tatA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tatA

Mnemonic

Twin arginine translocation

Synonyms

ECK3830, b3836, JW3813, mttA1, yig, yigT[1], yigT

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

86.64 minutes, 86.64 minutes 

MG1655: 4019968..4020237
<gbrowseImage> name=NC_000913:4019968..4020237 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4000416..4000685
<gbrowseImage> name=NC_012967:4000416..4000685 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3909637..3909906
<gbrowseImage> name=NC_012759:3909637..3909906 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3614736..3614467
<gbrowseImage> name=NC_007779:3614467..3614736 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4118888..4119157
<gbrowseImage> name=NC_010473:4118888..4119157 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔtatA (Keio:JW3813)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10760[3]

ΔtatA779::kan

PMID:16738554[2]

CGSC:103092

tatA(del)

Sensitivity to

Strain carrying deletion at tatA locus which encodes a subunit of the twin argenine translocation complex (tat)used for protein secretion, exhibited increased susceptibility to protamine, Alpha helical CAMPs magainin 2 and melittin. Fig 1 and 2

PMID:21149452[4]

Parent strain: BW25113


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3813

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGGTGGTATCAGTATTTGGCA

Primer 2:CCaACCTGCTCTTTATCGTGGCG

16G1

Kohara Phage

Genobase

PMID:3038334[6]

1C10

Kohara Phage

Genobase

PMID:3038334[6]

metEo-3079::Tn10

Linked marker

CAG18491 = CGSC7464[3]

est. P1 cotransduction: 68% [7]
Synonyms:metE3079::Tn10 nnnThe Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met- phenotype.

fad-751::Tn10

Linked marker

CAG18496 = CGSC7465[3]

est. P1 cotransduction: 78% [7]
Synonyms:fadAB101::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7806

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11478

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004314

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948321

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1444

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012541

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Weatherspoon-Griffin, N et al. (2011) The CpxR/CpxA two-component system up-regulates two Tat-dependent peptidoglycan amidases to confer bacterial resistance to antimicrobial peptide. J. Biol. Chem. 286 5529-39 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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