PMID:8195072
| Citation |
Cornet, F, Mortier, I, Patte, J and Louarn, JM (1994) Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J. Bacteriol. 176:3188-95 |
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| Abstract |
Plasmid pSC101 harbors a 28-bp sequence which is homologous to dif, the target site of the XerC/XerD-dependent recombination system in Escherichia coli. Using a technique which allows very sensitive detection of plasmid loss, we show that recombination at this site, termed psi for pSC101 stabilized inheritance, causes a moderate increase in pSC101 stability. The role of the psi sequence in site-specific recombination has been explored in two other contexts. It was cloned in a derivative of plasmid p15A and inserted into the chromosome in place of dif. In the first situation, psi activity requires accessory sequences and results in multimer resolution; in the second situation, it suppresses the effects of the dif deletion and can promote intermolecular exchanges. Thus, psi is a site whose recombinational activity depends on the context, the first in the cer/dif family known to exhibit such flexibility. |
| Links | |
| Keywords |
Base Sequence; Chromosomes, Bacterial; Cloning, Molecular; Escherichia coli/genetics; Genetic Complementation Test; Molecular Sequence Data; Phenotype; Plasmids/genetics; Recombination, Genetic; Repetitive Sequences, Nucleic Acid; Selection, Genetic |
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