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Product(s) |

Expression|

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Standard Name	uidA
Gene Synonym(s)	ECK1612, b1617, JW1609, gurA, gusA, bglR, vidA^[1]
Product Desc.	BETA- beta-D-Glucuronidase^[2]
Product Synonyms(s)	beta-D-glucuronidase^[1], B1617^[3]^[1], VidA^[3]^[1], GusA^[3]^[1], GurA^[3]^[1], BglR^[3]^[1], UidA^[3]^[1], β-D-glucuronoside glucuronosohydrolase^[3]^[1] , ECK1612, gurA, gusA, JW1609, b1617
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): uidABC^[3]
Regulation/Activity
Quick Links
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Notes

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UidA has an Ig-like domain: pfam PF00703 aligns to residues 182-272. ebgA, uidA and lacZ are paralogs.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	uidA
Mnemonic	hexUronIDes
Synonyms	ECK1612, b1617, JW1609, gurA, gusA, bglR, vidA^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	36.47 minutes	MG1655: 1694095..1692284 <gbrowseImage> name=NC_000913:1692284..1694095 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1673266..1671455 <gbrowseImage> name=NC_012967:1671455..1673266 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1584343..1586154 <gbrowseImage> name=NC_012759:1584343..1586154 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1697785..1695974 <gbrowseImage> name=NC_007779:1695974..1697785 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1785056..1783245 <gbrowseImage> name=NC_010473:1783245..1785056 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1692284	Edman degradation	PMID:3105604 PMID:3534890
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔuidA (Keio:JW1609)	deletion	deletion	PMID:16738554	Shigen CGSC9370^[4]
uidA1				CGSC:5807
uidA2				CGSC:11731
ΔuidA3::pir⁺				CGSC:64688
ΔuidA4::pir-116			PMID:11591683	CGSC:70690
ΔuidA5			PMID:8335256	CGSC:91488
ΔuidA765::kan			PMID:16738554	CGSC:103298
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1609	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTACGTCCTGTAGAAACCCC Primer 2:CCTTGTTTGCCTCCCTGCTGCGG
6A5	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[4]	est. P1 cotransduction: % ^[5] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[4]	est. P1 cotransduction: % ^[5] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11055	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11055	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001044	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946149	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1048	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005410	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	UidA
Synonyms	beta-D-glucuronidase^[1], B1617^[3]^[1], VidA^[3]^[1], GusA^[3]^[1], GurA^[3]^[1], BglR^[3]^[1], UidA^[3]^[1], β-D-glucuronoside glucuronosohydrolase^[3]^[1] , ECK1612, gurA, gusA, JW1609, b1617
Product description	BETA- beta-D-Glucuronidase^[2]
EC number (for enzymes)	3.2.1.31^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004566	beta-glucuronidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.2.1.31	F	Seeded from EcoCyc (v14.0)	complete
	GO:0043169	cation binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013781	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	nadE		PMID:16606699	Experiment(s):EBI-1140287
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLRPVETPTR EIKKLDGLWA FSLDRENCGI DQRWWESALQ ESRAIAVPGS FNDQFADADI RNYAGNVWYQ REVFIPKGWA GQRIVLRFDA VTHYGKVWVN NQEVMEHQGG YTPFEADVTP YVIAGKSVRI TVCVNNELNW QTIPPGMVIT DENGKKKQSY FHDFFNYAGI HRSVMLYTTP NTWVDDITVV THVAQDCNHA SVDWQVVANG DVSVELRDAD QQVVATGQGT SGTLQVVNPH LWQPGEGYLY ELCVTAKSQT ECDIYPLRVG IRSVAVKGEQ FLINHKPFYF TGFGRHEDAD LRGKGFDNVL MVHDHALMDW IGANSYRTSH YPYAEEMLDW ADEHGIVVID ETAAVGFNLS LGIGFEAGNK PKELYSEEAV NGETQQAHLQ AIKELIARDK NHPSVVMWSI ANEPDTRPQG AREYFAPLAE ATRKLDPTRP ITCVNVMFCD AHTDTISDLF DVLCLNRYYG WYVQSGDLET AEKVLEKELL AWQEKLHQPI IITEYGVDTL AGLHSMYTDM WSEEYQCAWL DMYHRVFDRV SAVVGEQVWN FADFATSQGI LRVGGNKKGI FTRDRKPKSA AFLLQKRWTG MNFGEKPQQG GKQ
Length	603
Mol. Wt	68.445 kDa
pI	5.2 (calculated)
Extinction coefficient	140,260 - 141,385 (calc based on 24 Y, 19 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	182..272	PF00703 domain	PMID:19920124
Domain	274..593	PF02836 Glycosyl hydrolases family 2, TIM barrel domain	PMID:19920124
Domain	9..180	PF02837 Glycosyl hydrolases family 2, sugar binding domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=uidA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129575	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946149	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005410	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P05804	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11055	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11055	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946149	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001044	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1048	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	9	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	5	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
uidABC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1694075..1694115 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1617 (EcoliWiki Page)	NCBI GEO profiles for uidA
microarray	GenExpDB:b1617 (EcoliWiki Page)	Summary of data for uidA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1694010..1694501) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ03; Well:B2^[6]
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Notes

Accessions Related to uidA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11055	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1048	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1617
EcoGene	EcoGene:EG11055	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001044	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005410	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000021162 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000015174 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000000941 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00038966 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00013693 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000014967 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000010609 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0034417 (score: 1.000; bootstrap: 100%) FBgn0039874 (score: 0.390)	From Inparanoid:20070104
Drosophila pseudoobscura	GA13505-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000004002 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000302728 (score: 1.000; bootstrap: 100%) ENSP00000344495 (score: 0.265)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000027961 (score: 1.000; bootstrap: 100%) ENSMMUP00000029359 (score: 0.405)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000008075 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:95872 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000032885 (score: 1.000; bootstrap: 100%) ENSPTRP00000032859 (score: 0.207)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000045033 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000141049 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00016274001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000022145 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	UIDA	From SHIGELLACYC
E. coli O157	UIDA_1	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02836 Glycosyl hydrolases family 2, TIM barrel domain
Pfam (EcoliWiki Page)	PF00703 domain
Pfam (EcoliWiki Page)	PF02837 Glycosyl hydrolases family 2, sugar binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:49303
Superfamily (EcoliWiki Page)	SUPERFAMILY:49785
Superfamily (EcoliWiki Page)	SUPERFAMILY:51445
EcoCyc	EcoCyc:EG11055	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11055	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001044	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1048	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005410	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 ^3.13 ^3.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

uidA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to uidA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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