suhB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SuhB

Synonyms

inositol monophosphatase[1], B2533[2][1], SsyA[2][1], SfiA[2][1], SuhB[2][1] , ECK2530, JW2517, ssyA, b2533

Product description

EG10983[2]; inositol monophosphatase, putative mRNA turnover protein[3]

Inositol-1-monophosphatase; mutation suppresses TS growth phenotype of rpoH15, dnaB121, and secY24; suhB mutations confers CS growth[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0008934

inositol-1(or 4)-monophosphatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.1.3.25

F

Seeded from EcoCyc (v14.0)

complete

GO:0031403

lithium ion binding

PMID:10747806[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:10747806[5]

IDA: Inferred from Direct Assay

C

complete

GO:0046872

metal ion binding

PMID:17652087[6]

IPI: Inferred from Physical Interaction

F

CHEBI:29035

Missing: with/from

GO:0000287

magnesium ion binding

PMID:10747806[5]

IDA: Inferred from Direct Assay

F

complete

GO:0008934

inositol-1(or 4)-monophosphatase activity

PMID:10747806[5]

IDA: Inferred from Direct Assay

F

complete

GO:0031403

lithium ion binding

PMID:10747806[5]

IDA: Inferred from Direct Assay

F

Binding of Li2+ is inhibitory.

complete

GO:0046855

inositol phosphate dephosphorylation

PMID:10747806[5]

IDA: Inferred from Direct Assay

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsD

PMID:16606699[7]

Experiment(s):EBI-1143175

Protein

nadE

PMID:16606699[7]

Experiment(s):EBI-1143175

Protein

rplD

PMID:16606699[7]

Experiment(s):EBI-1143175

Protein

slyD

PMID:16606699[7]

Experiment(s):EBI-1143175

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MHPMLNIAVR AARKAGNLIA KNYETPDAVE ASQKGSNDFV TNVDKAAEAV IIDTIRKSYP
QHTIITEESG ELEGTDQDVQ WVIDPLDGTT NFIKRLPHFA VSIAVRIKGR TEVAVVYDPM
RNELFTATRG QGAQLNGYRL RGSTARDLDG TILATGFPFK AKQYATTYIN IVGKLFNECA
DFRRTGSAAL DLAYVAAGRV DGFFEIGLRP WDFAAGELLV REAGGIVSDF TGGHNYMLTG
NIVAGNPRVV KAMLANMRDE LSDALKR
Length

267

Mol. Wt

29.172 kDa

pI

6.9 (calculated)

Extinction coefficient

22,920 - 23,045 (calc based on 8 Y, 2 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..263

PF00459 Inositol monophosphatase family

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=suhB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130458

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947285

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008337

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ADG4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10983

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10983

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947285

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000972

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0976

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 5.5 Chen, L & Roberts, MF (2000) Overexpression, purification, and analysis of complementation behavior of E. coli SuhB protein: comparison with bacterial and archaeal inositol monophosphatases. Biochemistry 39 4145-53 PubMed
  6. Wang, Y et al. (2007) The structure of the R184A mutant of the inositol monophosphatase encoded by suhB and implications for its functional interactions in Escherichia coli. J. Biol. Chem. 282 26989-96 PubMed
  7. 7.0 7.1 7.2 7.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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