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Gene |

Product(s) |

Expression|

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Standard Name	suhB
Gene Synonym(s)	ECK2530, b2533, JW2517, ssyA, G478, sfiA^[1]^[2]
Product Desc.	EG10983^[2]; inositol monophosphatase, putative mRNA turnover protein^[3] Inositol-1-monophosphatase; mutation suppresses TS growth phenotype of rpoH15, dnaB121, and secY24; suhB mutations confers CS growth^[4]
Product Synonyms(s)	inositol monophosphatase^[1], B2533^[2]^[1], SsyA^[2]^[1], SfiA^[2]^[1], SuhB^[2]^[1] , ECK2530, JW2517, ssyA, b2533
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): suhB^[2]
Regulation/Activity
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Notes

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The N-terminal sequence was determined using protein made with a synthetic RBS, and thus is not a confirmation of the in vivo start site; the N-terminal Met was not clipped off the recombinant protein.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	suhB
Mnemonic	Suppressor heat-shock proteins
Synonyms	ECK2530, b2533, JW2517, ssyA, G478, sfiA^[1]^[2]
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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	57.36 minutes	MG1655: 2661464..2662267 <gbrowseImage> name=NC_000913:2661464..2662267 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2584141..2584944 <gbrowseImage> name=NC_012967:2584141..2584944 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2547269..2548072 <gbrowseImage> name=NC_012759:2547269..2548072 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2662098..2662901 <gbrowseImage> name=NC_007779:2662098..2662901 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2753229..2754032 <gbrowseImage> name=NC_010473:2753229..2754032 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
suhB10	C547T & T746C	R183C & V249A	Growth Phenotype	Mutant is unable to grow at 25^oC.	PMID:8589060
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2517	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCATCCGATGCTGAACATCGC Primer 2:CCACGCTTCAGAGCGTCGCTTAA
5E10	Kohara Phage	Genobase	PMID:3038334
6F10	Kohara Phage	Genobase	PMID:3038334
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[5]	est. P1 cotransduction: 2% ^[6]
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 95% ^[6] Synonyms:zff-208::Tn10
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Notes

Accessions in Other Databases

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Database	Accession	Notes
EcoCyc	EcoCyc:EG10983	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10983	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000972	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947285	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0976	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008337	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	SuhB
Synonyms	inositol monophosphatase^[1], B2533^[2]^[1], SsyA^[2]^[1], SfiA^[2]^[1], SuhB^[2]^[1] , ECK2530, JW2517, ssyA, b2533
Product description	EG10983^[2]; inositol monophosphatase, putative mRNA turnover protein^[3] Inositol-1-monophosphatase; mutation suppresses TS growth phenotype of rpoH15, dnaB121, and secY24; suhB mutations confers CS growth^[4]
EC number (for enzymes)	3.1.3.25^[1]
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008934	inositol-1(or 4)-monophosphatase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.3.25	F	Seeded from EcoCyc (v14.0)	complete
	GO:0031403	lithium ion binding	PMID:10747806^[7]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:10747806^[7]	IDA: Inferred from Direct Assay		C		complete
	GO:0046872	metal ion binding	PMID:17652087^[8]	IPI: Inferred from Physical Interaction		F	CHEBI:29035	Missing: with/from
	GO:0000287	magnesium ion binding	PMID:10747806^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0008934	inositol-1(or 4)-monophosphatase activity	PMID:10747806^[7]	IDA: Inferred from Direct Assay		F		complete
	GO:0031403	lithium ion binding	PMID:10747806^[7]	IDA: Inferred from Direct Assay		F	Binding of Li²⁺ is inhibitory.	complete
	GO:0046855	inositol phosphate dephosphorylation	PMID:10747806^[7]	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rpsD	PMID:16606699^[9]	Experiment(s):EBI-1143175
Protein	nadE	PMID:16606699^[9]	Experiment(s):EBI-1143175
Protein	rplD	PMID:16606699^[9]	Experiment(s):EBI-1143175
Protein	slyD	PMID:16606699^[9]	Experiment(s):EBI-1143175
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MHPMLNIAVR AARKAGNLIA KNYETPDAVE ASQKGSNDFV TNVDKAAEAV IIDTIRKSYP QHTIITEESG ELEGTDQDVQ WVIDPLDGTT NFIKRLPHFA VSIAVRIKGR TEVAVVYDPM RNELFTATRG QGAQLNGYRL RGSTARDLDG TILATGFPFK AKQYATTYIN IVGKLFNECA DFRRTGSAAL DLAYVAAGRV DGFFEIGLRP WDFAAGELLV REAGGIVSDF TGGHNYMLTG NIVAGNPRVV KAMLANMRDE LSDALKR
Length	267
Mol. Wt	29.172 kDa
pI	6.9 (calculated)
Extinction coefficient	22,920 - 23,045 (calc based on 8 Y, 2 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..263	PF00459 Inositol monophosphatase family		PMID:19920124^[10]
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<motif_map/>

