xerC:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
XerC |
|---|---|
| Synonyms |
site-specific tyrosine recombinase[1], B3811[2][1], XerC[2][1] , ECK3806, JW3784, b3811 |
| Product description |
site-specific recombinase, acts on cer sequence of ColE1, effects chromosome segregation at cell division[2][3]; Component of Xer site-specific recombination system[3] Tyrosine recombinase XerCD resolves chromosome dimers; site-specific[4] |
| EC number (for enzymes) |
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| edit table |
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Notes
XerC binds to the dif left half site[5].
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0042150 |
plasmid recombination |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009009 |
site-specific recombinase activity |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006313 |
transposition, DNA-mediated |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007049 |
cell cycle |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007049 |
cell cycle |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009037 |
tyrosine-based site-specific recombinase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015074 |
DNA integration |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015074 |
DNA integration |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015074 |
DNA integration |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015074 |
DNA integration |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015074 |
DNA integration |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051301 |
cell division |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051301 |
cell division |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009009 |
site-specific recombinase activity |
IDA: Inferred from Direct Assay |
F |
with XerD and FtsK |
complete | |||
| GO:0048476 |
Holliday junction resolvase complex |
IDA: Inferred from Direct Assay |
C |
with XerD |
complete | |||
| GO:0006276 |
plasmid maintenance |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0003677 |
DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0009037 |
tyrosine-based site-specific recombinase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006276 |
plasmid maintenance |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0071139 |
resolution of recombination intermediates |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0048476 |
Holliday junction resolvase complex |
IDA: Inferred from Direct Assay |
C |
with XerD |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of Xer site-specific recombination system |
could be indirect |
||
|
Protein |
pldA |
Experiment(s):EBI-1146722 | ||
|
Protein |
cysD |
Experiment(s):EBI-1146722 | ||
|
Protein |
prs |
Experiment(s):EBI-1146722 | ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MTDLHTDVER YLRYLSVERQ LSPITLLNYQ RQLEAIINFA SENGLQSWQQ CDVTMVRNFA VRSRRKGLGA ASLALRLSAL RSFFDWLVSQ NELKANPAKG VSAPKAPRHL PKNIDVDDMN RLLDIDINDP LAVRDRAMLE VMYGAGLRLS ELVGLDIKHL DLESGEVWVM GKGSKERRLP IGRNAVAWIE HWLDLRDLFG SEDDALFLSK LGKRISARNV QKRFAEWGIK QGLNNHVHPH KLRHSFATHM LESSGDLRGV QELLGHANLS TTQIYTHLDF QHLASVYDAA HPRAKRGK |
| Length |
298 |
| Mol. Wt |
33.868 kDa |
| pI |
9.8 (calculated) |
| Extinction coefficient |
41,940 - 42,065 (calc based on 6 Y, 6 W, and 1 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Mutants that are still able to bind recombination sites, but do not promote recombination at cer or in vivo plasmid resolution
- Presumptive active site tyrosine nucleophile mutant = XerC275F[12][13]
- Conserved Domain II Arginine implicated in DNA phosphodiester activation = XerCR243Q[14][13]
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 Blakely, G et al. (1993) Two related recombinases are required for site-specific recombination at dif and cer in E. coli K12. Cell 75 351-61 PubMed
- ↑ 6.0 6.1 6.2 Colloms, SD et al. (1990) Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. J. Bacteriol. 172 6973-80 PubMed
- ↑ 7.0 7.1 Barre, FX et al. (2000) FtsK functions in the processing of a Holliday junction intermediate during bacterial chromosome segregation. Genes Dev. 14 2976-88 PubMed
- ↑ Cornet, F et al. (1994) Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J. Bacteriol. 176 3188-95 PubMed
- ↑ 9.0 9.1 Sherratt, DJ et al. (1995) Site-specific recombination and circular chromosome segregation. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 347 37-42 PubMed
- ↑ 10.0 10.1 10.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 11.0 11.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
- ↑ Pargellis, CA et al. (1988) Suicide recombination substrates yield covalent lambda integrase-DNA complexes and lead to identification of the active site tyrosine. J. Biol. Chem. 263 7678-85 PubMed
- ↑ 13.0 13.1 Leslie, NR & Sherratt, DJ (1995) Site-specific recombination in the replication terminus region of Escherichia coli: functional replacement of dif. EMBO J. 14 1561-70 PubMed
- ↑ Parsons, RL et al. (1988) Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination. Mol. Cell. Biol. 8 3303-10 PubMed
Categories
- GO:0003677 ! DNA binding
- GO:0042150 ! plasmid recombination
- GO:0009009 ! site-specific recombinase activity
- GO:0005737 ! cytoplasm
- GO:0006313 ! transposition, DNA-mediated
- GO:0007049 ! cell cycle
- GO:0007059 ! chromosome segregation
- GO:0009037 ! tyrosine-based site-specific recombinase activity
- GO:0015074 ! DNA integration
- GO:0051301 ! cell division
- GO:0048476 ! Holliday junction resolvase complex
- GO:0006276 ! plasmid maintenance
- GO:0071139 ! resolution of recombination intermediates
- Proteins
