PMID:2254268
| Citation |
Colloms, SD, Sykora, P, Szatmari, G and Sherratt, DJ (1990) Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases. J. Bacteriol. 172:6973-80 |
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| Abstract |
Site-specific recombination at the plasmid ColE1 cer site requires the Escherichia coli chromosomal gene xerC. The xerC gene has been localized to the 85-min region of the E. coli chromosome, between cya and uvrD. The nucleotide sequences of the xerC gene and flanking regions have been determined. The xerC gene encodes a protein with a calculated molecular mass of 33.8 kDa. This protein has substantial sequence similarity to the lambda integrase family of site-specific recombinases and is probably the cer recombinase. The xerC gene is expressed as part of a multicistronic unit that includes the dapF gene and two other open reading frames. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacteriocin Plasmids/genetics; Base Sequence; Cloning, Molecular; DNA Nucleotidyltransferases/genetics; Escherichia coli/genetics; Genes, Bacterial; Genetic Complementation Test; Integrases; Molecular Sequence Data; Multigene Family; Operon; Recombination, Genetic; Restriction Mapping |
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