yciN:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yciN

Mnemonic

Systematic nomenclature

Synonyms

ECK1267, b1273, JW1265[1], JW1265

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

28.63 minutes 

MG1655: 1328692..1328441
<gbrowseImage> name=NC_000913:1328441..1328692 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1329040..1328789
<gbrowseImage> name=NC_012967:1328789..1329040 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1219290..1219541
<gbrowseImage> name=NC_012759:1219290..1219541 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1332382..1332131
<gbrowseImage> name=NC_007779:1332131..1332382 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1418088..1417837
<gbrowseImage> name=NC_010473:1417837..1418088 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1328441

Edman degradation

PMID:9868784[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyciN (Keio:JW1265)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9140[4]

yciN::Tn5KAN-I-SceI (FB20280)

Insertion at nt 98 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20280

contains pKD46

ΔyciN::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938[6]

ΔyciN781::kan

PMID:16738554[3]

CGSC:104068


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1265

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAACAAAGAGACACAACCCAT

Primer 2:CCATCAACCAGATGATATTTTTC

4F1

Kohara Phage

Genobase

PMID:3038334[8]

13F9

Kohara Phage

Genobase

PMID:3038334[8]

trpC83::Tn10

Linked marker

CAG18455 = CGSC7371[4]

est. P1 cotransduction: 69% [9]
Synonyms:trpB83::Tn10

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[4]

est. P1 cotransduction: 18% [9]
Synonyms:zci-233::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12868

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12868

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002411

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945860

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2709

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004269

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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