yeaK:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

yeaK

Gene Synonym(s)

ECK1785, b1787, JW1776[1], JW1776

Product Desc.

conserved protein[2][3]

Function unknown, homologous to YbaK and an insertion domain in ProS; may deacylate mischarged tRNA[4]

Product Synonyms(s)

conserved protein[1], B1787[2][1], YeaK[2][1] , ECK1785, JW1776, b1787

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yeaK[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yeaK

Mnemonic

Systematic nomenclature

Synonyms

ECK1785, b1787, JW1776[1], JW1776

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

40.34 minutes 

MG1655: 1871598..1872101
<gbrowseImage> name=NC_000913:1871598..1872101 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1850803..1851306
<gbrowseImage> name=NC_012967:1850803..1851306 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1763657..1764160
<gbrowseImage> name=NC_012759:1763657..1764160 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1875288..1875791
<gbrowseImage> name=NC_007779:1875288..1875791 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1962169..1962672
<gbrowseImage> name=NC_010473:1962169..1962672 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyeaK (Keio:JW1776)

deletion

deletion

PMID:16738554

Shigen
CGSC9490[5]

ΔyeaK784::kan

PMID:16738554

CGSC:104382


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1776

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACTGAAATGGCTAAAGGAAG

Primer 2:CCgGCAGTGCGGCGGAAATTGAC

9F2

Kohara Phage

Genobase

PMID:3038334

7F2

Kohara Phage

Genobase

PMID:3038334

zea-225::Tn10

Linked marker

CAG18465 = CGSC7388[5]

est. P1 cotransduction: 94% [6]
Synonyms:zdj-225::Tn10

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 5% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6973

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13497

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003560

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946303

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3270

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005945

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

YeaK

Synonyms

conserved protein[1], B1787[2][1], YeaK[2][1] , ECK1785, JW1776, b1787

Product description

conserved protein[2][3]

Function unknown, homologous to YbaK and an insertion domain in ProS; may deacylate mischarged tRNA[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699

Experiment(s):EBI-1140807

Protein

yahG

PMID:16606699

Experiment(s):EBI-1140807

Protein

nadE

PMID:16606699

Experiment(s):EBI-1140807

Protein

pepD

PMID:16606699

Experiment(s):EBI-1140807

Protein

sohB

PMID:16606699

Experiment(s):EBI-1140807

Protein

mreB

PMID:16606699

Experiment(s):EBI-1140807

Protein

iscS

PMID:16606699

Experiment(s):EBI-1140807

Protein

ycjG

PMID:16606699

Experiment(s):EBI-1140807

Protein

prs

PMID:16606699

Experiment(s):EBI-1140807

Protein

tdh

PMID:16606699

Experiment(s):EBI-1140807

Protein

rsuA

PMID:16606699

Experiment(s):EBI-1140807

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

ynjE

PMID:19402753

LCMS(ID Probability):99.4

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTEMAKGSVT HQRLIALLSQ EGADFRVVTH EAVGKCEAVS EIRGTALGQG AKALVCKVKG
NGVNQHVLAI LAADQQADLS QLASHIGGLR ASLASPAEVD ELTGCVFGAI PPFSFHPKLK
LVADPLLFER FDEIAFNAGM LDKSVILKTA DYLRIAQPEL VNFRRTA
Length

167

Mol. Wt

17.85 kDa

pI

7.0 (calculated)

Extinction coefficient

1,490 - 1,865 (calc based on 1 Y, 0 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

30..155

PF04073 YbaK / prolyl-tRNA synthetases associated domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=yeaK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129741

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946303

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005945

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P64483

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6973

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13497

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946303

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3270

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

747

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

565

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

648

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yeaK

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1871578..1871618 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1787 (EcoliWiki Page)

NCBI GEO profiles for yeaK

microarray

GenExpDB:b1787 (EcoliWiki Page)

Summary of data for yeaK from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to yeaK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G6973

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3270

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1787

EcoGene

EcoGene:EG13497

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003560

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005945

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

YEAK

From SHIGELLACYC

E. coli O157

YEAK

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:55826

Pfam (EcoliWiki Page)

PF04073 YbaK / prolyl-tRNA synthetases associated domain

EcoCyc

EcoCyc:G6973

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13497

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3270

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005945

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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