PMID:23818486
| Citation |
Teleha, MA, Miller, AC and Larsen, RA (2013) Overexpression of the Escherichia coli TolQ protein leads to a null-FtsN-like division phenotype. Microbiologyopen 2:618-32 |
|---|---|
| Abstract |
Mutations involving the Tol-Pal complex of Escherichia coli result in a subtle phenotype in which cells chain when grown under low-salt conditions. Here, the nonpolar deletion of individual genes encoding the cytoplasmic membrane-associated components of the complex (TolQ, TolR, TolA) produced a similar phenotype. Surprisingly, the overexpression of one of these proteins, TolQ, resulted in a much more overt phenotype in which cells occurred as elongated rods coupled in long chains when grown under normal salt conditions. Neither TolR nor TolA overexpression produced a phenotype, nor was the presence of either protein required for the TolQ-dependent phenotype. Consistent with their native membrane topology, the amino-terminal domain of TolQ specifically associated in vivo with the periplasmic domain of FtsN in a cytoplasm-based two-hybrid analysis. Further, the concomitant overexpression of FtsN rescued the TolQ-dependent phenotype, suggesting a model wherein the overexpression of TolQ sequesters FtsN, depleting this essential protein from the divisome during Gram-negative cell division. The role of the Tol-Pal system in division is discussed. |
| Links |
PubMed PMC3831626 Online version:10.1002/mbo3.101 |
| Keywords |
Cell Cycle; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Deletion; Gene Expression; Membrane Proteins/genetics; Membrane Proteins/metabolism; Protein Binding; Protein Interaction Domains and Motifs; Two-Hybrid System Techniques |
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