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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	thiP
Gene Synonym(s)	ECK0068, b0067, JW0066, sfuB, yabK^[1], yabK
Product Desc.	SfuB^[2]^[3]; Component of thiamin ABC transporter^[2]^[3] Thiamine and thiamine pyrophosphate ABC transporter^[4]
Product Synonyms(s)	fused thiamin transporter subunits of ABC superfamily: membrane components^[1], B0067^[2]^[1], YabK^[2]^[1], SfuB^[2]^[1], ThiP^[2]^[1] , ECK0068, JW0066, sfuB, yabK, b0067
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): thiQ^[2]
Regulation/Activity
Quick Links
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Notes

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Primarily characterized in Salmonella^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	thiP
Mnemonic	Thiamin (and thiazole)
Synonyms	ECK0068, b0067, JW0066, sfuB, yabK^[1], yabK
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.57 minutes	MG1655: 74521..72911 <gbrowseImage> name=NC_000913:72911..74521 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 77325..75715 <gbrowseImage> name=NC_012967:75715..77325 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 72910..74520 <gbrowseImage> name=NC_012759:72910..74520 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 74521..72911 <gbrowseImage> name=NC_007779:72911..74521 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
yabK::Tn5KAN-I-SceI (FB20007)	Insertion at nt 1064 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20007	contains pKD46
yabK::Tn5KAN-I-SceI (FB20008)	Insertion at nt 1064 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20008	does not contain pKD46
thiP(del) (Keio:JW0066)	deletion	deletion	PMID:16738554	Shigen CGSC8368^[5]
thiP774(del)::kan			PMID:16738554	CGSC:103144
Strain BW2952	T18C C64T A91G T205G G211A C352G T268C C283G T298C G304A C344T T409C G418A A421G A503G C535A G802T A834C G843A T880C G893A G907T C943T C958G C1009T TA1074-1075CG T1099C C1005T G1117A C1159T C1171T A1174G G1177C G1295A A1300G C1312T A1324G T1398C T1411C		PMID:19376874		Nucleotide changes in BW2952Relative to MG1655.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0066	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAACGCGCCGTCAGCCGTT Primer 2:CCGTCAGTTTTAACATTTCGCCC
5H5	Kohara Phage	Genobase	PMID:3038334
8H11	Kohara Phage	Genobase	PMID:3038334
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 18% ^[6] Synonyms:car-96::Tn10
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 69% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11573	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11573	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001525	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944784	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1533	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000246	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	ThiP
Synonyms	fused thiamin transporter subunits of ABC superfamily: membrane components^[1], B0067^[2]^[1], YabK^[2]^[1], SfuB^[2]^[1], ThiP^[2]^[1] , ECK0068, JW0066, sfuB, yabK, b0067
Product description	SfuB^[2]^[3]; Component of thiamin ABC transporter^[2]^[3] Thiamine and thiamine pyrophosphate ABC transporter^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of thiamin ABC transporter	could be indirect
Protein	ydbK		PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MATRRQPLIP GWLIPGVSAT TLVVAVALAA FLALWWNAPQ DDWVAVWQDS YLWHVVRFSF WQAFLSALLS VIPAIFLARA LYRRRFPGRL ALLRLCAMTL ILPVLVAVFG ILSVYGRQGW LATLCQSLGL EWTFSPYGLQ GILLAHVFFN LPMASRLLLQ ALENIPGEQR QLAAQLGMRS WHFFRFVEWP WLRRQIPPVA ALIFMLCFAS FATVLSLGGG PQATTIELAI YQALSYDYDP ARAAMLALLQ MVCCLGLVLL SQRLSKAIAP GTTLLQGWRD PDDRLHSRIC DTVLIVLALL LLLPPLLAVI VDGVNRQLPE VLAQPVLWQA LWTSLRIALA AGVLCVVLTM MLLWSSRELR ARQKMLAGQV LEMSGMLILA MPGIVLATGF FLLLNNTIGL PQSADGIVIF TNALMAIPYA LKVLENPMRD ITARYSMLCQ SLGIEGWSRL KVVELRALKR PLAQALAFAC VLSIGDFGVV ALFGNDDFRT LPFYLYQQIG SYRSQDGAVT ALILLLLCFL LFTVIEKLPG RNVKTD
Length	536
Mol. Wt	59.535 kDa
pI	9.5 (calculated)
Extinction coefficient	111,380 - 112,630 (calc based on 12 Y, 17 W, and 10 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	70..273	PF00528 Binding-protein-dependent transport system inner membrane component	PMID:19920124
Domain	359..532	PF00528 Binding-protein-dependent transport system inner membrane component	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=thiP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128061	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944784	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000246	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31549	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11573	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11573	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944784	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001525	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1533	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	34	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	41	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	10^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
thiQ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:74501..74541 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0067 (EcoliWiki Page)	NCBI GEO profiles for thiP
microarray	GenExpDB:b0067 (EcoliWiki Page)	Summary of data for thiP from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to thiP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11573	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1533	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0067
EcoGene	EcoGene:EG11573	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001525	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000246	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000002232 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YABK	From SHIGELLACYC
E. coli O157	YABK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00528 Binding-protein-dependent transport system inner membrane component
Pfam (EcoliWiki Page)	PF00528 Binding-protein-dependent transport system inner membrane component
EcoCyc	EcoCyc:EG11573	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11573	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001525	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1533	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000246	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

thiP:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to thiP Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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