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Expression|

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Standard Name	tatA
Gene Synonym(s)	ECK3830, b3836, JW3813, mttA1, yig, yigT^[1], yigT
Product Desc.	TatA^[2]^[3]; Component of TatABCE protein export complex^[2]^[3] Protein translocase, Sec-independent; mediates export of folded and ligand-bound proteins^[4]
Product Synonyms(s)	TatABCE protein translocation system subunit^[1], TatA^[2]^[1], YigT^[2]^[1], B3836^[2]^[1], twin-arginine translocase subunit, sec-independent protein export^[2]^[1], MttA1^[2]^[1] , ECK3830, JW3813, mttA1, yigT, b3836
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): tatA^[2]
Regulation/Activity
Quick Links
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Notes

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Forms a complex with TatBC^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	tatA
Mnemonic	Twin arginine translocation
Synonyms	ECK3830, b3836, JW3813, mttA1, yig, yigT^[1], yigT
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	86.64 minutes, 86.64 minutes	MG1655: 4019968..4020237 <gbrowseImage> name=NC_000913:4019968..4020237 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4000416..4000685 <gbrowseImage> name=NC_012967:4000416..4000685 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3909637..3909906 <gbrowseImage> name=NC_012759:3909637..3909906 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3614736..3614467 <gbrowseImage> name=NC_007779:3614467..3614736 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4118888..4119157 <gbrowseImage> name=NC_010473:4118888..4119157 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔtatA (Keio:JW3813)	deletion	deletion			PMID:16738554	Shigen CGSC10760^[5]
ΔtatA779::kan					PMID:16738554	CGSC:103092
tatA(del)			Sensitivity to	Strain carrying deletion at tatA locus which encodes a subunit of the twin argenine translocation complex (tat)used for protein secretion, exhibited increased susceptibility to protamine, Alpha helical CAMPs magainin 2 and melittin. Fig 1 and 2	PMID:21149452	Parent strain: BW25113
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3813	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGGTGGTATCAGTATTTGGCA Primer 2:CCaACCTGCTCTTTATCGTGGCG
16G1	Kohara Phage	Genobase	PMID:3038334
1C10	Kohara Phage	Genobase	PMID:3038334
metEo-3079::Tn10	Linked marker	CAG18491 = CGSC7464^[5]	est. P1 cotransduction: 68% ^[6] Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.
fad-751::Tn10	Linked marker	CAG18496 = CGSC7465^[5]	est. P1 cotransduction: 78% ^[6] Synonyms:fadAB101::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7806	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11478	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004314	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948321	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1444	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	TatA
Synonyms	TatABCE protein translocation system subunit^[1], TatA^[2]^[1], YigT^[2]^[1], B3836^[2]^[1], twin-arginine translocase subunit, sec-independent protein export^[2]^[1], MttA1^[2]^[1] , ECK3830, JW3813, mttA1, yigT, b3836
Product description	TatA^[2]^[3]; Component of TatABCE protein export complex^[2]^[3] Protein translocase, Sec-independent; mediates export of folded and ligand-bound proteins^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009306	protein secretion	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00236	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009306	protein secretion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006312	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009977	proton motive force dependent protein transmembrane transporter activity	PMID:11350936	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00236	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003369	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006312	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008320	protein transmembrane transporter activity	PMID:9649434	IGI: Inferred from Genetic Interaction	EcoliWiki:tatE	F	replaced original annotation to GO:0008565, because this term was made obsolete	complete
	GO:0015031	protein transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:12427031	IDA: Inferred from Direct Assay		C		complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006312	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:16027357	IPI: Inferred from Physical Interaction		C	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0033281	TAT protein transport complex	PMID:11279240	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of TatABCE protein export complex	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Inner membrane		PMID:14580344	EchoLocation:tatA	Membrane anchored
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLCLIIIYH RGTCMGGISI WQLLIIAVIV VLLFGTKKLG SIGSDLGASI KGFKKAMSDD EPKQDKTSQD ADFTAKTIAD KQADTNQEQA KTEDAKRHDK EQV
Length	103
Mol. Wt	11.338 kDa
pI	8.0 (calculated)
Extinction coefficient	6,990 - 7,240 (calc based on 1 Y, 1 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..83	PF02416 mttA/Hcf106 family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tatA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111653	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948321	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012541	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P69428	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7806	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11478	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948321	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004314	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1444	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	5.46E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	6336	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	2926	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	4795	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
tatA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4019948..4019988 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3836 (EcoliWiki Page)	NCBI GEO profiles for tatA
microarray	GenExpDB:b3836 (EcoliWiki Page)	Summary of data for tatA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	C-terminal 6x His tag		PMID:12427031
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Notes

Accessions Related to tatA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7806	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1444	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3836
EcoGene	EcoGene:EG11478	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004314	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000028218 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S3840	From SHIGELLACYC
E. coli O157	TATA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02416 mttA/Hcf106 family
EcoCyc	EcoCyc:G7806	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11478	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004314	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1444	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

tatA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to tatA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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