Structure
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Structures

<beststructure> gene=suhB taxon=562,83333 </beststructure>

Models

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Structure figures

Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130458	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947285	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008337	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ADG4	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10983	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10983	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947285	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000972	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0976	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	3.19E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	11711	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1591	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1767	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
suhB
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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2661444..2661484 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2533 (EcoliWiki Page)	NCBI GEO profiles for suhB
microarray	GenExpDB:b2533 (EcoliWiki Page)	Summary of data for suhB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to suhB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10983	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0976	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2533
EcoGene	EcoGene:EG10983	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000972	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008337	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000023742 (score: 1.000; bootstrap: 99%) ENSANGP00000011437 (score: 0.052)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000025568 (score: 1.000; bootstrap: 94%) ENSAPMP00000005924 (score: 0.136)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G02870 (score: 1.000; bootstrap: 61%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000015548 (score: 1.000; bootstrap: 99%) ENSBTAP00000025956 (score: 0.320)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00040566 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00008765 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000012482 (score: 1.000; bootstrap: 99%) ENSCAFP00000027751 (score: 0.414)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000002314 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040718-245 (score: 1.000; bootstrap: 99%) ZDB-CDNA-040718-273 (score: 1.000; bootstrap: 99%) ZDB-CDNA-050522-349 (score: 0.399) ZDB-GENE-050522-349 (score: 0.399)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0204100 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0037063 (score: 1.000; bootstrap: 100%) FBgn0037064 (score: 0.209) FBgn0036551 (score: 0.098) FBgn0036552 (score: 0.081) FBgn0036553 (score: 0.055)	From Inparanoid:20070104
Drosophila pseudoobscura	GA21751-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000025398 (score: 1.000; bootstrap: 99%) ENSGALP00000022380 (score: 0.473)	From Inparanoid:20070104
Homo sapiens	ENSP00000256108 (score: 1.000; bootstrap: 99%) ENSP00000269159 (score: 0.437)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000003532 (score: 1.000; bootstrap: 99%) ENSMMUP00000013725 (score: 0.403)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000007775 (score: 1.000; bootstrap: 99%) ENSMODP00000026772 (score: 0.368)	From Inparanoid:20070104
Mus musculus	MGI:2149728 (score: 1.000; bootstrap: 100%) MGI:1933158 (score: 0.422)	From Inparanoid:20070104
Oryza gramene	Q6F2U7 (score: 1.000; bootstrap: 65%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000016801 (score: 1.000; bootstrap: 99%) ENSPTRP00000034860 (score: 0.278)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000025147 (score: 1.000; bootstrap: 100%) ENSRNOP00000014274 (score: 0.418)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDR287W (score: 1.000; bootstrap: 100%) YHR046C (score: 0.410)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000144417 (score: 1.000; bootstrap: 98%) NEWSINFRUP00000144419 (score: 0.529)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00004507001 (score: 1.000; bootstrap: 100%) GSTENP00025538001 (score: 0.525) GSTENP00025537001 (score: 0.503) GSTENP00004506001 (score: 0.146)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000002482 (score: 1.000; bootstrap: 53%)	From Inparanoid:20070104
Shigella flexneri	SUHB	From SHIGELLACYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00459 Inositol monophosphatase family
Panther (EcoliWiki Page)	PTHR20854:SF10
Superfamily (EcoliWiki Page)	SUPERFAMILY:56655
EcoCyc	EcoCyc:EG10983	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10983	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000972	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0976	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008337	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^7.0 ^7.1 ^7.2 ^7.3 ^7.4 ^7.5 Chen, L & Roberts, MF (2000) Overexpression, purification, and analysis of complementation behavior of E. coli SuhB protein: comparison with bacterial and archaeal inositol monophosphatases. Biochemistry 39 4145-53 PubMed
↑ Wang, Y et al. (2007) The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications for its functional interactions in Escherichia coli. J. Biol. Chem. 282 26989-96 PubMed
↑ ^9.0 ^9.1 ^9.2 ^9.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

suhB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to suhB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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