna genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:36, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaK

Mnemonic

DNA

Synonyms

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.26 minutes 

MG1655: 12163..14079
<gbrowseImage> name=NC_000913:12163..14079 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 12161..14077
<gbrowseImage> name=NC_012967:12161..14077 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 12163..14079
<gbrowseImage> name=NC_012759:12163..14079 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 12163..14079
<gbrowseImage> name=NC_007779:12163..14079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 12163..14079
<gbrowseImage> name=NC_010473:12163..14079 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

12166

Edman degradation

PMID:1396676[3]
PMID:7783627[4]
PMID:9298646[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dnaK(del) (Keio:JW0013)

deletion

deletion

PMID:16738554[6]

Shigen

CGSC8342[7]

dnaKG32D

G32D

In SEG-1 and dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKV436I

V436I

In mutant SEG-2; confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG455D

G455D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG468D

G468D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaK756(ts)

temperature sensitive

CGSC:6707

dnaK(del)734::kan

deletion

deletion

PMID:16738554[6]

CGSC:100628

dnaK

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484[8]

fig 2

DnaKG384A

Glycine to Alanine at position 384.

There is a 13 fold decrease rate for substrate release in ATP bound state and increase in substrate affinity. with an overall 2.5 fold higher chaperon activity, Table 1.

PMID:21656889[9]

G384A/Cd35

Double mutant

The double mutant combines accelerated peptide binding in its ADP-bound state (like Cd35) and slower peptide release in its ATP-bound state (likeG384A), but without additivity in DnaK-assisted firefly luciferase refolding, table 1.

PMID:21656889[9]

Cd35

35-residue C-terminal truncation

Cd35 showed a 7-fold increase in initial refolding rate for denatured luciferase and resulted in a 5-fold increase in maximal luminescence as compared to wild type DnaK, Table 3.

PMID:20381501[10]

dnaK7

revertant

Mutant is Leu-. When Leu-auxotrophs of Escherichia coli after UV irradiation, were grown at temperatures between 30 and 47C the frequent of UV-induced mutation from Leu- to Leu+ increased as UV dose and temperature increased. Figure 2.

PMID:22270474[11]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0013

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCGGTAAAATAATTGGTATCGA

Primer 2:CCTTTTTTGTCTTTGACTTCTTC

9E4

Kohara Phage

Genobase

PMID:3038334[13]

6H3

Kohara Phage

Genobase

PMID:3038334[13]

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[7]

est. P1 cotransduction: 66% [14]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[7]

est. P1 cotransduction: 48% [14]
Synonyms:car-96::Tn10

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Notes

Sugimoto et al [15] constructed a strain carrying a dnaK deletion and the lambda DE3 prophage, which expresses T7 RNA polymerase under lac control.

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944750

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Schmid, D et al. (1992) Precursor of mitochondrial aspartate aminotransferase synthesized in Escherichia coli is complexed with heat-shock protein DnaK. Eur. J. Biochem. 208 699-704 PubMed
  4. ↑ Freestone, P et al. (1995) Identification of phosphoproteins in Escherichia coli. Mol. Microbiol. 15 573-80 PubMed
  5. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. ↑ 6.0 6.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ 7.0 7.1 7.2 CGSC: The Coli Genetics Stock Center
  8. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  9. ↑ 9.0 9.1 Schweizer, RS et al. (2011) Fine tuning of a biological machine: DnaK gains improved chaperone activity by altered allosteric communication and substrate binding. Chembiochem 12 1559-73 PubMed
  10. ↑ Aponte, RA et al. (2010) Directed evolution of the DnaK chaperone: mutations in the lid domain result in enhanced chaperone activity. J. Mol. Biol. 399 154-67 PubMed
  11. ↑ Saha, S & Basu, T (2012) Increase in UV mutagenesis by heat stress on UV-irradiated E. coli cells. Biochem. Genet. 50 484-93 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  15. ↑ Sugimoto, S et al. (2008) Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins. Protein Expr. Purif. 60 31-6 PubMed

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