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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	trpC
Gene Synonym(s)	ECK1256, b1262, JW1254, trpF, trpCF^[1]^[2]
Product Desc.	PRAI-IGPS^[2]^[3] Tryptophan synthesis, bifunctional: N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthase^[4]
Product Synonyms(s)	fused indole-3-glycerolphosphate synthetase^[1], N-(5-phosphoribosyl)anthranilate isomerase^[1], B1262^[2]^[1], TrpC^[2]^[1], TrpF^[2]^[1] , ECK1256, JW1254, trpF, b1262
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): trpCBA^[2], trpLEDCBA^[2], OP00094, trp
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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The N-terminal synthase is the TrpC domain and the C-terminal isomerase domain is the TrpF domain.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	trpC
Mnemonic	Tryptophan
Synonyms	ECK1256, b1262, JW1254, trpF, trpCF^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	28.37 minutes	MG1655: 1317809..1316451 <gbrowseImage> name=NC_000913:1316451..1317809 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1317379..1316021 <gbrowseImage> name=NC_012967:1316021..1317379 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1207300..1208658 <gbrowseImage> name=NC_012759:1207300..1208658 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1321499..1320141 <gbrowseImage> name=NC_007779:1320141..1321499 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1407205..1405847 <gbrowseImage> name=NC_010473:1405847..1407205 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
trpC(del) (Keio:JW1254)	deletion	deletion	PMID:16738554	Shigen CGSC9131^[5]
trpC::Tn5KAN-2 (FB20278)	Insertion at nt 529 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20278	does not contain pKD46
trpC83::Tn10	Insertion at 1,317,596 bp in MG1655 (NC_000913)		adapted from Nichols et al.^[6]	CAG18455 = CGSC7371^[5]	Synonyms: trpB83::Tn10
trpC83::Tn10			PMID:9829956	CGSC:4709
trpC45				CGSC:4746
trpC60				CGSC:6951
trpC9999				CGSC:27412
trpC3117::Tn10kan			PMID:2540407 PMID:9829956	CGSC:39838
trpC3193::Tn10kan			PMID:2540407 PMID:9829956	CGSC:46774
trpC9800			PMID:4945859	CGSC:58648
trpC9830			PMID:4945859	CGSC:58650
trpC9941			PMID:5339605	CGSC:65101
trpC720(del)				CGSC:69381
trpC721(trpF)(del)			PMID:9605328 PMID:7556082	CGSC:69387
trpC770(del)::kan			PMID:16738554	CGSC:103226
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1254	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCAAACCGTTTTAGCGAAAAT Primer 2:CCATATGCGCGCAGCGTCTGGAA
14C4	Kohara Phage	Genobase	PMID:3038334
4F1	Kohara Phage	Genobase	PMID:3038334
oppC506::Tn10	Linked marker	CAG12169 = CGSC7369^[5]	est. P1 cotransduction: 64% ^[6] Synonyms:zch-506::Tn10, zci-506::Tn10
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zci-233::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001015	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945519	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1019	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004238	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TrpC
Synonyms	fused indole-3-glycerolphosphate synthetase^[1], N-(5-phosphoribosyl)anthranilate isomerase^[1], B1262^[2]^[1], TrpC^[2]^[1], TrpF^[2]^[1] , ECK1256, JW1254, trpF, b1262
Product description	PRAI-IGPS^[2]^[3] Tryptophan synthesis, bifunctional: N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthase^[4]
EC number (for enzymes)	5.3.1.24^[1] 4.1.1.48^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000162	tryptophan biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00134	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000162	tryptophan biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00135	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000162	tryptophan biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0822	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004425	indole-3-glycerol-phosphate synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00134	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004425	indole-3-glycerol-phosphate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001468	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004425	indole-3-glycerol-phosphate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.1.1.48	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004640	phosphoribosylanthranilate isomerase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00135	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004640	phosphoribosylanthranilate isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001240	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004640	phosphoribosylanthranilate isomerase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.3.1.24	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	groL	PMID:16606699	Experiment(s):EBI-1139357
Protein	nadE	PMID:16606699	Experiment(s):EBI-1139357
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMQTVLAKIV ADKAIWVEAR KQQQPLASFQ NEVQPSTRHF YDALQGARTA FILECKKASP SKGVIRDDFD PARIAAIYKH YASAISVLTD EKYFQGSFNF LPIVSQIAPQ PILCKDFIID PYQIYLARYY QADACLLMLS VLDDDQYRQL AAVAHSLEMG VLTEVSNEEE QERAIALGAK VVGINNRDLR DLSIDLNRTR ELAPKLGHNV TVISESGINT YAQVRELSHF ANGFLIGSAL MAHDDLHAAV RRVLLGENKV CGLTRGQDAK AAYDAGAIYG GLIFVATSPR CVNVEQAQEV MAAAPLQYVG VFRNHDIADV VDKAKVLSLA AVQLHGNEEQ LYIDTLREAL PAHVAIWKAL SVGETLPARE FQHVDKYVLD NGQGGSGQRF DWSLLNGQSL GNVLLAGGLG ADNCVEAAQT GCAGLDFNSA VESQPGIKDA RLLASVFQTL RAY
Length	453
Mol. Wt	49.492 kDa
pI	5.7 (calculated)
Extinction coefficient	40,340 - 41,215 (calc based on 16 Y, 3 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	4..252	PF00218 Indole-3-glycerol phosphate synthase	PMID:19920124
Domain	257..448	PF00697 N-(5'phosphoribosyl)anthranilate (PRA) isomerase	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=trpC taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111239	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945519	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004238	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00909	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11026	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945519	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001015	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1019	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	113	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	840	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	276	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
trpCBA
trpLEDCBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1317789..1317829 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1262 (EcoliWiki Page)	NCBI GEO profiles for trpC
microarray	GenExpDB:b1262 (EcoliWiki Page)	Summary of data for trpC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to trpC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11026	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1019	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1262
EcoGene	EcoGene:EG11026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001015	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004238	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT2G04400 (score: 1.000; bootstrap: 98%) AT5G48220 (score: 0.514)	From Inparanoid:20070104
Oryza gramene	Q6K307 (score: 1.000; bootstrap: 99%) Q6K306 (score: 0.913) Q8H3R5 (score: 0.626) Q7XJW1 (score: 0.432)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000023516 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	TRPC	From SHIGELLACYC
E. coli O157	TRPC	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00218 Indole-3-glycerol phosphate synthase
Superfamily (EcoliWiki Page)	SUPERFAMILY:51366
Superfamily (EcoliWiki Page)	SUPERFAMILY:51366
Panther (EcoliWiki Page)	PTHR22854:SF5
Pfam (EcoliWiki Page)	PF00697 N-(5'phosphoribosyl)anthranilate (PRA) isomerase
EcoCyc	EcoCyc:EG11026	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11026	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001015	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1019	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004238	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

trpC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to trpC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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