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Standard Name	tktA
Gene Synonym(s)	ECK2930, b2935, JW5478, tkt^[1], tkt
Product Desc.	TktA^[2]^[3]; Component of transketolase I^[2]^[3] Transketolase A; binds Zn(II)^[4]
Product Synonyms(s)	transketolase 1, thiamin-binding^[1], B2935^[2]^[1], Tkt^[2]^[1], TktA^[2]^[1] , ECK2930, JW5478, tkt, b2935
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tktA^[2]
Regulation/Activity
Quick Links
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Notes

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Mutations affect chromosomal supercoliing.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tktA
Mnemonic	Transketolase
Synonyms	ECK2930, b2935, JW5478, tkt^[1], tkt
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	66.33 minutes	MG1655: 3079657..3077666 <gbrowseImage> name=NC_000913:3077666..3079657 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2967386..2965395 <gbrowseImage> name=NC_012967:2965395..2967386 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2964814..2966805 <gbrowseImage> name=NC_012759:2964814..2966805 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3080291..3078300 <gbrowseImage> name=NC_007779:3078300..3080291 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3174726..3172735 <gbrowseImage> name=NC_010473:3172735..3174726 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3077666	Edman degradation	PMID:1537815
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔtktA (Keio:JW5478)	deletion	deletion			PMID:16738554	Shigen
tktA::Tn5KAN-I-SceI (FB20981)	Insertion at nt 546 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20981	contains pKD46
tktA::Tn5KAN-I-SceI (FB20982)	Insertion at nt 546 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20982	does not contain pKD46
tktA2						CGSC:8193
tktA::Tn10 tktB::kan			Auxotrophies	Double mutant inhibit transketolates whose product is D-erythrose-4-phosphate. This product is a precursor to pyridoxine (vitamin B6), an essential vitamin for growth. Table 1.	PMID:7928977	Strain: TX3158 and AI1118
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5478	Plasmid clone	Shigen	PMID:16769691 Status:cloned as old JW Primer 1:GCCTCCTCACGTAAAGAGCTTGC Primer 2:CCaAGCAGTTCTTTTGCTTTCGC
6C5	Kohara Phage	Genobase	PMID:3038334
1H10	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: % ^[6]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[5]	est. P1 cotransduction: 77% ^[6] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001391	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947420	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1397	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009625	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TktA
Synonyms	transketolase 1, thiamin-binding^[1], B2935^[2]^[1], Tkt^[2]^[1], TktA^[2]^[1] , ECK2930, JW5478, tkt, b2935
Product description	TktA^[2]^[3]; Component of transketolase I^[2]^[3] Transketolase A; binds Zn(II)^[4]
EC number (for enzymes)	2.2.1.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	Aspect	Notes	Status
	GO:0015949	nucleobase, nucleoside and nucleotide interconversion			P	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004802	transketolase activity	PMID:7607225	IDA: Inferred from Direct Assay	F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of transketolase I	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSSRKELANA IRALSMDAVQ KAKSGHPGAP MGMADIAEVL WRDFLKHNPQ NPSWADRDRF VLSNGHGSML IYSLLHLTGY DLPMEELKNF RQLHSKTPGH PEVGYTAGVE TTTGPLGQGI ANAVGMAIAE KTLAAQFNRP GHDIVDHYTY AFMGDGCMME GISHEVCSLA GTLKLGKLIA FYDDNGISID GHVEGWFTDD TAMRFEAYGW HVIRDIDGHD AASIKRAVEE ARAVTDKPSL LMCKTIIGFG SPNKAGTHDS HGAPLGDAEI ALTREQLGWK YAPFEIPSEI YAQWDAKEAG QAKESAWNEK FAAYAKAYPQ EAAEFTRRMK GEMPSDFDAK AKEFIAKLQA NPAKIASRKA SQNAIEAFGP LLPEFLGGSA DLAPSNLTLW SGSKAINEDA AGNYIHYGVR EFGMTAIANG ISLHGGFLPY TSTFLMFVEY ARNAVRMAAL MKQRQVMVYT HDSIGLGEDG PTHQPVEQVA SLRVTPNMST WRPCDQVESA VAWKYGVERQ DGPTALILSR QNLAQQERTE EQLANIARGG YVLKDCAGQP ELIFIATGSE VELAVAAYEK LTAEGVKARV VSMPSTDAFD KQDAAYRESV LPKAVTARVA VEAGIADYWY KYVGLNGAIV GMTTFGESAP AELLFEEFGF TVDNVVAKAK ELL
Length	663
Mol. Wt	72.212 kDa
pI	5.5 (calculated)
Extinction coefficient	94,770 - 95,395 (calc based on 23 Y, 11 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	353..526	PF02779 Transketolase, pyrimidine binding domain	PMID:19920124
Domain	540..655	PF02780 Transketolase, C-terminal domain	PMID:19920124
Domain	3..334	PF00456 Transketolase, thiamine diphosphate binding domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tktA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176286	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947420	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009625	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P27302	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11427	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947420	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001391	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1397	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	5.11E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	290.091+/-2.427	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.09567+/-0.01304	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by FISH	PMID:20671182
mRNA	Ecoli K-12	0.187343044	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	13819	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	4690	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8242	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tktA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3079637..3079677 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2935 (EcoliWiki Page)	NCBI GEO profiles for tktA
microarray	GenExpDB:b2935 (EcoliWiki Page)	Summary of data for tktA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3079578..3079873) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:A11^[7]
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Notes

Accessions Related to tktA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11427	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1397	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2935
EcoGene	EcoGene:EG11427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001391	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009625	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	TKTA	From SHIGELLACYC
E. coli O157	TKTA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02779 Transketolase, pyrimidine binding domain
Pfam (EcoliWiki Page)	PF02780 Transketolase, C-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:52518
Superfamily (EcoliWiki Page)	SUPERFAMILY:52518
Superfamily (EcoliWiki Page)	SUPERFAMILY:52922
Pfam (EcoliWiki Page)	PF00456 Transketolase, thiamine diphosphate binding domain
EcoCyc	EcoCyc:EG11427	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001391	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1397	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009625	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

tktA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tktA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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