uidA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

uidA

Mnemonic

hexUronIDes

Synonyms

ECK1612, b1617, JW1609, gurA, gusA, bglR, vidA[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

36.47 minutes 

MG1655: 1694095..1692284
<gbrowseImage> name=NC_000913:1692284..1694095 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1673266..1671455
<gbrowseImage> name=NC_012967:1671455..1673266 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1584343..1586154
<gbrowseImage> name=NC_012759:1584343..1586154 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1697785..1695974
<gbrowseImage> name=NC_007779:1695974..1697785 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1785056..1783245
<gbrowseImage> name=NC_010473:1783245..1785056 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1692284

Edman degradation

PMID:3105604[2]
PMID:3534890[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔuidA (Keio:JW1609)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9370[5]

uidA1

CGSC:5807

uidA2

CGSC:11731

ΔuidA3::pir+

CGSC:64688

ΔuidA4::pir-116

PMID:11591683[6]

CGSC:70690

ΔuidA5

PMID:8335256[7]

CGSC:91488

ΔuidA765::kan

PMID:16738554[4]

CGSC:103298


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1609

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTACGTCCTGTAGAAACCCC

Primer 2:CCTTGTTTGCCTCCCTGCTGCGG

6A5

Kohara Phage

Genobase

PMID:3038334[9]

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[5]

est. P1 cotransduction: % [10]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: % [10]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11055

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11055

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001044

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946149

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1048

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005410

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Blanco, C & Nemoz, G (1987) One step purification of Escherichia coli beta-glucuronidase. Biochimie 69 157-61 PubMed
  3. Jefferson, RA et al. (1986) beta-Glucuronidase from Escherichia coli as a gene-fusion marker. Proc. Natl. Acad. Sci. U.S.A. 83 8447-51 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Haldimann, A & Wanner, BL (2001) Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. J. Bacteriol. 183 6384-93 PubMed
  7. Metcalf, WW & Wanner, BL (1993) Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129 17-25 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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