xerD:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

XerD

Synonyms

site-specific tyrosine recombinase[1], B2894[2][1], XprB[2][1], XerD[2][1] , ECK2889, JW2862, xprB, b2894

Product description

site-specific recombinase[2][3];

Component of Xer site-specific recombination system[3]

Tyrosine recombinase XerCD resolves chromosome dimers; site-specific[4]

EC number (for enzymes)


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Notes

XerD binds to the dif right half site[5].

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002104

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004107

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:8402918[5]

IDA: Inferred from Direct Assay

F

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013762

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01807

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01807

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002104

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013762

P

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006313

transposition, DNA-mediated

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01807

P

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

P

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0007059

chromosome segregation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01807

P

Seeded from EcoCyc (v14.0)

complete

GO:0007059

chromosome segregation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

P

Seeded from EcoCyc (v14.0)

complete

GO:0007059

chromosome segregation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0159

P

Seeded from EcoCyc (v14.0)

complete

GO:0009037

tyrosine-based site-specific recombinase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01807

F

Seeded from EcoCyc (v14.0)

complete

GO:0015074

DNA integration

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002104

P

Seeded from EcoCyc (v14.0)

complete

GO:0015074

DNA integration

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004107

P

Seeded from EcoCyc (v14.0)

complete

GO:0015074

DNA integration

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

P

Seeded from EcoCyc (v14.0)

complete

GO:0015074

DNA integration

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013762

P

Seeded from EcoCyc (v14.0)

complete

GO:0015074

DNA integration

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0229

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011932

P

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0132

P

Seeded from EcoCyc (v14.0)

complete

GO:0048476

Holliday junction resolvase complex

PMID:11114887[6]

IDA: Inferred from Direct Assay

C

with XerC

complete

GO:0006276

plasmid maintenance

PMID:8195072[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009009

site-specific recombinase activity

PMID:11114887[6]

IDA: Inferred from Direct Assay

F

with XerC and FtsK

complete

GO:0009009

site-specific recombinase activity

PMID:8402918[5]

IDA: Inferred from Direct Assay

F

of cer and dif

complete

GO:0009037

tyrosine-based site-specific recombinase activity

PMID:8402918[5]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0071139

resolution of recombination intermediates

PMID:7746851[8]

IDA: Inferred from Direct Assay

P

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011010

F

Seeded from EcoCyc (v14.0)

complete

GO:0048476

Holliday junction resolvase complex

PMID:7746851[8]

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Xer site-specific recombination system

could be indirect

Protein

rplQ

PMID:16606699[9]

Experiment(s):EBI-1144212

Protein

ytfN

PMID:16606699[9]

Experiment(s):EBI-1144212

Protein

rpsI

PMID:16606699[9]

Experiment(s):EBI-1144212

Protein

prs

PMID:16606699[9]

Experiment(s):EBI-1144212

Protein

acpP

PMID:15690043[10]

Experiment(s):EBI-884705

Protein

mukB

PMID:15690043[10]

Experiment(s):EBI-884705

Protein

acpP

PMID:19402753[11]

LCMS(ID Probability):99.6

Protein

mukB

PMID:19402753[11]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKQDLARIEQ FLDALWLEKN LAENTLNAYR RDLSMMVEWL HHRGLTLATA QSDDLQALLA
ERLEGGYKAT SSARLLSAVR RLFQYLYREK FREDDPSAHL ASPKLPQRLP KDLSEAQVER
LLQAPLIDQP LELRDKAMLE VLYATGLRVS ELVGLTMSDI SLRQGVVRVI GKGNKERLVP
LGEEAVYWLE TYLEHGRPWL LNGVSIDVLF PSQRAQQMTR QTFWHRIKHY AVLAGIDSEK
LSPHVLRHAF ATHLLNHGAD LRVVQMLLGH SDLSTTQIYT HVATERLRQL HQQHHPRA
Length

298

Mol. Wt

34.246 kDa

pI

9.1 (calculated)

Extinction coefficient

40,910 (calc based on 9 Y, 5 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

8..90

PF02899 Phage integrase, N-terminal SAM-like domain

PMID:19920124[12]

Domain

111..286

PF00589 Phage integrase family

PMID:19920124[12]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=xerD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Mutants that are still able to bind recombination sites, but do not promote recombination at cer or in vivo plasmid resolution

  • Presumptive active site tyrosine nucleophile mutant = XerDY279F[13][14]
  • Conserved Domain II Arginine implicated in DNA phosphodiester activation = XerDR247Q[15][14]

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130796

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947362

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009500

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8P8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11071

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11071

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947362

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001060

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1064

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Blakely, G et al. (1993) Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12. Cell 75 351-61 PubMed
  6. ↑ 6.0 6.1 Barre, FX et al. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14 2976-88 PubMed
  7. ↑ Cornet, F et al. (1994) Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J. Bacteriol. 176 3188-95 PubMed
  8. ↑ 8.0 8.1 Sherratt, DJ et al. (1995) Site-specific recombination and circular chromosome segregation. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 347 37-42 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ 10.0 10.1 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  11. ↑ 11.0 11.1 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  12. ↑ 12.0 12.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
  13. ↑ Pargellis, CA et al. (1988) Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine. J. Biol. Chem. 263 7678-85 PubMed
  14. ↑ 14.0 14.1 Leslie, NR & Sherratt, DJ (1995) Site-specific recombination in the replication terminus region of Escherichia coli: functional replacement of dif. EMBO J. 14 1561-70 PubMed
  15. ↑ Parsons, RL et al. (1988) Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination. Mol. Cell. Biol. 8 3303-10 PubMed

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