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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	tpx
Gene Synonym(s)	ECK1320, b1324, JW1317, yzzJ^[1], yzzJ
Product Desc.	thiol peroxidase 2^[2]^[3] Lipid hydroperoxide peroxidase; thiol peroxidase, antioxidant, thioredoxin-dependent; scavengase P20; induced by acid or base^[4]
Product Synonyms(s)	lipid hydroperoxide peroxidase^[1], B1324^[2]^[1], YzzJ^[2]^[1], Tpx^[2]^[1], p20^[2]^[1], scavengase^[2]^[1], scavengase p20^[2]^[1] , ECK1320, JW1317, yzzJ, b1324
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tpx^[2]
Regulation/Activity
Quick Links
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Notes

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Binds TrxA (Kumar, 2004). although initially reported to be a periplasmic enzyme, the formation of a mixed-disulfide complex with TrxA indicates a cytoplasmic localization (Tao, 2008).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tpx
Mnemonic	Thiol peroxidase
Synonyms	ECK1320, b1324, JW1317, yzzJ^[1], yzzJ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	29.88 minutes	MG1655: 1386835..1386329 <gbrowseImage> name=NC_000913:1386329..1386835 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1387180..1386674 <gbrowseImage> name=NC_012967:1386674..1387180 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1277178..1277684 <gbrowseImage> name=NC_012759:1277178..1277684 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1390525..1390019 <gbrowseImage> name=NC_007779:1390019..1390525 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1476231..1475725 <gbrowseImage> name=NC_010473:1475725..1476231 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1386332	Edman degradation	PMID:7499381 PMID:8740179 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δtpx (Keio:JW1317)	deletion	deletion	PMID:16738554	Shigen CGSC9180^[5]
tpx::Tn5KAN-2 (FB20293)	Insertion at nt 251 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20293	does not contain pKD46
Δtpx-761::kan			PMID:16738554	CGSC:103196
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1317	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCACAAACCGTTCATTTCCA Primer 2:CCTGCTTTCAGTACAGCCAGAGC
3H8	Kohara Phage	Genobase	PMID:3038334
6B4	Kohara Phage	Genobase	PMID:3038334
zcj-233::Tn10	Linked marker	CAG12028 = CGSC7373^[5]	est. P1 cotransduction: 53% ^[6] Synonyms:zci-233::Tn10
recT3061::Tn10	Linked marker	CAG12081 = CGSC7375^[5]	est. P1 cotransduction: 41% ^[6] Synonyms:zcj-3061::Tn10, zda-3061::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6660	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12672	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003255	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945880	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2538	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004442	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Tpx
Synonyms	lipid hydroperoxide peroxidase^[1], B1324^[2]^[1], YzzJ^[2]^[1], Tpx^[2]^[1], p20^[2]^[1], scavengase^[2]^[1], scavengase p20^[2]^[1] , ECK1320, JW1317, yzzJ, b1324
Product description	thiol peroxidase 2^[2]^[3] Lipid hydroperoxide peroxidase; thiol peroxidase, antioxidant, thioredoxin-dependent; scavengase P20; induced by acid or base^[4]
EC number (for enzymes)	1.11.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008379	thioredoxin peroxidase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00269	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008379	thioredoxin peroxidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002065	F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0045454	cell redox homeostasis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017936	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002065	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018219	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	garK	PMID:16606699	Experiment(s):EBI-1139555
Protein	cysB	PMID:16606699	Experiment(s):EBI-1139555
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[3]
Periplasm	PMID:7499381	EchoLocation:tpx
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSQTVHFQGN PVTVANSIPQ AGSKAQTFTL VAKDLSDVTL GQFAGKRKVL NIFPSIDTGV CAASVRKFNQ LATEIDNTVV LCISADLPFA QSRFCGAEGL NNVITLSTFR NAEFLQAYGV AIADGPLKGL AARAVVVIDE NDNVIFSQLV DEITTEPDYE AALAVLKA
Length	168
Mol. Wt	17.834 kDa
pI	4.6 (calculated)
Extinction coefficient	2,980 - 3,355 (calc based on 2 Y, 0 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A862
Modification Site	17	phosphorylation site at S17	probability greater than 75%	PMID:17938405
Domain	20..163	PF08534 Redoxin		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tpx taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129285	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945880	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004442	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A862	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6660	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12672	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945880	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003255	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2538	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.28E+04	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 EMG2	2480	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M32	PMID: 9298646
Protein	E. coli K-12 MG1655	47473	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	16961	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	30998	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tpx
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1386815..1386855 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1324 (EcoliWiki Page)	NCBI GEO profiles for tpx
microarray	GenExpDB:b1324 (EcoliWiki Page)	Summary of data for tpx from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to tpx Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6660	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2538	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1324
EcoGene	EcoGene:EG12672	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003255	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004442	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	TPX	From SHIGELLACYC
E. coli O157	TPX	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:52833
Panther (EcoliWiki Page)	PTHR10681:SF22
Pfam (EcoliWiki Page)	PF08534 Redoxin
EcoCyc	EcoCyc:G6660	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12672	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003255	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2538	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004442	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

tpx:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tpx Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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