uvrB:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
uvrB |
---|---|
Gene Synonym(s) | |
Product Desc. |
DNA repair; excision nuclease subunit B[2][3]; Component of UvrABC Nucleotide Excision Repair Complex[2][3] Excision nuclease subunit B, ATPase I and helicase II; repair of UV damage to DNA[4] |
Product Synonyms(s) |
excinulease of nucleotide excision repair, DNA damage recognition component[1], B0779[2][1], VisB[2][1], UvrB[2][1] , dar-1, dar-6, ECK0768, JW0762, b0779 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
uvrB |
---|---|
Mnemonic |
UV resistant |
Synonyms | |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
17.52 minutes |
MG1655: 812749..814770 |
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NC_012967: 806152..808173 |
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NC_012759: 715717..717738 |
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W3110 |
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W3110: 813948..815969 |
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DH10B: 866677..868698 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
812752 |
Edman degradation |
PMID:3515321 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
uvrB(del) (Keio:JW0762) |
deletion |
deletion |
PMID:16738554 |
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uvrBY95+Y96 |
Y95A and Y96A |
Altered enzymatic activity |
Defective in DNA-unwinding activity |
PMID:11689453 |
seeded from UniProt:P0A8F8 | ||
uvrBY101+F108 |
Y101A and F108A |
Altered enzyme activity |
Defective in DNA-unwinding activity |
PMID:11689453 |
seeded from UniProt:P0A8F8 | ||
uvrB45 |
Sensitivity to |
Mutant increases in sensitivity after high UV-radiation exposure |
PMID:5335128 |
Figure 5 Strain: Hfr Hayes | |||
uvrB16 |
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uvrB5 |
PMID:6224549 |
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uvrB27 |
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uvrB29 |
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uvrB30 |
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uvrB501 |
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uvrB151 |
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uvrB153 |
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uvrB230 |
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uvrB751(del)::kan |
PMID:16738554 |
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uvrB5 |
deletion |
deletion |
Sensitivity to |
Deletion of uvrB caused hypersensitivity to PUVA (UVA-A light) treatments performed with both mono-functional 3-carbethoxypsoralen and bifunctional 8-Monofunctional psoralens when compared to wildtype, figure 1 & 2. |
PMID:20004108 |
parent strain: AB1157 |
|
uvrB(del) |
Deletion |
Deletion |
Sensitivity to |
Gene deletion enhances SOS chromatest assay sensitivity for nitroreductases which can be used for CB1954-mediated gene therapy. |
PMID:20727918 |
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uvrB301(del) |
complete null mutant |
complete null mutant |
Sensitivity to |
Deletion mutants are more sensitive to DNA damaging agent Mitomycin C, |
PMID:17040104 |
Experimental strain: JC3890 |
Figures 1 & 2 illustrate experimental results. |
uvrB5 |
point mutation |
Sensitivity to |
truncation mutation was more sensitive to lower mitomycin C concentrations than complete null mutation, uvrB301(del), |
PMID:17040104 |
See figure 2 for full experimental results. | ||
uvrB(del) |
deletion |
deletion |
Sensitivity to |
Deletion mutation causes hypersensitive to UV when irradiated and incubated in non-photoreactivating conditions, fig 2. |
PMID:16970815 |
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uvrB5 |
point mutation |
DNA repair |
truncation mutation resulted in increasing amounts of DNA strand breaks. |
PMID:17040104 |
See figure 4 for results. | ||
uvrBK45A |
Lysine to alanine |
change in enzyme activity |
ATP hydrolysis by uvrB is inhibited. |
PMID:17630776 |
Table 1. | ||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0762 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAGTAAACCGTTCAAACTGAA Primer 2:CCCGATGCCGCGATAAACAGCTC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 26% [6] | ||
zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: 75% [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001051 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB1055 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002658 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
UvrB |
---|---|
Synonyms |
excinulease of nucleotide excision repair, DNA damage recognition component[1], B0779[2][1], VisB[2][1], UvrB[2][1] , dar-1, dar-6, ECK0768, JW0762, b0779 |
Product description |
DNA repair; excision nuclease subunit B[2][3]; Component of UvrABC Nucleotide Excision Repair Complex[2][3] Excision nuclease subunit B, ATPase I and helicase II; repair of UV damage to DNA[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001650 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001943 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006935 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009055 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004386 |
helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001650 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004518 |
nuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001943 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004518 |
nuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009055 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004518 |
nuclease activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0267 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001650 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006935 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0228 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006281 |
DNA repair |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0234 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006289 |
nucleotide-excision repair |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006289 |
nucleotide-excision repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001943 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006289 |
nucleotide-excision repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006289 |
nucleotide-excision repair |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR009055 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006974 |
response to DNA damage stimulus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0227 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009381 |
excinuclease ABC activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009381 |
excinuclease ABC activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009432 |
SOS response |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00204 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009432 |
SOS response |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR004807 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009432 |
SOS response |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0742 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016787 |
hydrolase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR006935 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of UvrABC Nucleotide Excision Repair Complex |
could be indirect |
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Protein |
aceF |
PMID:15690043 |
Experiment(s):EBI-880948, EBI-887304 | |
Protein |
lpdA |
PMID:15690043 |
Experiment(s):EBI-880948, EBI-887304 | |
Protein |
garK |
PMID:15690043 |
Experiment(s):EBI-880948 | |
Protein |
hns |
PMID:15690043 |
Experiment(s):EBI-887304 | |
Protein |
hupA |
PMID:15690043 |
Experiment(s):EBI-887304 | |
Protein |
hupB |
PMID:15690043 |
Experiment(s):EBI-887304 | |
Protein |
nfi |
PMID:15690043 |
Experiment(s):EBI-887304 | |
Protein |
rpmB |
PMID:15690043 |
Experiment(s):EBI-887304 | |
Protein |
hns |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
hupA |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
hupB |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
Protein |
nfi |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA NVIADLQRPT MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA YVPSSDTFIE KDASVNEHIE QMRLSATKAM LERRDVVVVA SVSAIYGLGD PDLYLKMMLH LTVGMIIDQR AILRRLAELQ YARNDQAFQR GTFRVRGEVI DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR FTIYPKTHYV TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM YRGDRARKET LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE LEKSGGDVVD QVVRPTGLLD PIIEVRPVAT QVDDLLSEIR QRAAINERVL VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI DTVERMEIIR DLRLGEFDVL VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG RAARNVNGKA ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL EFEEAAQIRD QLHQLRELFI AAS |
Length |
673 |
Mol. Wt |
76.227 kDa |
pI |
5.0 (calculated) |
Extinction coefficient |
34,270 - 34,395 (calc based on 23 Y, 0 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0002658 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120001051 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1055 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
Ecoli K-12 |
11.53+/-0.084 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.036615537 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
370 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
197 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
292 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:812729..812769
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for uvrB | |
microarray |
Summary of data for uvrB from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
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Notes
Accessions Related to uvrB Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1055 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001051 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002658 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
UVRB |
From SHIGELLACYC |
E. coli O157 |
UVRB |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG11062 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120001051 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB1055 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0002658 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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